Knowledge Management System Of Institute Of Botany,CAS
Regulation of photosystem I-light-harvesting complex I from a red algaCyanidioschyzon merolaein response to light intensities | |
Chang, Lijing1; Tian, Lirong2; Ma, Fei; Mao, Zhiyuan1; Liu, Xiaochi1; Han, Guangye; Wang, Wenda; Yang, Yanyan; Kuang, Tingyun; Pan, Jie4; Shen, Jian-Ren | |
2020 | |
发表期刊 | PHOTOSYNTHESIS RESEARCH |
ISSN | 0166-8595 |
卷号 | 146期号:1-3页码:287-297 |
摘要 | Photosynthetic organisms use different means to regulate their photosynthetic activity in respond to different light conditions under which they grow. In this study, we analyzed changes in the photosystem I (PSI) light-harvesting complex I (LHCI) supercomplex from a red algaCyanidioschyzon merolae, upon growing under three different light intensities, low light (LL), medium light (ML), and high light (HL). The results showed that the red algal PSI-LHCI is separated into two bands on blue-native PAGE, which are designated PSI-LHCI-A and PSI-LHCI-B, respectively, from cells grown under LL and ML. The former has a higher molecular weight and binds more Lhcr subunits than the latter. They are considered to correspond to the two types of PSI-LHCI identified by cryo-electron microscopic analysis recently, namely, the former with five Lhcrs and the latter with three Lhcrs. The amount of PSI-LHCI-A is higher in the LL-grown cells than that in the ML-grown cells. In the HL-grown cells, PSI-LHCI-A completely disappeared and only PSI-LHCI-B was observed. Furthermore, PSI core complexes without Lhcr attached also appeared in the HL cells. Fluorescence decay kinetics measurement showed that Lhcrs are functionally connected with the PSI core in both PSI-LHCI-A and PSI-LHCI-B obtained from LL and ML cells; however, Lhcrs in the PSI-LHCI-B fraction from the HL cells are not coupled with the PSI core. These results indicate that the red algal PSI not only regulates its antenna size but also adjusts the functional connection of Lhcrs with the PSI core in response to different light intensities. |
关键词 | Light adaptation PSI-LHCI Light-harvesting antenna Fluorescence decay Red algae Cyanidioschyzon merolae |
学科领域 | Plant Sciences |
DOI | 10.1007/s11120-020-00778-z |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | LHCI SUPER-COMPLEX ; CYANIDIOSCHYZON-MEROLAE ; CYANIDIUM-CALDARIUM ; FUNCTIONAL-PROPERTIES ; EXTRINSIC PROTEINS ; OLIGOMERIC STATES ; 12-KDA PROTEIN ; SUB-COMPLEXES ; GREEN-ALGA ; ANTENNA |
WOS研究方向 | Plant Sciences |
WOS记录号 | WOS:000556637400001 |
出版者 | SPRINGER |
Special Issue | SI |
文献子类 | Article |
出版地 | DORDRECHT |
EISSN | 1573-5079 |
资助机构 | National Key R&D Program of China [2017YFA0503700] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31470339] ; Strategic Priority Research Program of CAS [XDB17000000] ; Key Research Program of Frontier Sciences, CAS [QYZDY-SSW-SMC003] ; The Innovative Academy for Seed Design, Chinese Academy of Sciences ; Natural Science Foundation of Hebei ProvinceNatural Science Foundation of Hebei Province [C2020205051] ; Doctoral fund of Hebei Normal University [L2020B20] ; Hebei Collaboration Innovation Center for Cell Signaling, China |
作者邮箱 | shen@cc.okayama-u.ac.jp |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/21642 |
专题 | 中科院光生物学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, Key Lab Photobiol, 20 Nanxincun, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Yuquan Rd, Beijing 100049, Peoples R China 3.Hebei Normal Univ, Coll Life Sci, Hebei Key Lab Mol & Cellular Biol, Shijiazhuang 050024, Hebei, Peoples R China 4.Okayama Univ, Res Inst Interdisciplinary Sci, Okayama 7008530, Japan 5.Michigan State Univ, Dept Chem, E Lansing, MI 48824 USA |
推荐引用方式 GB/T 7714 | Chang, Lijing,Tian, Lirong,Ma, Fei,et al. Regulation of photosystem I-light-harvesting complex I from a red algaCyanidioschyzon merolaein response to light intensities[J]. PHOTOSYNTHESIS RESEARCH,2020,146(1-3):287-297. |
APA | Chang, Lijing.,Tian, Lirong.,Ma, Fei.,Mao, Zhiyuan.,Liu, Xiaochi.,...&Shen, Jian-Ren.(2020).Regulation of photosystem I-light-harvesting complex I from a red algaCyanidioschyzon merolaein response to light intensities.PHOTOSYNTHESIS RESEARCH,146(1-3),287-297. |
MLA | Chang, Lijing,et al."Regulation of photosystem I-light-harvesting complex I from a red algaCyanidioschyzon merolaein response to light intensities".PHOTOSYNTHESIS RESEARCH 146.1-3(2020):287-297. |
条目包含的文件 | ||||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
Chang-2020-Regulatio(4355KB) | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论