IB-CAS  > 中科院北方资源植物重点实验室
Transcriptomic Analysis of the Grapevine LEA Gene Family in Response to Osmotic and Cold Stress Reveals a Key Role for VamDHN3
Xu, Meilong1,2; Tong, Qian1; Wang, Yi1; Wang, Zemin1; Xu, Guangzhao1; Elias, Gathunga Kirabi1; Li, Shaohua1; Liang, Zhenchang3
2020
Source PublicationPLANT AND CELL PHYSIOLOGY
ISSN0032-0781
Volume61Issue:4Pages:775-786
AbstractLate embryogenesis abundant (LEA) proteins comprise a large family that plays important roles in the regulation of abiotic stress, however, no in-depth analysis of LEA genes has been performed in grapevine to date. In this study, we analyzed a total of 52 putative LEA genes in grapevine at the genomic and transcriptomic level, compiled expression profiles of four selected (V. amurensis) VamLEA genes under cold and osmotic stresses, and studied the potential function of the V. amurensis DEHYDRIN3 (VamDHN3) gene in grapevine callus. The 52 LEA proteins were classified into seven phylogenetic groups. RNA-seq and quantitative real-time PCR results demonstrated that a total of 16 and 23 VamLEA genes were upregulated under cold and osmotic stresses, respectively. In addition, overexpression of VamDHN3 enhanced the stability of the cell membrane in grapevine callus, suggesting that VamDHN3 is involved in osmotic regulation. These results provide fundamental knowledge for the further analysis of the biological roles of grapevine LEA genes in adaption to abiotic stress.
KeywordCold and osmotic stresses Grapevine LEA genes Overexpression of VamDHN3 gene Phylogenetic analysis Transcriptomic analysis
Subject AreaPlant Sciences ; Cell Biology
DOI10.1093/pcp/pcaa004
Indexed BySCI
Language英语
WOS KeywordLATE-EMBRYOGENESIS-ABUNDANT ; HABROCHAITES ENHANCES TOLERANCE ; GENOME-WIDE IDENTIFICATION ; DEHYDRIN GENE ; EXPRESSION ANALYSIS ; ESCHERICHIA-COLI ; OXIDATIVE STRESS ; ABIOTIC STRESSES ; PROTEINS ; OVEREXPRESSION
WOS Research AreaPlant Sciences ; Cell Biology
WOS IDWOS:000553476300011
PublisherOXFORD UNIV PRESS
SubtypeArticle
Publication PlaceOXFORD
EISSN1471-9053
Funding OrganizationGrape breeding project of Ningxia, China [NXNYYZ201502] ; major science and technology program of Ningxia Hui Autonomous region, China [2016BZ06]
Corresponding Author Emailshhli@ibcas.ac.cn ; zl249@ibcas.ac.cn
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Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ibcas.ac.cn/handle/2S10CLM1/21653
Collection中科院北方资源植物重点实验室
Affiliation1.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, Beijing Key Lab Grape Sci & Enol,CAS Key Lab Plan, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.State Key Lab Seedling Bioengn, Yinchuan 750004, Ningxia, Peoples R China
4.Chinese Acad Sci, Sino Africa Joint Res Ctr, Wuhan 430074, Peoples R China
Recommended Citation
GB/T 7714
Xu, Meilong,Tong, Qian,Wang, Yi,et al. Transcriptomic Analysis of the Grapevine LEA Gene Family in Response to Osmotic and Cold Stress Reveals a Key Role for VamDHN3[J]. PLANT AND CELL PHYSIOLOGY,2020,61(4):775-786.
APA Xu, Meilong.,Tong, Qian.,Wang, Yi.,Wang, Zemin.,Xu, Guangzhao.,...&Liang, Zhenchang.(2020).Transcriptomic Analysis of the Grapevine LEA Gene Family in Response to Osmotic and Cold Stress Reveals a Key Role for VamDHN3.PLANT AND CELL PHYSIOLOGY,61(4),775-786.
MLA Xu, Meilong,et al."Transcriptomic Analysis of the Grapevine LEA Gene Family in Response to Osmotic and Cold Stress Reveals a Key Role for VamDHN3".PLANT AND CELL PHYSIOLOGY 61.4(2020):775-786.
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