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Ribonucleoside Diphosphate Reductase Plays an Important Role in Patulin Degradation by Enterobacter cloacae subsp. dissolvens | |
Xing, Mengyang1; Li, Boqiang; Chen, Yong; Tian, Shiping1 | |
2020 | |
发表期刊 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY |
ISSN | 0021-8561 |
卷号 | 68期号:18页码:5232-5240 |
摘要 | Patulin contamination is a worldwide concern due to its significant impact on human health. Several yeast strains have been screened for patulin biodegradation; however, little information is available on bacterial strains and their mechanism of degradation. In the present study, we isolated a bacterial strain TT-09 and identified it as Enterobacter cloacae subsp. dissolvens based on the BioLog system and 16S rDNA phylogenetic analysis. The strain was demonstrated to be able to transform patulin into E-ascladiol. Isobaric tags for relative and absolute quantitation and reverse transcription quantitative polymerase chain reaction analyses provided evidence that ribonucleoside diphosphate reductase (NrdA), an important enzyme involved in DNA biosynthesis, plays a crucial role in patulin degradation. Deletion of nrdA resulted in a total loss in the ability to degrade patulin in TT-09. These results indicate a new function for NrdA in mycotoxin biodegradation. The present study provides evidence for understanding a new mechanism of patulin degradation and information that can be used to develop new approaches for managing patulin contamination. |
关键词 | patulin fruit-derived food mycotoxin contamination biodegradation ribonucleoside diphosphate reductase Enterobacter cloacae |
学科领域 | Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology |
DOI | 10.1021/acs.jafc.0c01613 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | ALKYL HYDROPEROXIDE REDUCTASE ; MYCOTOXIN PATULIN ; ESCHERICHIA-COLI ; DETOXIFICATION ; BIOTRANSFORMATION ; IDENTIFICATION ; RHIZOSPHERE ; EXPRESSION ; MECHANISM ; NITROGEN |
WOS研究方向 | Agriculture ; Chemistry ; Food Science & Technology |
WOS记录号 | WOS:000535173900022 |
出版者 | AMER CHEMICAL SOC |
文献子类 | Article |
出版地 | WASHINGTON |
EISSN | 1520-5118 |
资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31930086, 31722043] ; National Key R&D Program of China [2016YFD0400902] ; Youth Innovation Promotion Association, CAS [Y201919] |
作者邮箱 | tsp@ibcas.ac.cn |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/21753 |
专题 | 中科院北方资源植物重点实验室 |
作者单位 | 1.Inst Bot, Chinese Acad Sci, Innovat Acad Seed Design, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Xing, Mengyang,Li, Boqiang,Chen, Yong,et al. Ribonucleoside Diphosphate Reductase Plays an Important Role in Patulin Degradation by Enterobacter cloacae subsp. dissolvens[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2020,68(18):5232-5240. |
APA | Xing, Mengyang,Li, Boqiang,Chen, Yong,&Tian, Shiping.(2020).Ribonucleoside Diphosphate Reductase Plays an Important Role in Patulin Degradation by Enterobacter cloacae subsp. dissolvens.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,68(18),5232-5240. |
MLA | Xing, Mengyang,et al."Ribonucleoside Diphosphate Reductase Plays an Important Role in Patulin Degradation by Enterobacter cloacae subsp. dissolvens".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 68.18(2020):5232-5240. |
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S00218561_S002185612(2121KB) | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 |
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