Transcriptomic Analysis of the Grapevine LEA Gene Family in Response to Osmotic and Cold Stress Reveals a Key Role for VamDHN3

doi:10.1093/pcp/pcaa004

	Transcriptomic Analysis of the Grapevine LEA Gene Family in Response to Osmotic and Cold Stress Reveals a Key Role for VamDHN3
	Xu, Meilong 1,2; Tong, Qian 1; Wang, Yi 1; Wang, Zemin 1; Xu, Guangzhao 1; Elias, Gathunga Kirabi 1; Li, Shaohua1 ; Liang, Zhenchang 3
	2020
发表期刊	PLANT AND CELL PHYSIOLOGY
ISSN	0032-0781
卷号	61 期号:4 页码:775-786
摘要	Late embryogenesis abundant (LEA) proteins comprise a large family that plays important roles in the regulation of abiotic stress, however, no in-depth analysis of LEA genes has been performed in grapevine to date. In this study, we analyzed a total of 52 putative LEA genes in grapevine at the genomic and transcriptomic level, compiled expression profiles of four selected (V. amurensis) VamLEA genes under cold and osmotic stresses, and studied the potential function of the V. amurensis DEHYDRIN3 (VamDHN3) gene in grapevine callus. The 52 LEA proteins were classified into seven phylogenetic groups. RNA-seq and quantitative real-time PCR results demonstrated that a total of 16 and 23 VamLEA genes were upregulated under cold and osmotic stresses, respectively. In addition, overexpression of VamDHN3 enhanced the stability of the cell membrane in grapevine callus, suggesting that VamDHN3 is involved in osmotic regulation. These results provide fundamental knowledge for the further analysis of the biological roles of grapevine LEA genes in adaption to abiotic stress.
关键词	Cold and osmotic stresses Grapevine LEA genes Overexpression of VamDHN3 gene Phylogenetic analysis Transcriptomic analysis
学科领域	Plant Sciences ; Cell Biology
DOI	10.1093/pcp/pcaa004
收录类别	SCI
语种	英语
WOS关键词	LATE-EMBRYOGENESIS-ABUNDANT ; HABROCHAITES ENHANCES TOLERANCE ; GENOME-WIDE IDENTIFICATION ; DEHYDRIN GENE ; EXPRESSION ANALYSIS ; ESCHERICHIA-COLI ; OXIDATIVE STRESS ; ABIOTIC STRESSES ; PROTEINS ; OVEREXPRESSION
WOS研究方向	Plant Sciences ; Cell Biology
WOS记录号	WOS:000553476300011
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1471-9053
资助机构	Grape breeding project of Ningxia, China [NXNYYZ201502] ; major science and technology program of Ningxia Hui Autonomous region, China [2016BZ06]
作者邮箱	shhli@ibcas.ac.cn ; zl249@ibcas.ac.cn
引用统计	被引频次：27[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21653
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, Beijing Key Lab Grape Sci & Enol,CAS Key Lab Plan, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.State Key Lab Seedling Bioengn, Yinchuan 750004, Ningxia, Peoples R China 4.Chinese Acad Sci, Sino Africa Joint Res Ctr, Wuhan 430074, Peoples R China
推荐引用方式 GB/T 7714	Xu, Meilong,Tong, Qian,Wang, Yi,et al. Transcriptomic Analysis of the Grapevine LEA Gene Family in Response to Osmotic and Cold Stress Reveals a Key Role for VamDHN3[J]. PLANT AND CELL PHYSIOLOGY,2020,61(4):775-786.
APA	Xu, Meilong.,Tong, Qian.,Wang, Yi.,Wang, Zemin.,Xu, Guangzhao.,...&Liang, Zhenchang.(2020).Transcriptomic Analysis of the Grapevine LEA Gene Family in Response to Osmotic and Cold Stress Reveals a Key Role for VamDHN3.PLANT AND CELL PHYSIOLOGY,61(4),775-786.
MLA	Xu, Meilong,et al."Transcriptomic Analysis of the Grapevine LEA Gene Family in Response to Osmotic and Cold Stress Reveals a Key Role for VamDHN3".PLANT AND CELL PHYSIOLOGY 61.4(2020):775-786.

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