Role of PsbV-Tyr137 in photosystem II studied by site-directed mutagenesis in the thermophilic cyanobacterium Thermosynechococcus vulcanus

doi:10.1007/s11120-020-00753-8

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	Role of PsbV-Tyr137 in photosystem II studied by site-directed mutagenesis in the thermophilic cyanobacterium Thermosynechococcus vulcanus
	Xiao, Yanan 1; Zhu, Qingjun 1; Yang, Yanyan ; Wang, Wenda ; Kuang, Tingyun ; Shen, Jian-Ren; Han, Guangye
	2020
发表期刊	PHOTOSYNTHESIS RESEARCH
ISSN	0166-8595
卷号	146 期号:1-3 页码:41-54
摘要	PsbV (cytochrome c(550)) is one of the three extrinsic proteins of photosystem II (PSII) and functions to maintain the stability and activity of the Mn4CaO5 cluster, the catalytic center for water oxidation. PsbV-Y137 is the C-terminal residue of PsbV and is located at the exit of a hydrogen-bond network mediated by the D1-Y161-H190 residue pair. In order to examine the function of PsbV-Y137, four mutants, PsbV-Y137A, PsbV-Y137F, PsbV-Y137G, and PsbV-Y137W, were generated with Thermosynechococcus vulcanus (T. vulcanus). These mutants showed growth rates similar to that of the wild-type strain (WT); however, their oxygen-evolving activities were different. At pH 6.5, the oxygen evolution rates of Y137F and Y137W were almost identical to that of WT, whereas the oxygen evolution rates of the Y137A, Y137G mutants were 64% and 61% of WT, respectively. However, the oxygen evolution in the latter two mutants decreased less at higher pHs, suggesting that higher pHs facilitated oxygen evolution probably by facilitating proton egress in these two mutants. Furthermore, thylakoid membranes isolated from the PsbV-Y137A, PsbV-Y137G mutants exhibited much lower levels of oxygen-evolving activity than that of WT, which was found to be caused by the release of PsbV. In addition, PSII complexes purified from the PsbV-Y137A and PsbV-Y137G mutants lost all of the three extrinsic proteins but instead bind Psb27, an assembly cofactor of PSII. These results demonstrate that the PsbV-Tyr137 residue is required for the stable binding of PsbV to PSII, and the hydrogen-bond network mediated by D1-Y161-H190 is likely to function in proton egress during water oxidation.
关键词	Photosystem II Extrinsic proteins PsbV Hydrogen-bond network Site-directed mutagenesis Water-splitting
学科领域	Plant Sciences
DOI	10.1007/s11120-020-00753-8
收录类别	SCI
语种	英语
WOS关键词	EXTRINSIC PROTEINS ; CYTOCHROME C-550 ; FUNCTIONAL-PROPERTIES ; CHLOROPHYLL FLUORESCENCE ; CYANIDIUM-CALDARIUM ; MANGANESE CLUSTER ; CRYSTAL-STRUCTURE ; WATER OXIDATION ; 12-KDA PROTEIN ; RED ALGA
WOS研究方向	Plant Sciences
WOS记录号	WOS:000529115500001
出版者	SPRINGER
Special Issue	SI
文献子类	Article
出版地	DORDRECHT
EISSN	1573-5079
资助机构	National Key R&D Program of China [2017YFA0503700] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31470339] ; Strategic Priority Research Program of CAS [XDB17000000] ; Key Research Program of Frontier Sciences, CAS [QYZDY-SSW-SMC003]
作者邮箱	shen@cc.okayama-u.ac.jp ; hanguangye@ibcas.ac.cn
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21785
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Photosynth Res Ctr, Key Lab Photobiol, Inst Bot, 20 Nanxincun, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Yuquan Rd, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Innovat Acad Seed Design, 1 Beichen West Rd, Beijing 100101, Peoples R China 4.Okayama Univ, Res Inst Interdisciplinary Sci, Grad Sch Nat Sci & Technol, Tsushima Naka 3-1-1, Okayama 7008530, Japan
推荐引用方式 GB/T 7714	Xiao, Yanan,Zhu, Qingjun,Yang, Yanyan,et al. Role of PsbV-Tyr137 in photosystem II studied by site-directed mutagenesis in the thermophilic cyanobacterium Thermosynechococcus vulcanus[J]. PHOTOSYNTHESIS RESEARCH,2020,146(1-3):41-54.
APA	Xiao, Yanan.,Zhu, Qingjun.,Yang, Yanyan.,Wang, Wenda.,Kuang, Tingyun.,...&Han, Guangye.(2020).Role of PsbV-Tyr137 in photosystem II studied by site-directed mutagenesis in the thermophilic cyanobacterium Thermosynechococcus vulcanus.PHOTOSYNTHESIS RESEARCH,146(1-3),41-54.
MLA	Xiao, Yanan,et al."Role of PsbV-Tyr137 in photosystem II studied by site-directed mutagenesis in the thermophilic cyanobacterium Thermosynechococcus vulcanus".PHOTOSYNTHESIS RESEARCH 146.1-3(2020):41-54.

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