A comprehensive analysis of the lysine acetylome reveals diverse functions of acetylated proteins during de -etiolation in Zea mays

doi:10.1016/j.jplph.2020.153158

IB-CAS > 中科院光生物学重点实验室

	A comprehensive analysis of the lysine acetylome reveals diverse functions of acetylated proteins during de -etiolation in Zea mays
	Yan, Zhen ; Shen, Zhuo 2; Gao, Zhi-Fang ; Chao, Qing ; Qian, Chun-Rong ; Zheng, Haiyan 5; Wang, Bai-Chen
	2020
发表期刊	JOURNAL OF PLANT PHYSIOLOGY
ISSN	0176-1617
卷号	248
摘要	Lysine acetylation is one of the most important post-translational modifications and is involved in multiple cellular processes in plants. There is evidence that acetylation may play an important role in light-induced deetiolation, a key developmental switch from skotomorphogenesis to photomorphogenesis. During this transition, establishment of photosynthesis is of great significance. However, studies on acetylome dynamics during deetiolation are limited. Here, we performed the first global lysine acetylome analysis for Zea mays seedlings undergoing de-etiolation, using nano liquid chromatography coupled to tandem mass spectrometry, and identified 814 lysine-acetylated sites on 462 proteins. Bioinformatics analysis of this acetylome showed that most of the lysine-acetylated proteins are predicted to be located in the cytoplasm, nucleus, chloroplast, and mitochondria. In addition, we detected ten lysine acetylation motifs and found that the accumulation of 482 lysine-acetylated peptides corresponding to 289 proteins changed significantly during de-etiolation. These proteins include transcription factors, histones, and proteins involved in chlorophyll synthesis, photosynthesis light reaction, carbon assimilation, glycolysis, the TCA cycle, amino acid metabolism, lipid metabolism, and nucleotide metabolism. Our study provides an in-depth dataset that extends our knowledge of in vivo acetylome dynamics during de-etiolation in monocots. This dataset promotes our understanding of the functional consequences of lysine acetylation in diverse cellular metabolic regulatory processes, and will be a useful toolkit for further investigations of the lysine acetylome and de-etiolation in plants.
关键词	Lysine-acetylation De-etiolation Maize seedling Photosynthesis establishment
学科领域	Plant Sciences
DOI	10.1016/j.jplph.2020.153158
收录类别	SCI
语种	英语
WOS关键词	CHLOROPHYLL BIOSYNTHESIS ; PHOTOSYSTEM-II ; FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE ; PHOSPHOENOLPYRUVATE CARBOXYLASE ; TRANSCRIPTIONAL REPRESSION ; CRYSTALLOGRAPHIC ANALYSIS ; HYPOCOTYL ELONGATION ; CHROMATIN-STRUCTURE ; PHOSPHATE DIKINASE ; CRYSTAL-STRUCTURES
WOS研究方向	Plant Sciences
WOS记录号	WOS:000530650500004
出版者	ELSEVIER GMBH
文献子类	Article
出版地	MUNICH
EISSN	1618-1328
资助机构	National Key Research and Development Program of China [2016YFD0101003]
作者邮箱	yanzhensky@ibcas.ac.cn ; shenzhuo@gdaas.cn ; zfgao12@ibcas.ac.cn ; chaoqing@ibcas.ac.cn ; qcr3906@163.com ; haiyanz@cabm.rutgers.edu ; wangbc@ibcas.ac.cn
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21772
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Photosynth Res Ctr, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Guangdong Acad Agr Sci, Guangdong Key Lab New Technol Res Vegetables, Vegetable Res Inst, Guangzhou 510640, Peoples R China 4.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100039, Peoples R China 5.Heilongjiang Acad Agr Sci, Inst Crop Cultivat & Farming, Harbin 150086, Peoples R China 6.Rutgers State Univ, Biol Mass Spectrometry Facil, Ctr Adv Biotechnol & Med, Piscataway, NJ 08855 USA
推荐引用方式 GB/T 7714	Yan, Zhen,Shen, Zhuo,Gao, Zhi-Fang,et al. A comprehensive analysis of the lysine acetylome reveals diverse functions of acetylated proteins during de -etiolation in Zea mays[J]. JOURNAL OF PLANT PHYSIOLOGY,2020,248.
APA	Yan, Zhen.,Shen, Zhuo.,Gao, Zhi-Fang.,Chao, Qing.,Qian, Chun-Rong.,...&Wang, Bai-Chen.(2020).A comprehensive analysis of the lysine acetylome reveals diverse functions of acetylated proteins during de -etiolation in Zea mays.JOURNAL OF PLANT PHYSIOLOGY,248.
MLA	Yan, Zhen,et al."A comprehensive analysis of the lysine acetylome reveals diverse functions of acetylated proteins during de -etiolation in Zea mays".JOURNAL OF PLANT PHYSIOLOGY 248(2020).

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