LcMYB4, an unknown function transcription factor gene from sheepgrass, as a positive regulator of chilling and freezing tolerance in transgenic Arabidopsis

doi:10.1186/s12870-020-02427-y

	LcMYB4, an unknown function transcription factor gene from sheepgrass, as a positive regulator of chilling and freezing tolerance in transgenic Arabidopsis
	Li, Xiaoxia ; Jia, Junting 1; Zhao, Pincang 2; Guo, Xiufang ; Chen, Shuangyan ; Qi, Dongmei ; Cheng, Liqin ; Liu, Gongshe
	2020
发表期刊	BMC PLANT BIOLOGY
ISSN	1471-2229
卷号	20 期号:1
摘要	BackgroundSheepgrass (Leymus chinensis (Trin.) Tzvel) is a perennial forage grass that can survive extreme freezing winters (-47.5 degrees C) in China. In this study, we isolated an unknown function MYB transcription factor gene, LcMYB4, from sheepgrass. However, the function of LcMYB4 and its homologous genes has not been studied in other plants.ResultsThe expression of the LcMYB4 gene was upregulated in response to cold induction, and the LcMYB4 fusion protein was localized in the nucleus, with transcriptional activation activity. Biological function analysis showed that compared with WT plants, LcMYB4-overexpressing Arabidopsis presented significantly increased chilling and freezing tolerance as evidenced by increased germination rate, survival rate, and seed setting rate under conditions of low temperature stress. Furthermore, LcMYB4-overexpressing plants showed increased soluble sugar content, leaf chlorophyll content and superoxide dismutase activity but decreased malondialdehyde (MDA) under chilling stress. Moreover, the expression of the CBF1, KIN1, KIN2 and RCI2A genes were significantly upregulated in transgenic plants with chilling treatment. These results suggest that LcMYB4 overexpression increased the soluble sugar content and cold-inducible gene expression and alleviated oxidative damage and membrane damage, resulting in enhanced cold resistance in transgenic plants. Interestingly, our results showed that the LcMYB4 protein interacts with fructose-1,6-bisphosphate aldolase protein1 (LcFBA1) and that the expression of the LcFBA1 gene was also upregulated during cold induction in sheepgrass, similar to LcMYB4.ConclusionOur findings suggest that LcMYB4 encodes MYB transcription factor that plays a positive regulatory role in cold stress.
关键词	Sheepgrass MYB transcriptional factor Freezing tolerance Chilling stress Transgenic
学科领域	Plant Sciences
DOI	10.1186/s12870-020-02427-y
收录类别	SCI
语种	英语
WOS关键词	SALT-INDUCED GENE ; STRESS TOLERANCE ; ABIOTIC STRESS ; LOW-TEMPERATURE ; MYB GENE ; DEHYDRATION TOLERANCE ; INCREASES TOLERANCE ; SIGNAL-TRANSDUCTION ; NEGATIVE REGULATOR ; ENHANCED TOLERANCE
WOS研究方向	Plant Sciences
WOS记录号	WOS:000538035600002
出版者	BMC
文献子类	Article
出版地	LONDON
资助机构	Science and Technology Major Project of Inner Mongolia Autonomous Region of China ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31601996] ; National Key Research and Development Program of China [2018YFD1001000, 2016YFC0500700] ; Chinese Academy of SciencesChinese Academy of Sciences [2019SYHZ0035] ; Kulun Banner Science and Technology Poverty Alleviation Project of Chinese Academy of Sciences ; Jilin Province [2019SYHZ0035]
作者邮箱	lqcheng@ibcas.ac.cn ; liugs@ibcas.ac.cn
作品OA属性	Green Published, gold
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21732
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 2.Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangdong Prov Key Lab Crop Germplasm Resources P, Guangzhou, Peoples R China 3.Hebei Univ Econ & Business, Coll Management Sci & Engn, Shijiazhuang, Hebei, Peoples R China
推荐引用方式 GB/T 7714	Li, Xiaoxia,Jia, Junting,Zhao, Pincang,et al. LcMYB4, an unknown function transcription factor gene from sheepgrass, as a positive regulator of chilling and freezing tolerance in transgenic Arabidopsis[J]. BMC PLANT BIOLOGY,2020,20(1).
APA	Li, Xiaoxia.,Jia, Junting.,Zhao, Pincang.,Guo, Xiufang.,Chen, Shuangyan.,...&Liu, Gongshe.(2020).LcMYB4, an unknown function transcription factor gene from sheepgrass, as a positive regulator of chilling and freezing tolerance in transgenic Arabidopsis.BMC PLANT BIOLOGY,20(1).
MLA	Li, Xiaoxia,et al."LcMYB4, an unknown function transcription factor gene from sheepgrass, as a positive regulator of chilling and freezing tolerance in transgenic Arabidopsis".BMC PLANT BIOLOGY 20.1(2020).

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