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Characterization of a 2,3-oxidosqualene cyclase in the toosendanin biosynthetic pathway ofMelia toosendan
Lian, Xufan; Zhang, Xiuli1,2; Wang, Fei3; Wang, Xiaoning4; Xue, Zheyong1,2; Qi, Xiaoquan
AbstractToosendanin, bearing a furan ring, is a limonoid belonging to the group of tetranortriterpenoids. Toosendanin is a phytochemical found in the medicinal plantMelia toosendanSieb.etZucc. of the Meliaceae family. Toosendanin and its derivatives demonstrate high insecticidal activity and are important pesticides derived from plants. Despite intensive investigation of limonoids over several decades, the biosynthetic pathway of these triterpenoids is less understood. To identify the key enzymes involved in the toosendanin biosynthetic pathway, we analyzed the contents of toosendanin in various plant tissues and parts and found that the highest level of toosendanin was found in the developing fruit and gradually decreased as the fruit matured. More than 346 116 transcripts were assembled based on 394 million paired-end Illumina reads and 6 million PacBio reads from the pooled RNA samples of fruits, leaves and young barks. A total of 186 263 genes were predicted. Six 2,3-oxidosqualene cyclase (OSC) genes were identified by analyzing the association between gene expression and metabolite profiles. Functional analyses using theNicotiana benthamianatransient expression assay showed thatMtOSC1catalyzed 2,3-oxidosqualene to produce a tetracyclic triterpene skeleton, tirucalla-7,24-dien-3 beta-ol, which is predicted as the precursor for toosendanin biosynthesis. We identified another OSC, MtOSC6, which is a lupeol synthase. Using synthetic biology methods, these identified enzymes could be used to model a biosynthetic pathway to produce large quantities of toosendanin.
Subject AreaPlant Sciences
Indexed BySCI
WOS Research AreaPlant Sciences
WOS IDWOS:000565153300001
Publication PlaceHOBOKEN
Funding OrganizationNational Key R&D Program of China [2017YFD0201405] ; Heilongjiang Touyan Innovation Team Program (Tree Genetics and Breeding Innovation Team) ; National Natural Science Foundation of China (NSFC)National Natural Science Foundation of China (NSFC) [31770332, 31970314]
Corresponding Author Emailzyxue@nefu.edu.cn ; xqi@ibcas.ac.cn
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Affiliation1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
2.Northeast Forestry Univ, Minist Educ, Key Lab Saline Alkali Vegetat Ecol Restorat, Harbin 150040, Peoples R China
3.Northeast Forestry Univ, Heilongjiang Key Lab Plant Bioact Subst Biosynth, Harbin 150040, Peoples R China
4.Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China
5.Shandong Univ, Sch Pharmaceut Sci, Minist Educ, Dept Nat Prod Chem,Key Lab Chem Biol, Jinan 250012, Peoples R China
Recommended Citation
GB/T 7714
Lian, Xufan,Zhang, Xiuli,Wang, Fei,et al. Characterization of a 2,3-oxidosqualene cyclase in the toosendanin biosynthetic pathway ofMelia toosendan[J]. PHYSIOLOGIA PLANTARUM,2020,170(4):528-536.
APA Lian, Xufan,Zhang, Xiuli,Wang, Fei,Wang, Xiaoning,Xue, Zheyong,&Qi, Xiaoquan.(2020).Characterization of a 2,3-oxidosqualene cyclase in the toosendanin biosynthetic pathway ofMelia toosendan.PHYSIOLOGIA PLANTARUM,170(4),528-536.
MLA Lian, Xufan,et al."Characterization of a 2,3-oxidosqualene cyclase in the toosendanin biosynthetic pathway ofMelia toosendan".PHYSIOLOGIA PLANTARUM 170.4(2020):528-536.
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