Biochemical Functions of Glutathione S-Transferase Family of Salix babylonica

doi:10.3389/fpls.2020.00364

	Biochemical Functions of Glutathione S-Transferase Family of Salix babylonica
	Zhuge, Xiang-Lin ; Xu, Hui 1; Xiu, Zhi-Jing ; Yang, Hai-Ling
	2020
发表期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
卷号	11
摘要	Glutathione S-transferases (GSTs) are ubiquitous enzymes that are encoded by a large gene family, and they contribute to the detoxification of endogenous or xenobiotic compounds and oxidative stress metabolism in plants. Although the GSTs gene family has been reported in many land plants, our knowledge of the evolution and function of the willow GSTs is still limited. In this study, 22 full-length GST genes were cloned from Salix babylonica and divided into three classes based on the conserved domain analysis, phylogenetic tree and gene structure: tau, phi and DHAR. The tissue-specific expression patterns were substantially different among the tau and phi GSTs. The Salix GST proteins showed functional divergences in the substrate specificities, substrate activities and kinetic characteristics. The site-directed mutagenesis studies revealed that a single amino acid mutation (Ile/Val53 -> Thr53) resulted in the lowest activity of SbGSTU7 among the Salix GSTs. These results suggest that non-synonymous substitution of an amino acid at the putative glutathione-binding site may play an important role in the divergence of enzymatic functions of Salix GST family.
关键词	glutathione S-transferase gene expression enzyme activity kinetic analysis salix babylonica
学科领域	Plant Sciences
DOI	10.3389/fpls.2020.00364
收录类别	SCI
语种	英语
WOS关键词	SUPERGENE FAMILY ; GENE FAMILY ; TOLERANCE ; DIVERGENCE ; EXPRESSION ; EVOLUTION ; LIGHT
WOS研究方向	Plant Sciences
WOS记录号	WOS:000528744500001
出版者	FRONTIERS MEDIA SA
文献子类	Article
出版地	LAUSANNE
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [NSFC 31570664]
作者邮箱	yhailing77@163.com
作品OA属性	Green Published, gold
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21790
专题	系统与进化植物学国家重点实验室
作者单位	1.Beijing Forestry Univ, Coll Biol Sci & Technol, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhuge, Xiang-Lin,Xu, Hui,Xiu, Zhi-Jing,et al. Biochemical Functions of Glutathione S-Transferase Family of Salix babylonica[J]. FRONTIERS IN PLANT SCIENCE,2020,11.
APA	Zhuge, Xiang-Lin,Xu, Hui,Xiu, Zhi-Jing,&Yang, Hai-Ling.(2020).Biochemical Functions of Glutathione S-Transferase Family of Salix babylonica.FRONTIERS IN PLANT SCIENCE,11.
MLA	Zhuge, Xiang-Lin,et al."Biochemical Functions of Glutathione S-Transferase Family of Salix babylonica".FRONTIERS IN PLANT SCIENCE 11(2020).

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