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| Crystal structure of Arabidopsis thaliana cytidine deaminase | |
| Wang, Jia1; Guo, Qi1; Liu, Lin2; Wang, Xiao2 | |
| 2020 | |
| Source Publication | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| ISSN | 0006-291X |
| Volume | 529Issue:3Pages:659-665 |
| Abstract | Cytidine deaminase (CDA) catalyzes the (deoxy)cytidine deamination to (deoxy)uridine, which involves in the catabolic and salvage pathways of pyrimidine nucleotides in plants. CDA serves as a prototype of the cytidine deaminase superfamily that contains a number of RNA editing enzymes. Arabidopsis thaliana has only one functional CDA, AtCDA1. We solved the crystal structures of AtCDA1, which is a dimeric zinccontaining enzyme and each protomer consists of an N-terminal zinc-binding catalytic domain and a C-terminal non-catalytic domain. Both domains adopt a typical alpha/beta/alpha sandwich fold. In vitro biochemical assays showed that the ribose moiety of cytidine is required for ligand binding, and structural analyses revealed a conserved catalytic mechanism is adopted by AtCDA1. (C) 2020 Elsevier Inc. All rights reserved. |
| Keyword | Pyrimidine metabolism RNA editing Crystallography Catalytic mechanism |
| Subject Area | Biochemistry & Molecular Biology ; Biophysics |
| DOI | 10.1016/j.bbrc.2020.06.084 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | FAMILY PROTEINS ; CHLOROPHYLL-A ; PYRIMIDINE ; ENZYME ; MODEL |
| WOS Research Area | Biochemistry & Molecular Biology ; Biophysics |
| WOS ID | WOS:000555780500022 |
| Publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| Subtype | Article |
| Publication Place | SAN DIEGO |
| EISSN | 1090-2104 |
| Funding Organization | National Key R&D Program of China [2017YFA0503703] ; China Postdoctoral Science FundChina Postdoctoral Science Foundation [2018M632517] ; Provincial Natural Science Foundation of Anhui [1908085QC129] |
| Corresponding Author Email | 19130@ahu.edu.cn |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/21644 |
| Collection | 中科院光生物学重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Inst Bot, CAS Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Anhui Univ, Sch Life Sci, Duxing North Bldg,Rm B305,111 Jiulong Rd, Hefei 230601, Anhui, Peoples R China |
| Recommended Citation GB/T 7714 | Wang, Jia,Guo, Qi,Liu, Lin,et al. Crystal structure of Arabidopsis thaliana cytidine deaminase[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2020,529(3):659-665. |
| APA | Wang, Jia,Guo, Qi,Liu, Lin,&Wang, Xiao.(2020).Crystal structure of Arabidopsis thaliana cytidine deaminase.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,529(3),659-665. |
| MLA | Wang, Jia,et al."Crystal structure of Arabidopsis thaliana cytidine deaminase".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 529.3(2020):659-665. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| 1-s2.0-S0006291X2031（1881KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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