Reversible SUMOylation of FHY1 Regulates Phytochrome A Signaling in Arabidopsis

doi:10.1016/j.molp.2020.04.002

	Reversible SUMOylation of FHY1 Regulates Phytochrome A Signaling in Arabidopsis
	Qu, Gao-Ping ; Li, Hong 2; Lin, Xiao-Li ; Kong, Xiangxiong ; Hu, Zi-Liang ; Jin, Yin Hua ; Liu, Yu 1; Song, Hang-Lin ; Kim, Dae Heon 4; Lin, Rongcheng5 ; Li, Jigang 2; Jin, Jing Bo
	2020
发表期刊	MOLECULAR PLANT
ISSN	1674-2052
卷号	13 期号:6 页码:879-893
摘要	In response to far-red light (FR), FAR-RED ELONGATED HYPOCOTYL 1 (FHY1) transports the photoactivated phytochrome A (phyA), the primary FR photoreceptor, into the nucleus, where it initiates FR signaling in plants. Light promotes the 26S proteasome-mediated degradation of FHY1, which desensitizes FR signaling, but the underlying regulatory mechanism remains largely unknown. Here, we show that reversible SUMOylation of FHY1 tightly regulates this process. Lysine K32 (K32) and K103 are major SUMOylation sites of FHY1. We found that FR exposure promotes the SUMOylation of FHY1, which accelerates its degradation. Furthermore, we discovered that ARABIDOPSIS SUMO PROTEASE 1 (ASP1) interacts with FHY1 in the nucleus under FR and facilitates its deSUMOylation. FHY1 was strongly SUMOylated and its protein level was decreased in the asp1-1 loss-of-function mutant compared with that in the wild type under FR. Consistently, asp1-1 seedlings exhibited a decreased sensitivity to FR, suggesting that ASP1 plays an important role in the maintenance of proper FHY1 levels under FR. Genetic analysis further revealed that ASP1 regulates FR signaling through an FHY1- and phyA-dependent pathway. Interestingly, We found that continuous FR inhibits ASP1 accumulation, perhaps contributing to the desensitization of FR signaling. Taken together, these results indicate that FR-induced SUMOylation and ASP1-dependent deSUMOylation of FHY1 represent a key regulatory mechanism that fine-tunes FR signaling.
关键词	Arabidopsis photomorphogenesis FHY1 SUMO ASP1
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
DOI	10.1016/j.molp.2020.04.002
收录类别	SCI
语种	英语
WOS关键词	RED ELONGATED HYPOCOTYL1 ; LIGHT-INDUCED PHOSPHORYLATION ; SUMO E3 LIGASE ; NUCLEAR ACCUMULATION ; DEGRADATION ; PROTEINS ; PHOTOMORPHOGENESIS ; DESUMOYLATION ; MODULATION ; INHIBITION
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
WOS记录号	WOS:000537943400012
出版者	CELL PRESS
文献子类	Article
出版地	CAMBRIDGE
EISSN	1752-9867
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31670186, 31870238] ; Chinese Academy of SciencesChinese Academy of Sciences [ZDRW-ZS-2019-2-0101, KFJ-STS-ZDTP-076-1] ; Chinese Academy of Sciences (The Innovative Academy of Seed Design)
作者邮箱	jinjb@ibcas.ac.cn
作品OA属性	Bronze
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21733
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing 100193, Peoples R China 4.Yanbian Acad Agr Sci, Yanji 133001, Peoples R China 5.Sunchon Natl Univ, Dept Biol, Sunchon 57922, South Korea 6.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Qu, Gao-Ping,Li, Hong,Lin, Xiao-Li,et al. Reversible SUMOylation of FHY1 Regulates Phytochrome A Signaling in Arabidopsis[J]. MOLECULAR PLANT,2020,13(6):879-893.
APA	Qu, Gao-Ping.,Li, Hong.,Lin, Xiao-Li.,Kong, Xiangxiong.,Hu, Zi-Liang.,...&Jin, Jing Bo.(2020).Reversible SUMOylation of FHY1 Regulates Phytochrome A Signaling in Arabidopsis.MOLECULAR PLANT,13(6),879-893.
MLA	Qu, Gao-Ping,et al."Reversible SUMOylation of FHY1 Regulates Phytochrome A Signaling in Arabidopsis".MOLECULAR PLANT 13.6(2020):879-893.

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