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Phase separation of Arabidopsis EMB1579 controls transcription, mRNA splicing, and development
Zhang, Yiling; Li, Zhankun; Chen, Naizhi1; Huang, Yao; Huang, Shanjin
2020
发表期刊PLOS BIOLOGY
ISSN1544-9173
卷号18期号:7
摘要Tight regulation of gene transcription and mRNA splicing is essential for plant growth and development. Here we demonstrate that a plant-specific protein, EMBRYO DEFECTIVE 1579 (EMB1579), controls multiple growth and developmental processes inArabidopsis. We demonstrate that EMB1579 forms liquid-like condensates both in vitro and in vivo, and the formation of normal-sized EMB1579 condensates is crucial for its cellular functions. We found that some chromosomal and RNA-related proteins interact with EMB1579 compartments, and loss of function ofEMB1579affects global gene transcription and mRNA splicing. Using floral transition as a physiological process, we demonstrate that EMB1579 is involved inFLOWERING LOCUS C(FLC)-mediated repression of flowering. Interestingly, we found that EMB1579 physically interacts with a homologue ofDrosophilanucleosome remodeling factor 55-kDa (p55) called MULTIPLE SUPPRESSOR OF IRA 4 (MSI4), which has been implicated in repressing the expression ofFLCby forming a complex with DNA Damage Binding Protein 1 (DDB1) and Cullin 4 (CUL4). This complex, named CUL4-DDB1(MSI4), physically associates with a CURLY LEAF (CLF)-containing Polycomb Repressive Complex 2 (CLF-PRC2). We further demonstrate that EMB1579 interacts with CUL4 and DDB1, and EMB1579 condensates can recruit and condense MSI4 and DDB1. Furthermore,emb1579phenocopiesmsi4in terms of the level of H3K27 trimethylation onFLC. This allows us to propose that EMB1579 condensates recruit and condense CUL4-DDB1(MSI4)complex, which facilitates the interaction of CUL4-DDB1(MSI4)with CLF-PRC2 and promotes the role of CLF-PRC2 in establishing and/or maintaining the level of H3K27 trimethylation onFLC. Thus, we report a new mechanism for regulating plant gene transcription, mRNA splicing, and growth and development.
学科领域Biochemistry & Molecular Biology ; Biology
DOI10.1371/journal.pbio.3000782
收录类别SCI
语种英语
WOS关键词EPIGENETIC REGULATION ; LIQUID DROPLETS ; FLOWERING TIME ; PROTEIN ; GENE ; EXPRESSION ; TRANSITION ; COMPLEX ; ORGANIZATION ; CUL4-DDB1
WOS研究方向Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
WOS记录号WOS:000554503400001
出版者PUBLIC LIBRARY SCIENCE
文献子类Article
出版地SAN FRANCISCO
EISSN1545-7885
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31471266] ; Beijing Advanced Innovation Center for Structural Biology
作者邮箱sjhuang@tsinghua.edu.cn
作品OA属性gold, Green Published
引用统计
被引频次:24[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/21650
专题中科院北方资源植物重点实验室
作者单位1.Tsinghua Univ, Sch Life Sci, Ctr Plant Biol, Beijing, Peoples R China
2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China
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GB/T 7714
Zhang, Yiling,Li, Zhankun,Chen, Naizhi,et al. Phase separation of Arabidopsis EMB1579 controls transcription, mRNA splicing, and development[J]. PLOS BIOLOGY,2020,18(7).
APA Zhang, Yiling,Li, Zhankun,Chen, Naizhi,Huang, Yao,&Huang, Shanjin.(2020).Phase separation of Arabidopsis EMB1579 controls transcription, mRNA splicing, and development.PLOS BIOLOGY,18(7).
MLA Zhang, Yiling,et al."Phase separation of Arabidopsis EMB1579 controls transcription, mRNA splicing, and development".PLOS BIOLOGY 18.7(2020).
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