IB-CAS  > 中科院北方资源植物重点实验室
A key 'foxy' aroma gene is regulated by homology-induced promoter indels in the iconic juice grape 'Concord'
Yang, Yingzhen; Cuenca, Jose8; Wang, Nian9; Liang, Zhenchang1; Sun, Honghe2; Gutierrez, Benjamin3; Xi, Xiaojun3,4; Arro, Jie3; Wang, Yi1,5; Fan, Peige1; Londo, Jason; Cousins, Peter6; Li, Shaohua1; Fei, Zhangjun2,7; Zhong, Gan-Yuan
Abstract'Concord', the most well-known juice grape with a parentage of the North American grape species Vitis labrusca L., possesses a special 'foxy' aroma predominantly resulted from the accumulation of methyl anthranilate (MA) in berries. This aroma, however, is often perceived as an undesirable attribute by wine consumers and rarely noticeable in the common table and wine grape species V. vinifera. Here we discovered homology-induced promoter indels as a major genetic mechanism for species-specific regulation of a key 'foxy' aroma gene, anthraniloyl-CoA:methanol acyltransferase (AMAT), that is responsible for MA biosynthesis. We found the absence of a 426-bp and/or a 42-bp sequence in AMAT promoters highly associated with high levels of AMAT expression and MA accumulation in 'Concord' and other V. labrusca-derived grapes. These promoter variants, all with direct and inverted repeats, were further confirmed in more than 1,300 Vitis germplasm. Moreover, functional impact of these indels was validated in transgenic Arabidopsis. Superimposed on the promoter regulation, large structural changes including exonic insertion of a retrotransposon were present at the AMAT locus in some V. vinifera grapes. Elucidation of the AMAT genetic regulation advances our understanding of the 'foxy' aroma trait and makes it genetically trackable and amenable in grapevine breeding.
Subject AreaPlant Sciences ; Genetics & Heredity ; Horticulture
Indexed BySCI
WOS Research AreaPlant Sciences ; Genetics & Heredity ; Agriculture
WOS IDWOS:000526545600001
Publication PlaceNANJING
Funding OrganizationUSDA Agricultural Research ServiceUnited States Department of Agriculture (USDA)USDA Agricultural Research Service
Corresponding Author Emailganyuan.zhong@ars.usda.gov
OAgold, Green Published
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Affiliation1.USDA ARS, Grape Genet Res Unit, Geneva, NY 14456 USA
2.Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Enol, Lab Plant Resources, Beijing, Peoples R China
3.Cornell Univ, Boyce Thompson Inst Plant Res, Tower Rd, Ithaca, NY 14853 USA
4.USDA ARS, Plant Genet Resources Unit, Geneva, NY USA
5.Shanghai Acad Agr Sci, Forestry & Pomol Res Inst, Shanghai, Peoples R China
6.Univ Chinese Acad Sci, Beijing, Peoples R China
7.E&J Gallo Winery, Modesto, CA USA
8.USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA
9.Inst Valenciano Invest Agr, Ctr Citricultura & Prod Vegetal, Valencia, Spain
10.Huazhong Agr Univ, Coll Hort & Forestry, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Yang, Yingzhen,Cuenca, Jose,Wang, Nian,et al. A key 'foxy' aroma gene is regulated by homology-induced promoter indels in the iconic juice grape 'Concord'[J]. HORTICULTURE RESEARCH,2020,7(1).
APA Yang, Yingzhen.,Cuenca, Jose.,Wang, Nian.,Liang, Zhenchang.,Sun, Honghe.,...&Zhong, Gan-Yuan.(2020).A key 'foxy' aroma gene is regulated by homology-induced promoter indels in the iconic juice grape 'Concord'.HORTICULTURE RESEARCH,7(1).
MLA Yang, Yingzhen,et al."A key 'foxy' aroma gene is regulated by homology-induced promoter indels in the iconic juice grape 'Concord'".HORTICULTURE RESEARCH 7.1(2020).
Files in This Item:
File Name/Size DocType Version Access License
Yang-2020-A key 'fox(1115KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yang, Yingzhen]'s Articles
[Cuenca, Jose]'s Articles
[Wang, Nian]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yang, Yingzhen]'s Articles
[Cuenca, Jose]'s Articles
[Wang, Nian]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yang, Yingzhen]'s Articles
[Cuenca, Jose]'s Articles
[Wang, Nian]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Yang-2020-A key 'foxy' aroma gene is regulated.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.