Comparative studies on seed germination of two rice genotypes with different tolerances to low temperature

doi:10.1016/j.envexpbot.2020.104216

	Comparative studies on seed germination of two rice genotypes with different tolerances to low temperature
	Li, Qian ; Yang, An 1
	2020
发表期刊	ENVIRONMENTAL AND EXPERIMENTAL BOTANY
ISSN	0098-8472
卷号	179
摘要	Seed germination is a key phase of the plant life cycle, and the plant is highly sensitive to low temperature during this phase. However, the physiological and molecular mechanisms underlying the adaptation of rice seeds to low temperatures remain elusive. The present study compared seed germination between Dongdao-4 and Jigeng-88 and investigated the mechanisms of their response to low temperature conditions. Seed germination percentage of Dongdao-4 was significantly higher than that of Jigeng-88 when exposed to low temperature. Furthermore, Dongdao-4 seeds exhibited higher concentrations of bioactive gibberellins (GA(1)and GA(4)) under low temperature conditions compared to Jigeng-88 seeds, which was attributed to a higher induction of the expression of GA biosynthetic genes and a lower induction of the expression of GA inactivation genes at the transcriptional level in Dongdao-4 compared to those in Jigeng-88 seeds. We further demonstrated that GA treatment promoted seed germination under low temperature conditions, and GA biosynthetic inhibitors inhibited germination. During seed germination, soluble sugars are converted from starch by a-amylase to provide energy and fuel embryo development. The reduction in the germination percentage is accompanied by a reduction in a-amylase activity and soluble sugar concentrations under low temperature conditions. We observed higher a-amylase activity and soluble sugar levels in Dongdao-4 than in Jigeng-88 seeds under low temperature conditions. More soluble sugars were transported to embryos in Dongdao-4 seeds than in Jigeng-88 seeds under low temperature conditions, which was attributed to a higher transcriptional level of sugar transporters in Dongdao-4 than that in Jigeng-88. Abscisic acid (ABA) acts antagonistically compared to GA during seed germination. Lower endogenous concentrations of ABA in Dongdao-4 compared to those in Jigeng-88 seeds were found, because of the much higher transcriptional levels of ABA catabolism genes in Dongdao-4 than Jigeng-88 seeds when they were treated at an identical low temperature. These results suggest that the higher accumulation of endogenous bioactive GAs and soluble sugars and lower ABA concentrations in Dongdao-4 than Jigeng-88 were responsible for its increased seed germination under low temperature conditions.
关键词	Rice germination Low temperature Gibberellins Abscisic acid alpha-Amylase Soluble sugar
学科领域	Plant Sciences ; Environmental Sciences
DOI	10.1016/j.envexpbot.2020.104216
收录类别	SCI
语种	英语
WOS关键词	QUANTITATIVE TRAIT LOCUS ; TRANSPORTER GENE FAMILY ; ALPHA-AMYLASE ACTIVITY ; ABSCISIC-ACID ; SUCROSE TRANSPORTER ; SALT STRESS ; EXPRESSION ANALYSIS ; GREATER TOLERANCE ; NATURAL VARIATION ; SALINITY
WOS研究方向	Plant Sciences ; Environmental Sciences & Ecology
WOS记录号	WOS:000571434200004
出版者	PERGAMON-ELSEVIER SCIENCE LTD
文献子类	Article
出版地	OXFORD
EISSN	1873-7307
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31801280, 31301832] ; Fundamental Research Funds for the Central UniversitiesFundamental Research Funds for the Central Universities [2452018114] ; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2019M653770]
作者邮箱	anyang@ibcas.ac.cn
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21581
专题	植被与环境变化国家重点实验室
作者单位	1.Northwest A&F Univ, Coll Hort, Yangling, Shaanxi, Peoples R China 2.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Li, Qian,Yang, An. Comparative studies on seed germination of two rice genotypes with different tolerances to low temperature[J]. ENVIRONMENTAL AND EXPERIMENTAL BOTANY,2020,179.
APA	Li, Qian,&Yang, An.(2020).Comparative studies on seed germination of two rice genotypes with different tolerances to low temperature.ENVIRONMENTAL AND EXPERIMENTAL BOTANY,179.
MLA	Li, Qian,et al."Comparative studies on seed germination of two rice genotypes with different tolerances to low temperature".ENVIRONMENTAL AND EXPERIMENTAL BOTANY 179(2020).

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
1-s2.0-S009884722030（2407KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文