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Regulation of photosystem I-light-harvesting complex I from a red algaCyanidioschyzon merolaein response to light intensities
Chang, Lijing1; Tian, Lirong2; Ma, Fei; Mao, Zhiyuan1; Liu, Xiaochi1; Han, Guangye; Wang, Wenda; Yang, Yanyan; Kuang, Tingyun; Pan, Jie4; Shen, Jian-Ren
2020
Source PublicationPHOTOSYNTHESIS RESEARCH
ISSN0166-8595
Volume146Issue:1-3Pages:287-297
AbstractPhotosynthetic organisms use different means to regulate their photosynthetic activity in respond to different light conditions under which they grow. In this study, we analyzed changes in the photosystem I (PSI) light-harvesting complex I (LHCI) supercomplex from a red algaCyanidioschyzon merolae, upon growing under three different light intensities, low light (LL), medium light (ML), and high light (HL). The results showed that the red algal PSI-LHCI is separated into two bands on blue-native PAGE, which are designated PSI-LHCI-A and PSI-LHCI-B, respectively, from cells grown under LL and ML. The former has a higher molecular weight and binds more Lhcr subunits than the latter. They are considered to correspond to the two types of PSI-LHCI identified by cryo-electron microscopic analysis recently, namely, the former with five Lhcrs and the latter with three Lhcrs. The amount of PSI-LHCI-A is higher in the LL-grown cells than that in the ML-grown cells. In the HL-grown cells, PSI-LHCI-A completely disappeared and only PSI-LHCI-B was observed. Furthermore, PSI core complexes without Lhcr attached also appeared in the HL cells. Fluorescence decay kinetics measurement showed that Lhcrs are functionally connected with the PSI core in both PSI-LHCI-A and PSI-LHCI-B obtained from LL and ML cells; however, Lhcrs in the PSI-LHCI-B fraction from the HL cells are not coupled with the PSI core. These results indicate that the red algal PSI not only regulates its antenna size but also adjusts the functional connection of Lhcrs with the PSI core in response to different light intensities.
KeywordLight adaptation PSI-LHCI Light-harvesting antenna Fluorescence decay Red algae Cyanidioschyzon merolae
Subject AreaPlant Sciences
DOI10.1007/s11120-020-00778-z
Indexed BySCI
Language英语
WOS KeywordLHCI SUPER-COMPLEX ; CYANIDIOSCHYZON-MEROLAE ; CYANIDIUM-CALDARIUM ; FUNCTIONAL-PROPERTIES ; EXTRINSIC PROTEINS ; OLIGOMERIC STATES ; 12-KDA PROTEIN ; SUB-COMPLEXES ; GREEN-ALGA ; ANTENNA
WOS Research AreaPlant Sciences
WOS IDWOS:000556637400001
PublisherSPRINGER
Special IssueSI
SubtypeArticle
Publication PlaceDORDRECHT
EISSN1573-5079
Funding OrganizationNational Key R&D Program of China [2017YFA0503700] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31470339] ; Strategic Priority Research Program of CAS [XDB17000000] ; Key Research Program of Frontier Sciences, CAS [QYZDY-SSW-SMC003] ; The Innovative Academy for Seed Design, Chinese Academy of Sciences ; Natural Science Foundation of Hebei ProvinceNatural Science Foundation of Hebei Province [C2020205051] ; Doctoral fund of Hebei Normal University [L2020B20] ; Hebei Collaboration Innovation Center for Cell Signaling, China
Corresponding Author Emailshen@cc.okayama-u.ac.jp
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ibcas.ac.cn/handle/2S10CLM1/21642
Collection中科院光生物学重点实验室
Affiliation1.Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, Key Lab Photobiol, 20 Nanxincun, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Yuquan Rd, Beijing 100049, Peoples R China
3.Hebei Normal Univ, Coll Life Sci, Hebei Key Lab Mol & Cellular Biol, Shijiazhuang 050024, Hebei, Peoples R China
4.Okayama Univ, Res Inst Interdisciplinary Sci, Okayama 7008530, Japan
5.Michigan State Univ, Dept Chem, E Lansing, MI 48824 USA
Recommended Citation
GB/T 7714
Chang, Lijing,Tian, Lirong,Ma, Fei,et al. Regulation of photosystem I-light-harvesting complex I from a red algaCyanidioschyzon merolaein response to light intensities[J]. PHOTOSYNTHESIS RESEARCH,2020,146(1-3):287-297.
APA Chang, Lijing.,Tian, Lirong.,Ma, Fei.,Mao, Zhiyuan.,Liu, Xiaochi.,...&Shen, Jian-Ren.(2020).Regulation of photosystem I-light-harvesting complex I from a red algaCyanidioschyzon merolaein response to light intensities.PHOTOSYNTHESIS RESEARCH,146(1-3),287-297.
MLA Chang, Lijing,et al."Regulation of photosystem I-light-harvesting complex I from a red algaCyanidioschyzon merolaein response to light intensities".PHOTOSYNTHESIS RESEARCH 146.1-3(2020):287-297.
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