Identification and biochemical characterization of the glutathione reductase family from Populus trichocarpa

doi:10.1016/j.plantsci.2020.110459

	Identification and biochemical characterization of the glutathione reductase family from Populus trichocarpa
	Liu, Hai-Jing ; Wang, Xin ; Yang, Zhi-Ling ; Ren, Lin-Ling ; Qian, Ting-Ting
	2020
发表期刊	PLANT SCIENCE
ISSN	0168-9452
卷号	294
摘要	Glutathione reductase (GR; EC 1.6.4.2) is a key NADPH-dependent flavo-protein oxidoreductase which can catalyze the oxidized glutathione (GSSG) to reduced glutathione (GSH) to protect plant cells from oxidative damage induced by Reactive oxygen species (ROS) burst. To investigate the biochemical characteristics and functional divergence of Populus GR family, three GR genes (PtGR1.1/1.2/2) were cloned from Populus trichocarpa and their biochemical characteristics were analyzed in this study. All the three genes were expressed in root, stem, leaf and bud, and the expression of PtGR genes were general upregulated under salicylic acid and alamethicin treatment. PtGR1.1 and PtGR1.2 were localized in cytoplasm, while PtGR2 was in chloroplast. The three PtGR proteins showed different enzymatic activities, apparent kinetic characteristic and thermal stability profiles. However, they have similar bivalent metal ions (Cu2+, Cd2+, Zn2+ and Pb2+) sensitivity and optimum pH profiles. Our study sheds light on a comprehensive information of glutathione reductase family in P. trichocarpa, and proved PtGR genes play critical roles when suffering different stresses.
关键词	Glutathione reductase Subcellular localization Expression pattern Biochemical characteristics Populus trichocarpa
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
DOI	10.1016/j.plantsci.2020.110459
收录类别	SCI
语种	英语
WOS关键词	INDUCED OXIDATIVE STRESS ; MOLECULAR-CLONING ; GENE-EXPRESSION ; ENZYME-ACTIVITY ; PLANT ; OXYGEN ; DETOXIFICATION ; EVOLUTION ; ASCORBATE ; GENOME
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
WOS记录号	WOS:000528164300014
出版者	ELSEVIER IRELAND LTD
文献子类	Article
出版地	CLARE
作者邮箱	liuhj@imu.edu.cn ; qiantingting.08@163.com
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21794
专题	系统与进化植物学国家重点实验室
作者单位	1.Inner Mongolia Univ, Sch Life Sci, Key Lab Herbage & Endem Crop Biotechnol, Hohhot 010070, Peoples R China 2.[Liu, Hai-Jing 3.Wang, Xin 4.Yang, Zhi-Ling 5.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 7.Yunnan Agr Univ, Coll Agron & Biotechnol, Kunming 650201, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Liu, Hai-Jing,Wang, Xin,Yang, Zhi-Ling,et al. Identification and biochemical characterization of the glutathione reductase family from Populus trichocarpa[J]. PLANT SCIENCE,2020,294.
APA	Liu, Hai-Jing,Wang, Xin,Yang, Zhi-Ling,Ren, Lin-Ling,&Qian, Ting-Ting.(2020).Identification and biochemical characterization of the glutathione reductase family from Populus trichocarpa.PLANT SCIENCE,294.
MLA	Liu, Hai-Jing,et al."Identification and biochemical characterization of the glutathione reductase family from Populus trichocarpa".PLANT SCIENCE 294(2020).

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