Identification of the target genes of AqAPETALA3-3 (AqAP3-3) in Aquilegia coerulea (Ranunculaceae) helps understand the molecular bases of the conserved and nonconserved features of petals

doi:10.1111/nph.16601

	Identification of the target genes of AqAPETALA3-3 (AqAP3-3) in Aquilegia coerulea (Ranunculaceae) helps understand the molecular bases of the conserved and nonconserved features of petals
	Jiang, Yongchao 1; Wang, Meimei 1; Zhang, Rui ; Xie, Jinghe 1; Duan, Xiaoshan ; Shan, Hongyan ; Xu, Guixia; Kong, Hongzhi1
	2020
发表期刊	NEW PHYTOLOGIST
ISSN	0028-646X
卷号	227 期号:4 页码:1235-1248
摘要	Identification and comparison of the conserved and variable downstream genes of floral organ identity regulators are critical to understanding the mechanisms underlying the commonalities and peculiarities of floral organs. Yet, because of the lack of studies in nonmodel species, a general picture of the regulatory evolution between floral organ identity genes and their targets is still lacking. Here, by conducting extensive chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq), electrophoretic mobility shift assay and bioinformatic analyses, we identify and predict the target genes of a petal identity gene, AqAPETALA3-3 (AqAP3-3), in Aquilegia coerulea (Ranunculaceae) and compare them with those of its counterpart in Arabidopsis thaliana, AP3. In total, 7049 direct target genes are identified for AqAP3-3, of which 2394 are highly confident and 1085 are shared with AP3. Gene Ontology enrichment analyses further indicate that conserved targets are largely involved in the formation of identity-related features, whereas nonconserved targets are mostly required for the formation of species-specific features. These results not only help understand the molecular bases of the conserved and nonconserved features of petals, but also pave the way to studying the regulatory evolution between floral organ identity genes and their targets.
关键词	Aquilegia binding sites ChIP-seq floral organ identity genes petals target genes
学科领域	Plant Sciences
DOI	10.1111/nph.16601
收录类别	SCI
语种	英语
WOS关键词	MADS-BOX GENES ; FLORAL ORGAN IDENTITY ; TRANSCRIPTION FACTOR ; CHIP-SEQ ; ARABIDOPSIS PETAL ; HOMEOBOX GENES ; DNA-BINDING ; EVOLUTION ; APETALA3 ; PROTEIN
WOS研究方向	Plant Sciences
WOS记录号	WOS:000533141000001
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1469-8137
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31930008] ; Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDB27010304]
作者邮箱	xuguixia1982@ibcas.ac.cn ; hzkong@ibcas.ac.cn
作品OA属性	Bronze
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21761
专题	系统与进化植物学国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, CAS Ctr Excellence Mol Plant Sci, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Yongchao,Wang, Meimei,Zhang, Rui,et al. Identification of the target genes of AqAPETALA3-3 (AqAP3-3) in Aquilegia coerulea (Ranunculaceae) helps understand the molecular bases of the conserved and nonconserved features of petals[J]. NEW PHYTOLOGIST,2020,227(4):1235-1248.
APA	Jiang, Yongchao.,Wang, Meimei.,Zhang, Rui.,Xie, Jinghe.,Duan, Xiaoshan.,...&Kong, Hongzhi.(2020).Identification of the target genes of AqAPETALA3-3 (AqAP3-3) in Aquilegia coerulea (Ranunculaceae) helps understand the molecular bases of the conserved and nonconserved features of petals.NEW PHYTOLOGIST,227(4),1235-1248.
MLA	Jiang, Yongchao,et al."Identification of the target genes of AqAPETALA3-3 (AqAP3-3) in Aquilegia coerulea (Ranunculaceae) helps understand the molecular bases of the conserved and nonconserved features of petals".NEW PHYTOLOGIST 227.4(2020):1235-1248.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Jiang-2020-Identific（1459KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文