IB-CAS  > 中科院北方资源植物重点实验室
A key 'foxy' aroma gene is regulated by homology-induced promoter indels in the iconic juice grape 'Concord'
Yang, Yingzhen; Cuenca, Jose8; Wang, Nian9; Liang, Zhenchang1; Sun, Honghe2; Gutierrez, Benjamin3; Xi, Xiaojun3,4; Arro, Jie3; Wang, Yi1,5; Fan, Peige1; Londo, Jason; Cousins, Peter6; Li, Shaohua1; Fei, Zhangjun2,7; Zhong, Gan-Yuan
2020
Source PublicationHORTICULTURE RESEARCH
ISSN2662-6810
Volume7Issue:1
Abstract'Concord', the most well-known juice grape with a parentage of the North American grape species Vitis labrusca L., possesses a special 'foxy' aroma predominantly resulted from the accumulation of methyl anthranilate (MA) in berries. This aroma, however, is often perceived as an undesirable attribute by wine consumers and rarely noticeable in the common table and wine grape species V. vinifera. Here we discovered homology-induced promoter indels as a major genetic mechanism for species-specific regulation of a key 'foxy' aroma gene, anthraniloyl-CoA:methanol acyltransferase (AMAT), that is responsible for MA biosynthesis. We found the absence of a 426-bp and/or a 42-bp sequence in AMAT promoters highly associated with high levels of AMAT expression and MA accumulation in 'Concord' and other V. labrusca-derived grapes. These promoter variants, all with direct and inverted repeats, were further confirmed in more than 1,300 Vitis germplasm. Moreover, functional impact of these indels was validated in transgenic Arabidopsis. Superimposed on the promoter regulation, large structural changes including exonic insertion of a retrotransposon were present at the AMAT locus in some V. vinifera grapes. Elucidation of the AMAT genetic regulation advances our understanding of the 'foxy' aroma trait and makes it genetically trackable and amenable in grapevine breeding.
Subject AreaPlant Sciences ; Genetics & Heredity ; Horticulture
DOI10.1038/s41438-020-0304-6
Indexed BySCI
Language英语
WOS KeywordMETHYL ANTHRANILATE ; VITIS-VINIFERA ; REPLICATION ; INSTABILITY ; ESTERS ; SKIN ; WAX
WOS Research AreaPlant Sciences ; Genetics & Heredity ; Agriculture
WOS IDWOS:000526545600001
PublisherNANJING AGRICULTURAL UNIV
SubtypeArticle
Publication PlaceNANJING
EISSN2052-7276
Funding OrganizationUSDA Agricultural Research ServiceUnited States Department of Agriculture (USDA)USDA Agricultural Research Service
Corresponding Author Emailganyuan.zhong@ars.usda.gov
OAgold, Green Published
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ibcas.ac.cn/handle/2S10CLM1/21804
Collection中科院北方资源植物重点实验室
Affiliation1.USDA ARS, Grape Genet Res Unit, Geneva, NY 14456 USA
2.Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Enol, Lab Plant Resources, Beijing, Peoples R China
3.Cornell Univ, Boyce Thompson Inst Plant Res, Tower Rd, Ithaca, NY 14853 USA
4.USDA ARS, Plant Genet Resources Unit, Geneva, NY USA
5.Shanghai Acad Agr Sci, Forestry & Pomol Res Inst, Shanghai, Peoples R China
6.Univ Chinese Acad Sci, Beijing, Peoples R China
7.E&J Gallo Winery, Modesto, CA USA
8.USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA
9.Inst Valenciano Invest Agr, Ctr Citricultura & Prod Vegetal, Valencia, Spain
10.Huazhong Agr Univ, Coll Hort & Forestry, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Yang, Yingzhen,Cuenca, Jose,Wang, Nian,et al. A key 'foxy' aroma gene is regulated by homology-induced promoter indels in the iconic juice grape 'Concord'[J]. HORTICULTURE RESEARCH,2020,7(1).
APA Yang, Yingzhen.,Cuenca, Jose.,Wang, Nian.,Liang, Zhenchang.,Sun, Honghe.,...&Zhong, Gan-Yuan.(2020).A key 'foxy' aroma gene is regulated by homology-induced promoter indels in the iconic juice grape 'Concord'.HORTICULTURE RESEARCH,7(1).
MLA Yang, Yingzhen,et al."A key 'foxy' aroma gene is regulated by homology-induced promoter indels in the iconic juice grape 'Concord'".HORTICULTURE RESEARCH 7.1(2020).
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