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| Stacking triple genes increased proanthocyanidins level inArabidopsis thaliana | |
| Wei, Jiebing1,2,3; Yang, Junfeng1,2,3; Jiang, Wenbo2; Pang, Yongzhen2 | |
| 2020 | |
| 发表期刊 | PLOS ONE
 |
| ISSN | 1932-6203 |
| 卷号 | 15期号:6 |
| 摘要 | Anthocyanins and proanthocyanidins are two important plant secondary metabolites, and they contribute to plant survival and human health. In particular, proanthocyanidins could also prevent ruminants from the damage of pasture bloat. However, the improvement of proanthocyanidins content remain unsatisfied. In this study, we attempted to improve proanthocyanidins level by gene stacking inArabidopsis thalianaas prove-of-concept. Two proanthocyanidin pathway genes from tea plant,CsF3'5'HandCsANR2, were co-expressed in the wild type andPAP1over-expressionArabidopsis. Over-expression ofCsF3'5'Hslightly affected anthocyanins level in leaves and proanthocyanidins in mature seed when expressed alone in thepap1-Dline. Over-expression ofCsANR2led to an obvious decrease in anthocyanins in leaves of both wild type andpap1-Dlines, but increase in proanthocyanidin level in mature seeds. Over-expression ofCsANR2inpap1-Dlines lead to production of DMACA-reactive soluble proanthocyanidins in leaves, but not in wild type orpap1-D lines. Anthocyanins level was decreased in the leaves ofCsF3'5'H,CsANR2andpap1-Dco-expression lines, but proanthocyanidins were increased remarkably in both leaves and mature seeds in the co-expression line. It is concluded that co-expression ofCsANR2andPAP1inArabidopsisproduce soluble proanthocyanidins in leaves, and co-expression ofCsF3'5'H,CsANR2andPAP1lead to a significant increase in proanthocyanidins in mature seeds. The transcript levels of endogenousCHS,DFR,ANSandANRgenes inArabidopsiswere up-regulated in the triple genes co-expression line. Based on these studies, it is possible to develop new plant germplasm with improved proanthocyanidins by co-expressing of multiple genes. |
| 学科领域 | Multidisciplinary Sciences |
| DOI | 10.1371/journal.pone.0234799 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | LEUCOANTHOCYANIDIN REDUCTASE ; CONDENSED TANNINS ; BIOSYNTHESIS ; EXPRESSION ; TEA ; PATHWAYS ; ENZYMES ; PLANTS ; FLUX |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS记录号 | WOS:000545747200001 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 文献子类 | Article |
| 出版地 | SAN FRANCISCO |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31870281] |
| 作者邮箱 | jiangwenbo@caas.cn ; pangyongzhen@caas.cn |
| 作品OA属性 | Green Published, gold |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/21695 |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing, Peoples R China 3.Chinese Acad Agr Sci, Inst Anim Sci, Beijing, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Jiebing,Yang, Junfeng,Jiang, Wenbo,et al. Stacking triple genes increased proanthocyanidins level inArabidopsis thaliana[J]. PLOS ONE,2020,15(6). |
| APA | Wei, Jiebing,Yang, Junfeng,Jiang, Wenbo,&Pang, Yongzhen.(2020).Stacking triple genes increased proanthocyanidins level inArabidopsis thaliana.PLOS ONE,15(6). |
| MLA | Wei, Jiebing,et al."Stacking triple genes increased proanthocyanidins level inArabidopsis thaliana".PLOS ONE 15.6(2020). |
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| Wei-2020-Stacking tr（2407KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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