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A comprehensive analysis of the lysine acetylome reveals diverse functions of acetylated proteins during de -etiolation in Zea mays
Yan, Zhen; Shen, Zhuo2; Gao, Zhi-Fang; Chao, Qing; Qian, Chun-Rong; Zheng, Haiyan5; Wang, Bai-Chen
2020
Source PublicationJOURNAL OF PLANT PHYSIOLOGY
ISSN0176-1617
Volume248
AbstractLysine acetylation is one of the most important post-translational modifications and is involved in multiple cellular processes in plants. There is evidence that acetylation may play an important role in light-induced deetiolation, a key developmental switch from skotomorphogenesis to photomorphogenesis. During this transition, establishment of photosynthesis is of great significance. However, studies on acetylome dynamics during deetiolation are limited. Here, we performed the first global lysine acetylome analysis for Zea mays seedlings undergoing de-etiolation, using nano liquid chromatography coupled to tandem mass spectrometry, and identified 814 lysine-acetylated sites on 462 proteins. Bioinformatics analysis of this acetylome showed that most of the lysine-acetylated proteins are predicted to be located in the cytoplasm, nucleus, chloroplast, and mitochondria. In addition, we detected ten lysine acetylation motifs and found that the accumulation of 482 lysine-acetylated peptides corresponding to 289 proteins changed significantly during de-etiolation. These proteins include transcription factors, histones, and proteins involved in chlorophyll synthesis, photosynthesis light reaction, carbon assimilation, glycolysis, the TCA cycle, amino acid metabolism, lipid metabolism, and nucleotide metabolism. Our study provides an in-depth dataset that extends our knowledge of in vivo acetylome dynamics during de-etiolation in monocots. This dataset promotes our understanding of the functional consequences of lysine acetylation in diverse cellular metabolic regulatory processes, and will be a useful toolkit for further investigations of the lysine acetylome and de-etiolation in plants.
KeywordLysine-acetylation De-etiolation Maize seedling Photosynthesis establishment
Subject AreaPlant Sciences
DOI10.1016/j.jplph.2020.153158
Indexed BySCI
Language英语
WOS KeywordCHLOROPHYLL BIOSYNTHESIS ; PHOTOSYSTEM-II ; FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE ; PHOSPHOENOLPYRUVATE CARBOXYLASE ; TRANSCRIPTIONAL REPRESSION ; CRYSTALLOGRAPHIC ANALYSIS ; HYPOCOTYL ELONGATION ; CHROMATIN-STRUCTURE ; PHOSPHATE DIKINASE ; CRYSTAL-STRUCTURES
WOS Research AreaPlant Sciences
WOS IDWOS:000530650500004
PublisherELSEVIER GMBH
SubtypeArticle
Publication PlaceMUNICH
EISSN1618-1328
Funding OrganizationNational Key Research and Development Program of China [2016YFD0101003]
Corresponding Author Emailyanzhensky@ibcas.ac.cn ; shenzhuo@gdaas.cn ; zfgao12@ibcas.ac.cn ; chaoqing@ibcas.ac.cn ; qcr3906@163.com ; haiyanz@cabm.rutgers.edu ; wangbc@ibcas.ac.cn
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Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ibcas.ac.cn/handle/2S10CLM1/21772
Collection中科院光生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Bot, Photosynth Res Ctr, Key Lab Photobiol, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Guangdong Acad Agr Sci, Guangdong Key Lab New Technol Res Vegetables, Vegetable Res Inst, Guangzhou 510640, Peoples R China
4.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100039, Peoples R China
5.Heilongjiang Acad Agr Sci, Inst Crop Cultivat & Farming, Harbin 150086, Peoples R China
6.Rutgers State Univ, Biol Mass Spectrometry Facil, Ctr Adv Biotechnol & Med, Piscataway, NJ 08855 USA
Recommended Citation
GB/T 7714
Yan, Zhen,Shen, Zhuo,Gao, Zhi-Fang,et al. A comprehensive analysis of the lysine acetylome reveals diverse functions of acetylated proteins during de -etiolation in Zea mays[J]. JOURNAL OF PLANT PHYSIOLOGY,2020,248.
APA Yan, Zhen.,Shen, Zhuo.,Gao, Zhi-Fang.,Chao, Qing.,Qian, Chun-Rong.,...&Wang, Bai-Chen.(2020).A comprehensive analysis of the lysine acetylome reveals diverse functions of acetylated proteins during de -etiolation in Zea mays.JOURNAL OF PLANT PHYSIOLOGY,248.
MLA Yan, Zhen,et al."A comprehensive analysis of the lysine acetylome reveals diverse functions of acetylated proteins during de -etiolation in Zea mays".JOURNAL OF PLANT PHYSIOLOGY 248(2020).
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