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| An innovative artificial photosystem II constructed from PSII core of Thermosynechococcus vulcanus and LHCII of Pisum sativum - A new approach for studying the function of photosynthetic antenna | |
| Li, Ling1; Li, Fei1; Wang, Yue1; Yang, Chunhong1,2; Lu, Congming3; Liu, Cheng1; Sun, Ruixue2 | |
| 2020 | |
| Source Publication | PLANT PHYSIOLOGY AND BIOCHEMISTRY
 |
| ISSN | 0981-9428 |
| Volume | 154Pages:160-170 |
| Abstract | In photosynthesis, the antenna system captures solar energy and transfers the excitations to photosystem II (PSII) core complex where charge separation, water splitting and oxygen evolution occur. In the evolution of photosynthesis from aquatic to terrestrial environments, the structure of PSII core complex was highly conserved while a variety of antenna forms became differentiated. In order to study the principles for energy transport from antenna to the PSII reaction center, we have explored whether the major light harvesting complex of PSII (LHCII) of higher plants can transfer energy to the cyanobacteria PSII core complexes (CC). For this purpose, LHCII from pea and CC from Thermosynechococcus vulcanus were isolated and co-reconstituted into liposome at LHCII:CC molar ratios of 2:1, 4:1 and 6:1, respectively. Chemical-cross linking followed by LC-MS/MS analysis confirmed the biochemical interaction between LHCII and CC in the liposome membrane. The analyses of 77 K fluorescence emission spectra and antenna cross section of PSII indicated that LHCII can transfer energy directly to the cyanobacterial CC. The study has laid the basis for further research on the mechanism of energy transfer from LHCII to PSII CC. This result may also open a new possibility for design and development of new artificial PSII in the application of solar energy conversion. |
| Keyword | Photosystem II LHCII Proteoliposome Energy transfer |
| Subject Area | Plant Sciences |
| DOI | 10.1016/j.plaphy.2020.05.030 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | LIGHT-HARVESTING COMPLEX ; PROTEIN COMPLEXES ; CRYSTAL-STRUCTURE ; ENERGY-TRANSFER ; EVOLUTION ; IDENTIFICATION ; STABILITY ; ISOFORMS ; MUTANTS ; PLANTS |
| WOS Research Area | Plant Sciences |
| WOS ID | WOS:000566818900016 |
| Publisher | ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER |
| Subtype | Article |
| Publication Place | ISSY-LES-MOULINEAUX |
| Funding Organization | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31570236, 31370275] ; National Key R&D Program of China [2017YFA0503701] |
| Corresponding Author Email | yangch@ibcas.ac.cn ; liucheng@ibcas.ac.cn ; sunrxchina@126.com |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/21594 |
| Collection | 中科院北方资源植物重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing Bot Garden, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Yuquan Rd 19A, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Qingdao Inst, Shanghai Inst Technol Phys, Binhai Rd 50, Qingdao 264000, Peoples R China 4.Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China |
| Recommended Citation GB/T 7714 | Li, Ling,Li, Fei,Wang, Yue,et al. An innovative artificial photosystem II constructed from PSII core of Thermosynechococcus vulcanus and LHCII of Pisum sativum - A new approach for studying the function of photosynthetic antenna[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY,2020,154:160-170. |
| APA | Li, Ling.,Li, Fei.,Wang, Yue.,Yang, Chunhong.,Lu, Congming.,...&Sun, Ruixue.(2020).An innovative artificial photosystem II constructed from PSII core of Thermosynechococcus vulcanus and LHCII of Pisum sativum - A new approach for studying the function of photosynthetic antenna.PLANT PHYSIOLOGY AND BIOCHEMISTRY,154,160-170. |
| MLA | Li, Ling,et al."An innovative artificial photosystem II constructed from PSII core of Thermosynechococcus vulcanus and LHCII of Pisum sativum - A new approach for studying the function of photosynthetic antenna".PLANT PHYSIOLOGY AND BIOCHEMISTRY 154(2020):160-170. |
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| File Name/Size | DocType | Version | Access | License | ||
| 1-s2.0-S098194282030（2940KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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