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| Functional analysis of a novel C-glycosyltransferase in the orchid Dendrobium catenatum | |
| Ren, Zhiyao; Ji, Xiaoyu1; Jiao, Zhenbin2,3; Luo, Yingyi6; Zhang, Guo-Qiang; Tao, Shengchang7; Lei, Zhouxi; Zhang, Jing; Wang, Yuchen; Liu, Zhong-Jian; Wei, Gang | |
| 2020 | |
| Source Publication | HORTICULTURE RESEARCH
 |
| ISSN | 2662-6810 |
| Volume | 7Issue:1 |
| Abstract | Flavonoids, which are a diverse class of phytonutrients, are used by organisms to respond to nearly all abiotic stresses and are beneficial for human health. Glycosyltransferase, used during the last step of flavonoid biosynthesis, is important in flavonoid enrichment. However, little is known about glycosyltransferase in the orchid Dendrobium catenatum (D. officinale). In this study, we isolated a novel C-glycosyltransferase (designated DcaCGT) from the orchid D. catenatum by identifying and analyzing 82 putative genes in the GT1 family. DcaCGT could specifically catalyze not only di-C-glycosylation but also O-glycosylation. Apart from the normal function of catalyzing 2-hydroxynaringenin and phloretin to the respective di-C-glycosides, DcaCGT also catalyzes apigenin to cosmosiin. Targeted metabolic profiling of the substrates (2-hydroxynaringenin, phloretin, and apigenin) and products (vitexin, isovitexin, vicenin-2, nothofagin, 3',5'-di-C-glucosylphloretin, and cosmosiin) in different tissues showed that vicenin-2 was the most abundant product of this novel enzyme. Cosmosiin was detected in flowers and flower buds. We also established that DcaCGT functions expanded throughout the evolution of D. catenatum. Residual OGT activity may help D. catenatum resist drought stress. Our study illustrates the function, origin, and differentiation of DcaCGT and provides insights into glycosylation and molecular propagation processes, which can be used to improve the production of flavonoids by the cultivated medicinal plant D. catenatum. |
| Subject Area | Plant Sciences ; Genetics & Heredity ; Horticulture |
| DOI | 10.1038/s41438-020-0330-4 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | FAMILY-1 UDP-GLYCOSYLTRANSFERASES ; GENOME-WIDE IDENTIFICATION ; PHYLOGENETIC ANALYSIS ; VICENIN 2 ; FLAVONOIDS ; GLUCOSYLTRANSFERASES ; EXPRESSION ; GLYCOSIDES ; EXTRACT ; FLOWERS |
| WOS Research Area | Plant Sciences ; Genetics & Heredity ; Agriculture |
| WOS ID | WOS:000546976200016 |
| Publisher | NANJING AGRICULTURAL UNIV |
| Subtype | Article |
| Publication Place | NANJING |
| EISSN | 2052-7276 |
| Funding Organization | 2018 Shaoguan City Science and Technology Plan Project: Special Project of Industry-University-Research Cooperation [2018CS11919] ; 2019 Guangdong Province Special Fund for Science and Technology('Big project + task list') Project: Ecological Cultivation and Sustainable Utilization of Danxia Dendrobium officinale, a rare Southern Medicine in Guangdong Province [2019gdskjzxzj-zt3-2] ; Fundamental Research Project of Shenzhen, China [JCYJ20170817105300166] ; National Key Research and Development Program of China [2018YFD1000401] ; Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization Construction Funds [115/118990050, 115/KJG18016A] |
| Corresponding Author Email | zjliu@fafu.edu.cn ; weigang021@outlook.com |
| OA | Green Published, gold |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/21683 |
| Collection | 系统与进化植物学国家重点实验室 |
| Affiliation | 1.Guangzhou Univ Chinese Med, Sch Pharmaceut Sci, Guangzhou 510006, Peoples R China 2.Shantou Univ, Med Coll, Shantou 515041, Peoples R China 3.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Natl Orchid Conservat Ctr China, Shenzhen Key Lab Orchid Conservat & Utilizat, Shenzhen 518114, Peoples R China 6.Orchid Conservat & Res Ctr Shenzhen, Shenzhen 518114, Peoples R China 7.Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Dept Pharm, Guangzhou 510120, Guangdong, Peoples R China 8.Shaoguan Inst Danxia Dendrobium Officinale, Shaoguan 512005, Peoples R China 9.Fujian Agr & Forestry Univ, Coll Landscape Architecture, Key Lab Natl Forestry & Grassland Adm Orchid Cons, Fuzhou 350002, Peoples R China 10.Shaoguan Univ, Henry Fok Coll Biol & Agr, Shaoguan 512005, Peoples R China |
| Recommended Citation GB/T 7714 | Ren, Zhiyao,Ji, Xiaoyu,Jiao, Zhenbin,et al. Functional analysis of a novel C-glycosyltransferase in the orchid Dendrobium catenatum[J]. HORTICULTURE RESEARCH,2020,7(1). |
| APA | Ren, Zhiyao.,Ji, Xiaoyu.,Jiao, Zhenbin.,Luo, Yingyi.,Zhang, Guo-Qiang.,...&Wei, Gang.(2020).Functional analysis of a novel C-glycosyltransferase in the orchid Dendrobium catenatum.HORTICULTURE RESEARCH,7(1). |
| MLA | Ren, Zhiyao,et al."Functional analysis of a novel C-glycosyltransferase in the orchid Dendrobium catenatum".HORTICULTURE RESEARCH 7.1(2020). |
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| Ren-2020-Functional （4710KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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