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| Pentatricopeptide repeat protein PHOTOSYSTEM I BIOGENESIS FACTOR2 is required for splicing of ycf3 | |
| Wang, Xuemei1; Yang, Zhipan1; Zhang, Yi2; Zhou, Wen1; Zhang, Aihong2; Lu, Congming2 | |
| 2020 | |
| 发表期刊 | JOURNAL OF INTEGRATIVE PLANT BIOLOGY
 |
| ISSN | 1672-9072 |
| 卷号 | 62期号:11页码:1741-1761 |
| 摘要 | To gain a better understanding of the molecular mechanisms of photosystem I (PSI) biogenesis, we characterized the Arabidopsis thaliana photosystem I biogenesis factor 2 (pbf2) mutant, which lacks PSI complex. PBF2 encodes a P-class pentatricopeptide repeat (PPR) protein. In the pbf2 mutants, we observed a striking decrease in the transcript level of only one gene, the chloroplast gene ycf3, which is essential for PSI assembly. Further analysis of ycf3 transcripts showed that PBF2 is specifically required for the splicing of ycf3 intron 1. Computational prediction of binding sequences and electrophoretic mobility shift assays reveal that PBF2 specifically binds to a sequence in ycf3 intron 1. Moreover, we found that PBF2 interacted with two general factors for group II intron splicing CHLOROPLAST RNA SPLICING2-ASSOCIATED FACTOR1 (CAF1) and CAF2, and facilitated the association of these two factors with ycf3 intron 1. Our results suggest that PBF2 is specifically required for the splicing of ycf3 intron 1 through cooperating with CAF1 and CAF2. Our results also suggest that additional proteins are required to contribute to the specificity of CAF-dependent group II intron splicing. |
| 关键词 | chloroplast group II intron P-class pentatricopeptide repeat protein splicing ycf3 intron 1 |
| 学科领域 | Biochemistry & Molecular Biology ; Plant Sciences |
| DOI | 10.1111/jipb.12936 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | STRUCTURAL BASIS ; RNA-BINDING ; ARABIDOPSIS ; INTRON ; TOLERANCE ; PHOTOSYNTHESIS ; BIOSYNTHESIS ; RESPONSES ; PROMOTES ; COMPLEX |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS记录号 | WOS:000532713700001 |
| 出版者 | WILEY |
| 文献子类 | Article |
| 出版地 | HOBOKEN |
| EISSN | 1744-7909 |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31730102, 31401035] ; Key Research Plan of Frontier Sciences of the Chinese Academy of Sciences [QYZDJ-SSW-SMC003] |
| 作者邮箱 | cmlu@sdau.edu.cn |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/21763 |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China 3.Shandong Agr Univ, State Key Lab Crop Biol, Coll Life Sci, Tai An 271018, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xuemei,Yang, Zhipan,Zhang, Yi,et al. Pentatricopeptide repeat protein PHOTOSYSTEM I BIOGENESIS FACTOR2 is required for splicing of ycf3[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2020,62(11):1741-1761. |
| APA | Wang, Xuemei,Yang, Zhipan,Zhang, Yi,Zhou, Wen,Zhang, Aihong,&Lu, Congming.(2020).Pentatricopeptide repeat protein PHOTOSYSTEM I BIOGENESIS FACTOR2 is required for splicing of ycf3.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,62(11),1741-1761. |
| MLA | Wang, Xuemei,et al."Pentatricopeptide repeat protein PHOTOSYSTEM I BIOGENESIS FACTOR2 is required for splicing of ycf3".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 62.11(2020):1741-1761. |
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| Wang-2020-Pentatrico（4076KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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