IB-CAS  > 系统与进化植物学国家重点实验室
Functional analysis of a novel C-glycosyltransferase in the orchid Dendrobium catenatum
Ren, Zhiyao; Ji, Xiaoyu1; Jiao, Zhenbin2,3; Luo, Yingyi6; Zhang, Guo-Qiang; Tao, Shengchang7; Lei, Zhouxi; Zhang, Jing; Wang, Yuchen; Liu, Zhong-Jian; Wei, Gang
2020
Source PublicationHORTICULTURE RESEARCH
ISSN2662-6810
Volume7Issue:1
AbstractFlavonoids, which are a diverse class of phytonutrients, are used by organisms to respond to nearly all abiotic stresses and are beneficial for human health. Glycosyltransferase, used during the last step of flavonoid biosynthesis, is important in flavonoid enrichment. However, little is known about glycosyltransferase in the orchid Dendrobium catenatum (D. officinale). In this study, we isolated a novel C-glycosyltransferase (designated DcaCGT) from the orchid D. catenatum by identifying and analyzing 82 putative genes in the GT1 family. DcaCGT could specifically catalyze not only di-C-glycosylation but also O-glycosylation. Apart from the normal function of catalyzing 2-hydroxynaringenin and phloretin to the respective di-C-glycosides, DcaCGT also catalyzes apigenin to cosmosiin. Targeted metabolic profiling of the substrates (2-hydroxynaringenin, phloretin, and apigenin) and products (vitexin, isovitexin, vicenin-2, nothofagin, 3',5'-di-C-glucosylphloretin, and cosmosiin) in different tissues showed that vicenin-2 was the most abundant product of this novel enzyme. Cosmosiin was detected in flowers and flower buds. We also established that DcaCGT functions expanded throughout the evolution of D. catenatum. Residual OGT activity may help D. catenatum resist drought stress. Our study illustrates the function, origin, and differentiation of DcaCGT and provides insights into glycosylation and molecular propagation processes, which can be used to improve the production of flavonoids by the cultivated medicinal plant D. catenatum.
Subject AreaPlant Sciences ; Genetics & Heredity ; Horticulture
DOI10.1038/s41438-020-0330-4
Indexed BySCI
Language英语
WOS KeywordFAMILY-1 UDP-GLYCOSYLTRANSFERASES ; GENOME-WIDE IDENTIFICATION ; PHYLOGENETIC ANALYSIS ; VICENIN 2 ; FLAVONOIDS ; GLUCOSYLTRANSFERASES ; EXPRESSION ; GLYCOSIDES ; EXTRACT ; FLOWERS
WOS Research AreaPlant Sciences ; Genetics & Heredity ; Agriculture
WOS IDWOS:000546976200016
PublisherNANJING AGRICULTURAL UNIV
SubtypeArticle
Publication PlaceNANJING
EISSN2052-7276
Funding Organization2018 Shaoguan City Science and Technology Plan Project: Special Project of Industry-University-Research Cooperation [2018CS11919] ; 2019 Guangdong Province Special Fund for Science and Technology('Big project + task list') Project: Ecological Cultivation and Sustainable Utilization of Danxia Dendrobium officinale, a rare Southern Medicine in Guangdong Province [2019gdskjzxzj-zt3-2] ; Fundamental Research Project of Shenzhen, China [JCYJ20170817105300166] ; National Key Research and Development Program of China [2018YFD1000401] ; Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization Construction Funds [115/118990050, 115/KJG18016A]
Corresponding Author Emailzjliu@fafu.edu.cn ; weigang021@outlook.com
OAGreen Published, gold
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ibcas.ac.cn/handle/2S10CLM1/21683
Collection系统与进化植物学国家重点实验室
Affiliation1.Guangzhou Univ Chinese Med, Sch Pharmaceut Sci, Guangzhou 510006, Peoples R China
2.Shantou Univ, Med Coll, Shantou 515041, Peoples R China
3.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Natl Orchid Conservat Ctr China, Shenzhen Key Lab Orchid Conservat & Utilizat, Shenzhen 518114, Peoples R China
6.Orchid Conservat & Res Ctr Shenzhen, Shenzhen 518114, Peoples R China
7.Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Dept Pharm, Guangzhou 510120, Guangdong, Peoples R China
8.Shaoguan Inst Danxia Dendrobium Officinale, Shaoguan 512005, Peoples R China
9.Fujian Agr & Forestry Univ, Coll Landscape Architecture, Key Lab Natl Forestry & Grassland Adm Orchid Cons, Fuzhou 350002, Peoples R China
10.Shaoguan Univ, Henry Fok Coll Biol & Agr, Shaoguan 512005, Peoples R China
Recommended Citation
GB/T 7714
Ren, Zhiyao,Ji, Xiaoyu,Jiao, Zhenbin,et al. Functional analysis of a novel C-glycosyltransferase in the orchid Dendrobium catenatum[J]. HORTICULTURE RESEARCH,2020,7(1).
APA Ren, Zhiyao.,Ji, Xiaoyu.,Jiao, Zhenbin.,Luo, Yingyi.,Zhang, Guo-Qiang.,...&Wei, Gang.(2020).Functional analysis of a novel C-glycosyltransferase in the orchid Dendrobium catenatum.HORTICULTURE RESEARCH,7(1).
MLA Ren, Zhiyao,et al."Functional analysis of a novel C-glycosyltransferase in the orchid Dendrobium catenatum".HORTICULTURE RESEARCH 7.1(2020).
Files in This Item:
File Name/Size DocType Version Access License
Ren-2020-Functional (4710KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Ren, Zhiyao]'s Articles
[Ji, Xiaoyu]'s Articles
[Jiao, Zhenbin]'s Articles
Baidu academic
Similar articles in Baidu academic
[Ren, Zhiyao]'s Articles
[Ji, Xiaoyu]'s Articles
[Jiao, Zhenbin]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Ren, Zhiyao]'s Articles
[Ji, Xiaoyu]'s Articles
[Jiao, Zhenbin]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Ren-2020-Functional analysis of a novel C-glyc.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.