SlREM1 Triggers Cell Death by Activating an Oxidative Burst and Other Regulators(1)

doi:10.1104/pp.20.00120

	SlREM1 Triggers Cell Death by Activating an Oxidative Burst and Other Regulators(1)
	Cai, Jianghua 1; Chen, Tong ; Wang, Ying 1; Qin, Guozheng1 ; Tian, Shiping1
	2020
发表期刊	PLANT PHYSIOLOGY
ISSN	0032-0889
卷号	183 期号:2 页码:717-732
摘要	Programmed cell death (PCD), a highly regulated feature of the plant immune response, involves multiple molecular players. Remorins (REMs) are plant-specific proteins with varied biological functions, but their function in PCD and plant defense remains largely unknown. Here, we report a role for remorin in disease resistance, immune response, and PCD regulation. Overexpression of tomato (Solanum lycopersicum)REMORIN1(SlREM1) increased susceptibility of tomato to the necrotrophic fungusBotrytis cinereaand heterologous expression of this gene triggered cell death inNicotiana benthamianaleaves. Further investigation indicated that amino acids 173 to 187 and phosphorylation of SlREM1 played key roles in SlREM1-triggered cell death. Intriguingly, multiple tomato REMs induced cell death inN.benthamianaleaves. Yeast two-hybrid, split luciferase complementation, and coimmunoprecipitation assays all demonstrated that remorin proteins could form homo- and heterocomplexes. Using isobaric tags for relative and absolute quantitative proteomics, we identified that some proteins related to cell death regulation, as well asN.benthamianaRESPIRATORY BURST OXIDASE HOMOLOG B (which is essential for reactive oxygen species production), were notably upregulated inSlREM1-expressing leaves. Heterologous expression ofSlREM1increased reactive oxygen species accumulation and triggered other cell death regulators. Our findings indicate that SlREM1 is a positive regulator of plant cell death and provide clues for understanding the PCD molecular regulatory network in plants. A tomato remorin protein causes cell death by increasing the accumulation of reactive oxygen species and other defense factors.
学科领域	Plant Sciences
DOI	10.1104/pp.20.00120
收录类别	SCI
语种	英语
WOS关键词	PLASMA-MEMBRANE LOCALIZATION ; E3 UBIQUITIN LIGASE ; TRANSCRIPTION FACTOR ; HYPERSENSITIVE RESPONSE ; PROTEIN-PHOSPHORYLATION ; POSITIVE REGULATOR ; POLLEN DEVELOPMENT ; FRUIT SENESCENCE ; DEFENSE RESPONSE ; BOTRYTIS-CINEREA
WOS研究方向	Plant Sciences
WOS记录号	WOS:000541865100037
出版者	AMER SOC PLANT BIOLOGISTS
文献子类	Article
出版地	ROCKVILLE
EISSN	1532-2548
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31930086, 31530057, 31672210]
作者邮箱	tsp@ibcas.ac.cn
作品OA属性	Green Published, Bronze
引用统计	被引频次：30[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21710
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Cai, Jianghua,Chen, Tong,Wang, Ying,et al. SlREM1 Triggers Cell Death by Activating an Oxidative Burst and Other Regulators(1)[J]. PLANT PHYSIOLOGY,2020,183(2):717-732.
APA	Cai, Jianghua,Chen, Tong,Wang, Ying,Qin, Guozheng,&Tian, Shiping.(2020).SlREM1 Triggers Cell Death by Activating an Oxidative Burst and Other Regulators(1).PLANT PHYSIOLOGY,183(2),717-732.
MLA	Cai, Jianghua,et al."SlREM1 Triggers Cell Death by Activating an Oxidative Burst and Other Regulators(1)".PLANT PHYSIOLOGY 183.2(2020):717-732.

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