Grazing does not increase soil antibiotic resistome in two types of grasslands in Inner Mongolia, China

doi:10.1016/j.apsoil.2020.103644

	Grazing does not increase soil antibiotic resistome in two types of grasslands in Inner Mongolia, China
	Du, Shuai ; Shen, Ju-Pei ; Sun, Yi-Fei ; Bai, Yong-Fei; Pan, Hong 4; Li, Yong 5; Wang, Zhong-Wu ; Han, Guo-Dong ; Zhang, Li-Mei ; He, Ji-Zheng
	2020
发表期刊	APPLIED SOIL ECOLOGY
ISSN	0929-1393
卷号	155
摘要	Long-term livestock grazing often comes with inputs of manure and urine of animals to grassland soils and can influence the microbial communities. However, the effects of grazing on the diversity and abundance of soil antibiotic resistance genes (ARGs) are still obscure. Here we profiled soil resistome using high-throughput quantitative PCR analysis from desert and typical grasslands (Siziwang and Xilingol steppe, respectively) in Inner Mongolia, each with four grazing intensities (no grazing, light grazing, moderate grazing, and heavy grazing). Totally, 54 and 39 ARGs were detected in the Siziwang steppe and the Xilingol steppe, respectively. The relative abundance of ARGs significantly decreased in the Siziwang steppe or remained unchanged in the Xilingol steppe along the gradient of increasing grazing intensity. Mobile genetic elements (MGEs) were significantly and positively correlated with ARGs in the Siziwang steppe but not in the Xilingol steppe. Structural equation model revealed that grazing indirectly affected ARGs through its impact on MGEs, bacterial diversity, and bacterial community composition. These findings suggest that livestock grazing can impact soil resistome via regulating microbiome in grassland soils. However, grazing intensity has not resulted in a significant impact on the soil resistomes in these desert and typical grasslands.
关键词	Grassland soil Grazing intensity Antibiotic resistance gene Mobile genetic elements Bacterial community
学科领域	Soil Science
DOI	10.1016/j.apsoil.2020.103644
收录类别	SCI
语种	英语
WOS关键词	RESISTANCE GENES ; BACTERIAL COMMUNITIES ; MICROBIAL COMMUNITY ; NITROGEN ADDITION ; CATTLE ; SUCCESSION ; INTENSITY ; RESPONSES ; IMPACTS ; MANURES
WOS研究方向	Agriculture
WOS记录号	WOS:000560743700019
出版者	ELSEVIER
文献子类	Article
出版地	AMSTERDAM
EISSN	1873-0272
资助机构	Program of Intergovernmental Cooperation in Science and Technology [2017YFE0109800] ; Ministry of Science and Technology of the People's Republic of ChinaMinistry of Science and Technology, China [2013CB956300] ; Innovative Team of Grassland Resources of the Ministry of Education of China [IRT_17R59] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31660679]
作者邮箱	jpshen@rcees.ac.cn
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21622
专题	植被与环境变化国家重点实验室
作者单位	1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.China Agr Univ, Coll Land Sci & Technol, Beijing 100193, Peoples R China 4.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China 5.Shandong Agr Univ, Coll Resources & Environm Sci, Natl Engn Lab Efficient Utilizat Soil & Fertilize, Tai An 271018, Shandong, Peoples R China 6.Zhejiang Univ, Inst Soil & Water Resources & Environm Sci, Zhejiang Prov Key Lab Agr Resources & Environm, Hangzhou 310058, Peoples R China 7.Inner Mongolia Agr Univ, Dept Grassland Sci, Coll Ecol & Environm Sci, Hohhot 010018, Peoples R China 8.Inner Mongolia Agr Univ, Inner Mongolia Key Lab Management & Utilizat, Key Lab Forage Cultivat Proc & High Efficient Uti, Minist Agr China,Coll Grassland Resources & Envir, Hohhot 010011, Peoples R China 9.Univ Melbourne, Sch Agr & Food, Fac Vet & Agr Sci, Parkville, Vic 3010, Australia
推荐引用方式 GB/T 7714	Du, Shuai,Shen, Ju-Pei,Sun, Yi-Fei,et al. Grazing does not increase soil antibiotic resistome in two types of grasslands in Inner Mongolia, China[J]. APPLIED SOIL ECOLOGY,2020,155.
APA	Du, Shuai.,Shen, Ju-Pei.,Sun, Yi-Fei.,Bai, Yong-Fei.,Pan, Hong.,...&He, Ji-Zheng.(2020).Grazing does not increase soil antibiotic resistome in two types of grasslands in Inner Mongolia, China.APPLIED SOIL ECOLOGY,155.
MLA	Du, Shuai,et al."Grazing does not increase soil antibiotic resistome in two types of grasslands in Inner Mongolia, China".APPLIED SOIL ECOLOGY 155(2020).

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