IB-CAS  > 中科院植物分子生理学重点实验室
The effect of phytoglobin overexpression on the plant proteome during nonhost response of barley (Hordeum vulgare) to wheat powdery mildew (Blumeria graminis f. sp. tritici)
Andrzejczak, O. A.; Sorensen, C. K.; Wang, W-Q; Kovalchuk, S.; Hagensen, C. E.; Jensen, O. N.; Carciofi, M.; Hovmoller, M. S.; Rogowska-Wrzesinska, A.; Moller, I. M.; Hebelstrup, K. H.
2020
发表期刊SCIENTIFIC REPORTS
ISSN2045-2322
卷号10期号:1
摘要Nonhost resistance, a resistance of plant species against all nonadapted pathogens, is considered the most durable and efficient immune system in plants. To increase our understanding of the response of barley plants to infection by powdery mildew, Blumeria graminis f. sp. tritici, we used quantitative proteomic analysis (LC-MS/MS). We compared the response of two genotypes of barley cultivar Golden Promise, wild type (WT) and plants with overexpression of phytoglobin (previously hemoglobin) class 1 (HO), which has previously been shown to significantly weaken nonhost resistance. A total of 8804 proteins were identified and quantified, out of which the abundance of 1044 proteins changed significantly in at least one of the four comparisons ('i' stands for 'inoculated')- HO/WT and HOi/WTi (giving genotype differences), and WTi/WT and HOi/HO (giving treatment differences). Among these differentially abundant proteins (DAP) were proteins related to structural organization, disease/defense, metabolism, transporters, signal transduction and protein synthesis. We demonstrate that quantitative changes in the proteome can explain physiological changes observed during the infection process such as progression of the mildew infection in HO plants that was correlated with changes in proteins taking part in papillae formation and preinvasion resistance. Overexpression of phytoglobins led to modification in signal transduction prominently by dramatically reducing the number of kinases induced, but also in the turnover of other signaling molecules such as phytohormones, polyamines and Ca2+. Thus, quantitative proteomics broaden our understanding of the role NO and phytoglobins play in barley during nonhost resistance against powdery mildew.
学科领域Multidisciplinary Sciences
DOI10.1038/s41598-020-65907-z
收录类别SCI
语种英语
WOS关键词NITRIC-OXIDE ; HYPERSENSITIVE RESPONSE ; ARABIDOPSIS-THALIANA ; DISEASE RESISTANCE ; HYDROGEN-PEROXIDE ; PLASMA-MEMBRANE ; HEMOGLOBIN ; EXPRESSION ; HOST ; DEFENSE
WOS研究方向Science & Technology - Other Topics
WOS记录号WOS:000560458900004
出版者NATURE RESEARCH
文献子类Article
出版地BERLIN
资助机构Danish Council for Independent Research/ Technology and Production SciencesDet Frie Forskningsrad (DFF) [4005-00073B] ; Novo Nordisk FoundationNovo Nordisk FoundationNovocure Limited [NNF18OC0052451] ; [7292] ; [5072-00007B]
作者邮箱kim.hebelstrup@mbg.au.dk
作品OA属性gold, Green Published
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/21625
专题中科院植物分子生理学重点实验室
作者单位1.[Andrzejczak, O. A.
2.Sorensen, C. K.
3.Carciofi, M.
4.Hovmoller, M. S.
5.Hebelstrup, K. H.] Aarhus Univ, Dept Agroecol, Forsogsvej 1, DK-4200 Slagelse, Denmark
6.[Wang, W-Q
7.Kovalchuk, S.
8.Hagensen, C. E.
9.Jensen, O. N.
10.Rogowska-Wrzesinska, A.] Univ Southern Denmark, Dept Biochem & Mol Biol, Campusvej 55, DK-5230 Odense M, Denmark
11.Rogowska-Wrzesinska, A.] Univ Southern Denmark, VILLUM Ctr Bioanalyt Sci, Campusvej 55, DK-5230 Odense M, Denmark
12.[Carciofi, M.
13.Moller, I. M.
14.Hebelstrup, K. H.] Aarhus Univ, Dept Mol Biol & Genet, Forsogsvej 1, DK-4200 Slagelse, Denmark
15.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
16.[Kovalchuk, S.] RAS, Lab Bioinformat Methods Combinato, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow, Russia
推荐引用方式
GB/T 7714
Andrzejczak, O. A.,Sorensen, C. K.,Wang, W-Q,et al. The effect of phytoglobin overexpression on the plant proteome during nonhost response of barley (Hordeum vulgare) to wheat powdery mildew (Blumeria graminis f. sp. tritici)[J]. SCIENTIFIC REPORTS,2020,10(1).
APA Andrzejczak, O. A..,Sorensen, C. K..,Wang, W-Q.,Kovalchuk, S..,Hagensen, C. E..,...&Hebelstrup, K. H..(2020).The effect of phytoglobin overexpression on the plant proteome during nonhost response of barley (Hordeum vulgare) to wheat powdery mildew (Blumeria graminis f. sp. tritici).SCIENTIFIC REPORTS,10(1).
MLA Andrzejczak, O. A.,et al."The effect of phytoglobin overexpression on the plant proteome during nonhost response of barley (Hordeum vulgare) to wheat powdery mildew (Blumeria graminis f. sp. tritici)".SCIENTIFIC REPORTS 10.1(2020).
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Andrzejczak-2020-The(9246KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Andrzejczak, O. A.]的文章
[Sorensen, C. K.]的文章
[Wang, W-Q]的文章
百度学术
百度学术中相似的文章
[Andrzejczak, O. A.]的文章
[Sorensen, C. K.]的文章
[Wang, W-Q]的文章
必应学术
必应学术中相似的文章
[Andrzejczak, O. A.]的文章
[Sorensen, C. K.]的文章
[Wang, W-Q]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Andrzejczak-2020-The effect of phytoglobin ove.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。