Structural basis for plant lutein biosynthesis from alpha-carotene

doi:10.1073/pnas.2001806117

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	Structural basis for plant lutein biosynthesis from alpha-carotene
	Niu, Guoqi 1,4; Guo, Qi 2; Wang, Jia 2; Zhao, Shun 4; He, Yikun 1; Liu, Lin 3
	2020
发表期刊	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN	0027-8424
卷号	117 期号:25 页码:14150-14157
摘要	Two cytochrome P450 enzymes, CYP97A3 and CYP97C1, catalyze hydroxylations of the beta- and epsilon-rings of alpha-carotene to produce lu- tein. Chirality is introduced at the C-3 atom of both rings, and the reactions are both pro-3 R-stereospecific. We determined the crys- tal structures of CYP97A3 in substrate -free and complex forms with a nonnatural substrate and the structure of CYP97C1 in a detergent -bound form. The structures of CYP97A3 in different states show the substrate channel and the structure of CYP97C1 bound with octylthioglucoside confirms the binding site for the carotenoid substrate. Biochemical assays confirm that the ferredoxin- NADP + reductase (FNR) -ferredoxin pair is used as the redox part- ner. Details of the pro -3 R stereospecificity are revealed in the retinal -bound CYP97A3 structure. Further analysis indicates that the CYP97B clan bears similarity to the beta-ring -specific CYP97A clan. Overall, our research describes the molecular basis for the last steps of lutein biosynthesis.
关键词	xanthophyll P450 hydroxylation chirality stereospecificity
学科领域	Multidisciplinary Sciences
DOI	10.1073/pnas.2001806117
收录类别	SCI
语种	英语
WOS关键词	CYTOCHROME P450 REDUCTASE ; ARABIDOPSIS-THALIANA ; STRUCTURE REFINEMENT ; CRYSTAL-STRUCTURE ; PROVIDES INSIGHT ; BETA-RING ; HYDROXYLASES ; BINDING ; METABOLISM ; EXPRESSION
WOS研究方向	Science & Technology - Other Topics
WOS记录号	WOS:000546772500017
出版者	NATL ACAD SCIENCES
文献子类	Article
出版地	WASHINGTON
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31670794, 31530006] ; National Key R&D Program of China [2017YFA0503703] ; Chinese Academy of SciencesChinese Academy of Sciences [XDB17030100] ; Chang Jiang Scholars ProgramProgram for Changjiang Scholars & Innovative Research Team in University (PCSIRT) [Q2017241] ; Anhui Provincial Wanjiang Scholars Program
作者邮箱	yhe@cnu.edu.cn ; liulin@ahu.edu.cn
作品OA属性	Bronze, Green Published
引用统计	被引频次：31[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21688
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Chinese Acad Sci Key Lab Photobiol, Beijing 100093, Peoples R China 2.Capital Normal Univ, Coll Life Sci, Beijing 100048, Peoples R China 3.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China 4.Anhui Univ, Sch Life Sci, Hefei 230601, Anhui, Peoples R China 5.Mem Sloan Kettering Canc Ctr, Struct Biol Program, New York, NY 10065 USA
推荐引用方式 GB/T 7714	Niu, Guoqi,Guo, Qi,Wang, Jia,et al. Structural basis for plant lutein biosynthesis from alpha-carotene[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2020,117(25):14150-14157.
APA	Niu, Guoqi,Guo, Qi,Wang, Jia,Zhao, Shun,He, Yikun,&Liu, Lin.(2020).Structural basis for plant lutein biosynthesis from alpha-carotene.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,117(25),14150-14157.
MLA	Niu, Guoqi,et al."Structural basis for plant lutein biosynthesis from alpha-carotene".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 117.25(2020):14150-14157.

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