An innovative artificial photosystem II constructed from PSII core of Thermosynechococcus vulcanus and LHCII of Pisum sativum - A new approach for studying the function of photosynthetic antenna

doi:10.1016/j.plaphy.2020.05.030

	An innovative artificial photosystem II constructed from PSII core of Thermosynechococcus vulcanus and LHCII of Pisum sativum - A new approach for studying the function of photosynthetic antenna
	Li, Ling 1; Li, Fei 1; Wang, Yue 1; Yang, Chunhong 1,2; Lu, Congming 3; Liu, Cheng 1; Sun, Ruixue 2
	2020
发表期刊	PLANT PHYSIOLOGY AND BIOCHEMISTRY
ISSN	0981-9428
卷号	154 页码:160-170
摘要	In photosynthesis, the antenna system captures solar energy and transfers the excitations to photosystem II (PSII) core complex where charge separation, water splitting and oxygen evolution occur. In the evolution of photosynthesis from aquatic to terrestrial environments, the structure of PSII core complex was highly conserved while a variety of antenna forms became differentiated. In order to study the principles for energy transport from antenna to the PSII reaction center, we have explored whether the major light harvesting complex of PSII (LHCII) of higher plants can transfer energy to the cyanobacteria PSII core complexes (CC). For this purpose, LHCII from pea and CC from Thermosynechococcus vulcanus were isolated and co-reconstituted into liposome at LHCII:CC molar ratios of 2:1, 4:1 and 6:1, respectively. Chemical-cross linking followed by LC-MS/MS analysis confirmed the biochemical interaction between LHCII and CC in the liposome membrane. The analyses of 77 K fluorescence emission spectra and antenna cross section of PSII indicated that LHCII can transfer energy directly to the cyanobacterial CC. The study has laid the basis for further research on the mechanism of energy transfer from LHCII to PSII CC. This result may also open a new possibility for design and development of new artificial PSII in the application of solar energy conversion.
关键词	Photosystem II LHCII Proteoliposome Energy transfer
学科领域	Plant Sciences
DOI	10.1016/j.plaphy.2020.05.030
收录类别	SCI
语种	英语
WOS关键词	LIGHT-HARVESTING COMPLEX ; PROTEIN COMPLEXES ; CRYSTAL-STRUCTURE ; ENERGY-TRANSFER ; EVOLUTION ; IDENTIFICATION ; STABILITY ; ISOFORMS ; MUTANTS ; PLANTS
WOS研究方向	Plant Sciences
WOS记录号	WOS:000566818900016
出版者	ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
文献子类	Article
出版地	ISSY-LES-MOULINEAUX
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31570236, 31370275] ; National Key R&D Program of China [2017YFA0503701]
作者邮箱	yangch@ibcas.ac.cn ; liucheng@ibcas.ac.cn ; sunrxchina@126.com
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21594
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing Bot Garden, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Yuquan Rd 19A, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Qingdao Inst, Shanghai Inst Technol Phys, Binhai Rd 50, Qingdao 264000, Peoples R China 4.Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Li, Ling,Li, Fei,Wang, Yue,et al. An innovative artificial photosystem II constructed from PSII core of Thermosynechococcus vulcanus and LHCII of Pisum sativum - A new approach for studying the function of photosynthetic antenna[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY,2020,154:160-170.
APA	Li, Ling.,Li, Fei.,Wang, Yue.,Yang, Chunhong.,Lu, Congming.,...&Sun, Ruixue.(2020).An innovative artificial photosystem II constructed from PSII core of Thermosynechococcus vulcanus and LHCII of Pisum sativum - A new approach for studying the function of photosynthetic antenna.PLANT PHYSIOLOGY AND BIOCHEMISTRY,154,160-170.
MLA	Li, Ling,et al."An innovative artificial photosystem II constructed from PSII core of Thermosynechococcus vulcanus and LHCII of Pisum sativum - A new approach for studying the function of photosynthetic antenna".PLANT PHYSIOLOGY AND BIOCHEMISTRY 154(2020):160-170.

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