Lutein-mediated photoprotection of photosynthetic machinery in Arabidopsis thaliana exposed to chronic low ultraviolet-B radiation

doi:10.1016/j.jplph.2020.153160

	Lutein-mediated photoprotection of photosynthetic machinery in Arabidopsis thaliana exposed to chronic low ultraviolet-B radiation
	Biswas, Dilip K.; Ma, Bao-Luo ; Xu, Hong 2; Li, Yonggeng 2; Jiang, Gaoming2
	2020
发表期刊	JOURNAL OF PLANT PHYSIOLOGY
ISSN	0176-1617
卷号	248
摘要	Ecologically relevant low UV-B is reported to alter reactive oxygen species metabolism and anti-oxidative systems through an up-regulation of enzymes of the phenylpropanoid pathway. However, little is known about low UV-B-induced changes in carotenoid profile and their impacts on light harvesting and photoprotection of photosystem II (PSII) in plants. We investigated carotenoids profile, chlorophyll pigments, phenolics, photosynthetic efficiency and growth in Arabidopsis thaliana (Col-0) plants grown under photosynthetically active radiation (PAR), PAR+ ultraviolet (UV)-A and PAR+UV-A+B regimes for 10 days in order to assess plant acclimation to low UV-B radiation. A chlorophyll fluorescence assay was used to examine UV-B tolerance in plants further exposed to acute high UV-B for 4 and 6 h following a 10-day growth under different PAR and UV regimes. We found that both PAR+ UV-A and PAR+UV-A+B regimes had no negative effect on quantum efficiency, electron transport rate, rosette diameter, relative growth rate and shoot dry weight of plants. Chronic PAR+ UV-A regime considerably (P < 0.05) increased violaxanthin (26 %) and neoxanthin (92 %) content in plants. Plant exposure to chronic PAR+UV-A+B significantly (P < 0.05) increased violaxanthin (48 %), neoxanthin (63 %), lutein (33 %), 9-cis beta-carotene (28 %), total beta-carotene (29 %) and total phenolics (108 %). The maximum photochemical efficiency (F-v/F-m) in leaves was found to be positively correlated with total phenolics (rho = 0.81 and rho = 0.91, P < 0.05 for 4 and 6 h, respectively) and non-photochemical quenching (q(N)) (rho = 0.81 and rho = 0.84, P < 0.05 for 4 and 6 h, respectively) in plants exposed to acute high UV-B for 4 and 6 h following a 10-day growth under chronic PAR+UV-A+B. There was also a significant positive correlation (rho = 0.93, P < 0.01) between q(N) and lutein content in the plants exposed to acute high UV-B stress for 4 h following plant exposure to chronic PAR+UV-A+B. The findings from our study indicate that plants grown under chronic PAR+UV-A+B displayed higher photoprotection of PSII against acute high UV-B stress than those grown under PAR and PAR+ UV-A regimes. An induction of phenolics and lutein-mediated development of q(N) were involved in the photoprotection of PSII against UV-B-induced oxidative stress.
关键词	Arabidopsis thaliana Carotenoids Lutein Phenolics Photoprotection Chl a fluorescence UV-B acclimation UV-B tolerance
学科领域	Plant Sciences
DOI	10.1016/j.jplph.2020.153160
收录类别	SCI
语种	英语
WOS关键词	UV-B ; XANTHOPHYLL CYCLE ; CHLOROPHYLL FLUORESCENCE ; OZONE DEPLETION ; GENE-EXPRESSION ; WINTER-WHEAT ; PLANT STRESS ; PROTECTION ; CAROTENOIDS ; CHLOROPLASTS
WOS研究方向	Plant Sciences
WOS记录号	WOS:000530650800002
出版者	ELSEVIER GMBH
文献子类	Article
出版地	MUNICH
EISSN	1618-1328
资助机构	Irish Research Council for Science, Engineering & Technology (IRCSET embark)Irish Research Council for Science, Engineering and Technology ; AAFC Project [J-001959]
作者邮箱	dkb283@mail.usask.ca
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21770
专题	植被与环境变化国家重点实验室
作者单位	1.[Biswas, Dilip K.] Univ Coll Cork, Dept Zool Ecol & Plant Sci, Cork, Ireland 2.Agr & Agri Food Canada, Ottawa Res & Dev Ctr, Ottawa, ON K1A 0C6, Canada 3.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China 4.Biswas, Dilip K.] Univ Saskatchewan, Dept Plant Sci, Saskatoon, SK, Canada
推荐引用方式 GB/T 7714	Biswas, Dilip K.,Ma, Bao-Luo,Xu, Hong,et al. Lutein-mediated photoprotection of photosynthetic machinery in Arabidopsis thaliana exposed to chronic low ultraviolet-B radiation[J]. JOURNAL OF PLANT PHYSIOLOGY,2020,248.
APA	Biswas, Dilip K.,Ma, Bao-Luo,Xu, Hong,Li, Yonggeng,&Jiang, Gaoming.(2020).Lutein-mediated photoprotection of photosynthetic machinery in Arabidopsis thaliana exposed to chronic low ultraviolet-B radiation.JOURNAL OF PLANT PHYSIOLOGY,248.
MLA	Biswas, Dilip K.,et al."Lutein-mediated photoprotection of photosynthetic machinery in Arabidopsis thaliana exposed to chronic low ultraviolet-B radiation".JOURNAL OF PLANT PHYSIOLOGY 248(2020).

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