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| Mutagenesis of a Quintuple Mutant Impaired in Environmental Responses Reveals Roles for CHROMATIN REMODELING4 in the Arabidopsis Floral Transition | |
| Sang, Qing; Pajoro, Alice; Sun, Hequan; Song, Baoxing; Yang, Xia1; Stolze, Sara C.; Andres, Fernando2; Schneeberger, Korbinian; Nakagami, Hirofumi; Coupland, George | |
| 2020 | |
| 发表期刊 | PLANT CELL
 |
| ISSN | 1040-4651 |
| 卷号 | 32期号:5页码:1479-1500 |
| 摘要 | Several pathways conferring environmental flowering responses in Arabidopsis (Arabidopsis thaliana) converge on developmental processes that mediate the floral transition in the shoot apical meristem. Many characterized mutations disrupt these environmental responses, but downstream developmental processes have been more refractory to mutagenesis. Here, we constructed a quintuple mutant impaired in several environmental pathways and showed that it possesses severely reduced flowering responses to changes in photoperiod and ambient temperature. RNA-sequencing (RNA-seq) analysis of the quintuple mutant showed that the expression of genes encoding gibberellin biosynthesis enzymes and transcription factors involved in the age pathway correlates with flowering. Mutagenesis of the quintuple mutant generated two late-flowering mutants, quintuple ems1 (qem1) and qem2. The mutated genes were identified by isogenic mapping and transgenic complementation. The qem1 mutant is an allele of the gibberellin 20-oxidase gene ga20ox2, confirming the importance of gibberellin for flowering in the absence of environmental responses. By contrast, qem2 is impaired in CHROMATIN REMODELING4 (CHR4), which has not been genetically implicated in floral induction. Using co-immunoprecipitation, RNA-seq, and chromatin immunoprecipitation sequencing, we show that CHR4 interacts with transcription factors involved in floral meristem identity and affects the expression of key floral regulators. Therefore, CHR4 mediates the response to endogenous flowering pathways in the inflorescence meristem to promote floral identity. |
| 学科领域 | Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology |
| DOI | 10.1105/tpc.19.00992 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | CHROMATIN-REMODELING FACTOR ; LATE-FLOWERING MUTANTS ; DOMAIN PROTEIN ; FT PROTEIN ; AMBIENT-TEMPERATURE ; MERISTEM IDENTITY ; SHOOT DEVELOPMENT ; ORGAN IDENTITY ; LIFE-CYCLE ; BOX GENE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology |
| WOS记录号 | WOS:000545741500021 |
| 出版者 | AMER SOC PLANT BIOLOGISTS |
| 文献子类 | Article |
| 出版地 | ROCKVILLE |
| EISSN | 1531-298X |
| 资助机构 | European Molecular Biology OrganizationEuropean Molecular Biology Organization (EMBO) [AFL-2017-74] ; China Scholarship CouncilChina Scholarship Council [201804910196] ; Deutsche Forschungsgemeinschaft under Germany's Excellence StrategyGerman Research Foundation (DFG) [DFGEXC 2048/1, 390686111] ; Max Planck SocietyMax Planck SocietyFoundation CELLEX |
| 作者邮箱 | coupland@mpipz.mpg.de |
| 作品OA属性 | Green Published, Bronze |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/21696 |
| 专题 | 系统与进化植物学国家重点实验室 |
| 作者单位 | 1.Max Planck Inst Plant Breeding Res, D-50829 Cologne, Germany 2.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 3.Univ Montpellier, SupAgro, Ctr Cooperat Int Rech Agron Dev, INRA,Ameliorat Genet & Adaptat Plantes Mediterran, F-34398 Montpellier, France |
| 推荐引用方式 GB/T 7714 | Sang, Qing,Pajoro, Alice,Sun, Hequan,et al. Mutagenesis of a Quintuple Mutant Impaired in Environmental Responses Reveals Roles for CHROMATIN REMODELING4 in the Arabidopsis Floral Transition[J]. PLANT CELL,2020,32(5):1479-1500. |
| APA | Sang, Qing.,Pajoro, Alice.,Sun, Hequan.,Song, Baoxing.,Yang, Xia.,...&Coupland, George.(2020).Mutagenesis of a Quintuple Mutant Impaired in Environmental Responses Reveals Roles for CHROMATIN REMODELING4 in the Arabidopsis Floral Transition.PLANT CELL,32(5),1479-1500. |
| MLA | Sang, Qing,et al."Mutagenesis of a Quintuple Mutant Impaired in Environmental Responses Reveals Roles for CHROMATIN REMODELING4 in the Arabidopsis Floral Transition".PLANT CELL 32.5(2020):1479-1500. |
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| Sang-2020-Mutagenesi（2676KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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