Cooperation between Broussonetia papyrifera and Its Symbiotic Fungal Community To Improve Local Adaptation of the Host

doi:10.1128/AEM.00464-20

	Cooperation between Broussonetia papyrifera and Its Symbiotic Fungal Community To Improve Local Adaptation of the Host
	Chen, Peilin 1; Hu, Yanmin 1; Tang, Feng 1; Zhao, Meiling 1; Peng, Xianjun ; Shen, Shihua
	2020
发表期刊	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN	0099-2240
卷号	86 期号:18
摘要	The genetic basis of plant local adaptation has been extensively studied, yet the interplay between local adaptation, plant genetic divergence, and the microbial community remains unclear. Our study used the restriction-site associated DNA sequencing (RAD-seq) approach to explore genetic divergence in Broussonetia papyrifera and used internal transcribed spacers (ITS) to characterize fungal community. RAD-seq results show that B. papyrifera individuals could be divided into three genotypes; this genotyping result was consistent with the classification of climate type at the sample site. Most of the 101 highly differentiated genes were related to stress resistance and the microbiome. Moreover, p-diversity results indicated that genetic divergence had a significant effect on fungal community across all compartments (P < 0.01). At genus and operational taxonomic unit (OTU) level, Mortierella, Hannaella oryzae, OTU81578 (Mortierella), and OTU1665209 (H. oryzae) were found to be the major OTUs that contribute to differences in fungal community. The properties of cooccurrence networks vary greatly among three genotypes. The results of redundancy analysis (RDA) indicated that B. papyrifera-associated fungal community was significantly related to its local adaptability. Our findings suggest that genetic divergence of 8. papyrifera is closely related to local adaptation, with significant effects on the associated fungal community, which in turn would enhance host local adaptability. This improves present understanding about the coevolution of microbial communities and the host plant. IMPORTANCE The coevolution of plants with the associated fungal community and its effect on plant adaptability are not clear, especially for native trees. This study focuses on the genetic basis of local adaptation in plants and the effect of genetic divergence of Broussonetia papyrifera on the associated fungal community. We identified genes related to the microbiome that are important for local adaptation of the host. Our results show that genetic divergence in B. papyrifera significantly affects the fungal community, which has a close connection with local adaptation. This helps us to understand the relationship between local adaptation, genetic divergence, and associated fungal communities. This study highlights the effect of plant genetic divergence on associated fungal community for native trees and establishes a close connection between this effect and local adaptability in the host. In addition, these observations lay a foundation for the research of coevolution of plants and their symbiotic microbiome through genome-wide association study (GWAS).
关键词	B. papyrifera coevolution co-occurrence network fungal community genetic divergence local adaptations
学科领域	Biotechnology & Applied Microbiology ; Microbiology
DOI	10.1128/AEM.00464-20
收录类别	SCI
语种	英语
WOS关键词	RHIZOSPHERE MICROBIOME ; DIVERSITY ; BACTERIAL ; SOIL ; PATTERNS ; LANDSCAPE ; GENOTYPE ; GENETICS ; WILD ; LEAF
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
WOS记录号	WOS:000566699700003
出版者	AMER SOC MICROBIOLOGY
文献子类	Article
出版地	WASHINGTON
EISSN	1098-5336
资助机构	ChuangGou Science & Technology Co., Ltd., Beijing, China
作者邮箱	pengxianjun@ibcas.ac.cn ; shshen@ibcas.ac.cn
作品OA属性	Green Published
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21595
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Chen, Peilin,Hu, Yanmin,Tang, Feng,et al. Cooperation between Broussonetia papyrifera and Its Symbiotic Fungal Community To Improve Local Adaptation of the Host[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2020,86(18).
APA	Chen, Peilin,Hu, Yanmin,Tang, Feng,Zhao, Meiling,Peng, Xianjun,&Shen, Shihua.(2020).Cooperation between Broussonetia papyrifera and Its Symbiotic Fungal Community To Improve Local Adaptation of the Host.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,86(18).
MLA	Chen, Peilin,et al."Cooperation between Broussonetia papyrifera and Its Symbiotic Fungal Community To Improve Local Adaptation of the Host".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 86.18(2020).

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