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Comparative studies on seed germination of two rice genotypes with different tolerances to low temperature
Li, Qian; Yang, An1
2020
Source PublicationENVIRONMENTAL AND EXPERIMENTAL BOTANY
ISSN0098-8472
Volume179
AbstractSeed germination is a key phase of the plant life cycle, and the plant is highly sensitive to low temperature during this phase. However, the physiological and molecular mechanisms underlying the adaptation of rice seeds to low temperatures remain elusive. The present study compared seed germination between Dongdao-4 and Jigeng-88 and investigated the mechanisms of their response to low temperature conditions. Seed germination percentage of Dongdao-4 was significantly higher than that of Jigeng-88 when exposed to low temperature. Furthermore, Dongdao-4 seeds exhibited higher concentrations of bioactive gibberellins (GA(1)and GA(4)) under low temperature conditions compared to Jigeng-88 seeds, which was attributed to a higher induction of the expression of GA biosynthetic genes and a lower induction of the expression of GA inactivation genes at the transcriptional level in Dongdao-4 compared to those in Jigeng-88 seeds. We further demonstrated that GA treatment promoted seed germination under low temperature conditions, and GA biosynthetic inhibitors inhibited germination. During seed germination, soluble sugars are converted from starch by a-amylase to provide energy and fuel embryo development. The reduction in the germination percentage is accompanied by a reduction in a-amylase activity and soluble sugar concentrations under low temperature conditions. We observed higher a-amylase activity and soluble sugar levels in Dongdao-4 than in Jigeng-88 seeds under low temperature conditions. More soluble sugars were transported to embryos in Dongdao-4 seeds than in Jigeng-88 seeds under low temperature conditions, which was attributed to a higher transcriptional level of sugar transporters in Dongdao-4 than that in Jigeng-88. Abscisic acid (ABA) acts antagonistically compared to GA during seed germination. Lower endogenous concentrations of ABA in Dongdao-4 compared to those in Jigeng-88 seeds were found, because of the much higher transcriptional levels of ABA catabolism genes in Dongdao-4 than Jigeng-88 seeds when they were treated at an identical low temperature. These results suggest that the higher accumulation of endogenous bioactive GAs and soluble sugars and lower ABA concentrations in Dongdao-4 than Jigeng-88 were responsible for its increased seed germination under low temperature conditions.
KeywordRice germination Low temperature Gibberellins Abscisic acid alpha-Amylase Soluble sugar
Subject AreaPlant Sciences ; Environmental Sciences
DOI10.1016/j.envexpbot.2020.104216
Indexed BySCI
Language英语
WOS KeywordQUANTITATIVE TRAIT LOCUS ; TRANSPORTER GENE FAMILY ; ALPHA-AMYLASE ACTIVITY ; ABSCISIC-ACID ; SUCROSE TRANSPORTER ; SALT STRESS ; EXPRESSION ANALYSIS ; GREATER TOLERANCE ; NATURAL VARIATION ; SALINITY
WOS Research AreaPlant Sciences ; Environmental Sciences & Ecology
WOS IDWOS:000571434200004
PublisherPERGAMON-ELSEVIER SCIENCE LTD
SubtypeArticle
Publication PlaceOXFORD
EISSN1873-7307
Funding OrganizationNational Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31801280, 31301832] ; Fundamental Research Funds for the Central UniversitiesFundamental Research Funds for the Central Universities [2452018114] ; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2019M653770]
Corresponding Author Emailanyang@ibcas.ac.cn
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Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ibcas.ac.cn/handle/2S10CLM1/21581
Collection植被与环境变化国家重点实验室
Affiliation1.Northwest A&F Univ, Coll Hort, Yangling, Shaanxi, Peoples R China
2.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China
Recommended Citation
GB/T 7714
Li, Qian,Yang, An. Comparative studies on seed germination of two rice genotypes with different tolerances to low temperature[J]. ENVIRONMENTAL AND EXPERIMENTAL BOTANY,2020,179.
APA Li, Qian,&Yang, An.(2020).Comparative studies on seed germination of two rice genotypes with different tolerances to low temperature.ENVIRONMENTAL AND EXPERIMENTAL BOTANY,179.
MLA Li, Qian,et al."Comparative studies on seed germination of two rice genotypes with different tolerances to low temperature".ENVIRONMENTAL AND EXPERIMENTAL BOTANY 179(2020).
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