IB-CAS  > 中科院植物分子生理学重点实验室
Identification and genetic analysis of EMS-mutagenized wheat mutants conferring lesion-mimic premature aging
Kong, Weiwei; Wang, Liming; Cao, Pei1; Li, Xingfeng2; Ji, Jingjing; Dong, Puhui; Yan, Xuefang; Wang, Chunping; Wang, Honggang2; Sun, Jiaqiang3
2020
Source PublicationBMC GENETICS
ISSN1471-2156
Volume21Issue:1
AbstractBackground Lesion-mimic and premature aging (lmpa) mutantlmpa1was identified from the ethyl methane sulfonate (EMS) mutant library in the bread wheat variety Keda 527 (KD527) background. To reveal the genetic basis oflmpa1mutant, phenotypic observations and analyses of chlorophyll content and photosynthesis were carried out inlmpa1, KD527 and their F(1)and F(2)derivatives. Further, bulked segregation analysis (BSA) in combination with a 660 K SNP array were conducted on the F(2)segregation population oflmpa1/Chinese spring (CS) to locate thelmpa1gene. Results Most agronomic traits oflmpa1were similar to those of KD527 before lesion-like spots appeared. Genetic analysis indicated that the F(1)plants from the crossing oflmpa1and KD527 exhibited thelmpaphenotype and the F(2)progenies showed a segregation of normal (wild type, WT) andlmpa, with the ratios oflmpa: WT = 124:36(chi(2) = 1.008 < =3.841), indicating thatlmpais a dominant mutation. The combination of BSA and the SNP array analysis of CS,lmpa1andlmpa1/CS F2WT pool (50 plants) andlmpapool (50 plants) showed that polymorphic SNPs were enriched on chromosome 5A, within a region of 30-40 Mb, indicating that the wheat premature aging geneLmpa1was probably located on the short arm of chromosome 5A. Conclusions EMS-mutagenized mutantlmpa1 deriving from elite wheat line KD527 conferredlmpa.Lmpaphenotype oflmpa1 mutant is controlled by a single dominant allele designated asLmpa1, which affected wheat growth and development and reduced the thousand grain weight (tgw) of single plant in wheat. The geneLmpa1was tentatively located within the region of 30-40 Mb near to the short arm of chromosome 5A.
KeywordWheat lmpa1 Mutant Chromosomal location
Subject AreaGenetics & Heredity
DOI10.1186/s12863-020-00891-x
Indexed BySCI
Language英语
WOS KeywordCELL-DEATH ; LEAF SENESCENCE ; POWDERY MILDEW ; BSR-SEQ ; RESISTANCE
WOS Research AreaGenetics & Heredity
WOS IDWOS:000563546300001
PublisherBMC
SubtypeArticle
Publication PlaceLONDON
Funding OrganizationNational Natural Science Foundation of China-Henan Joint fund [U1304318, U1904108] ; Scientific and technological projects of Henan Province of China [202102110022]
Corresponding Author Emaillmwang1@163.com ; sunjiaqiang@caas.cn
OAgold, Green Submitted, Green Published
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Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ibcas.ac.cn/handle/2S10CLM1/21610
Collection中科院植物分子生理学重点实验室
Affiliation1.Henan Univ Sci & Technol, Agron Coll, Luoyang 471023, Henan, Peoples R China
2.Chinese Acad Sci, Inst Bot, Beijing 10093, Peoples R China
3.Shandong Agr Univ, Agron Coll, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China
4.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 10081, Peoples R China
Recommended Citation
GB/T 7714
Kong, Weiwei,Wang, Liming,Cao, Pei,et al. Identification and genetic analysis of EMS-mutagenized wheat mutants conferring lesion-mimic premature aging[J]. BMC GENETICS,2020,21(1).
APA Kong, Weiwei.,Wang, Liming.,Cao, Pei.,Li, Xingfeng.,Ji, Jingjing.,...&Sun, Jiaqiang.(2020).Identification and genetic analysis of EMS-mutagenized wheat mutants conferring lesion-mimic premature aging.BMC GENETICS,21(1).
MLA Kong, Weiwei,et al."Identification and genetic analysis of EMS-mutagenized wheat mutants conferring lesion-mimic premature aging".BMC GENETICS 21.1(2020).
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