A key 'foxy' aroma gene is regulated by homology-induced promoter indels in the iconic juice grape 'Concord'

doi:10.1038/s41438-020-0304-6

	A key 'foxy' aroma gene is regulated by homology-induced promoter indels in the iconic juice grape 'Concord'
	Yang, Yingzhen ; Cuenca, Jose 8; Wang, Nian 9; Liang, Zhenchang 1; Sun, Honghe 2; Gutierrez, Benjamin 3; Xi, Xiaojun 3,4; Arro, Jie 3; Wang, Yi 1,5; Fan, Peige 1; Londo, Jason ; Cousins, Peter 6; Li, Shaohua1 ; Fei, Zhangjun 2,7; Zhong, Gan-Yuan
	2020
发表期刊	HORTICULTURE RESEARCH
ISSN	2662-6810
卷号	7 期号:1
摘要	'Concord', the most well-known juice grape with a parentage of the North American grape species Vitis labrusca L., possesses a special 'foxy' aroma predominantly resulted from the accumulation of methyl anthranilate (MA) in berries. This aroma, however, is often perceived as an undesirable attribute by wine consumers and rarely noticeable in the common table and wine grape species V. vinifera. Here we discovered homology-induced promoter indels as a major genetic mechanism for species-specific regulation of a key 'foxy' aroma gene, anthraniloyl-CoA:methanol acyltransferase (AMAT), that is responsible for MA biosynthesis. We found the absence of a 426-bp and/or a 42-bp sequence in AMAT promoters highly associated with high levels of AMAT expression and MA accumulation in 'Concord' and other V. labrusca-derived grapes. These promoter variants, all with direct and inverted repeats, were further confirmed in more than 1,300 Vitis germplasm. Moreover, functional impact of these indels was validated in transgenic Arabidopsis. Superimposed on the promoter regulation, large structural changes including exonic insertion of a retrotransposon were present at the AMAT locus in some V. vinifera grapes. Elucidation of the AMAT genetic regulation advances our understanding of the 'foxy' aroma trait and makes it genetically trackable and amenable in grapevine breeding.
学科领域	Plant Sciences ; Genetics & Heredity ; Horticulture
DOI	10.1038/s41438-020-0304-6
收录类别	SCI
语种	英语
WOS关键词	METHYL ANTHRANILATE ; VITIS-VINIFERA ; REPLICATION ; INSTABILITY ; ESTERS ; SKIN ; WAX
WOS研究方向	Plant Sciences ; Genetics & Heredity ; Agriculture
WOS记录号	WOS:000526545600001
出版者	NANJING AGRICULTURAL UNIV
文献子类	Article
出版地	NANJING
EISSN	2052-7276
资助机构	USDA Agricultural Research ServiceUnited States Department of Agriculture (USDA)USDA Agricultural Research Service
作者邮箱	ganyuan.zhong@ars.usda.gov
作品OA属性	gold, Green Published
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21804
专题	中科院北方资源植物重点实验室
作者单位	1.USDA ARS, Grape Genet Res Unit, Geneva, NY 14456 USA 2.Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Enol, Lab Plant Resources, Beijing, Peoples R China 3.Cornell Univ, Boyce Thompson Inst Plant Res, Tower Rd, Ithaca, NY 14853 USA 4.USDA ARS, Plant Genet Resources Unit, Geneva, NY USA 5.Shanghai Acad Agr Sci, Forestry & Pomol Res Inst, Shanghai, Peoples R China 6.Univ Chinese Acad Sci, Beijing, Peoples R China 7.E&J Gallo Winery, Modesto, CA USA 8.USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA 9.Inst Valenciano Invest Agr, Ctr Citricultura & Prod Vegetal, Valencia, Spain 10.Huazhong Agr Univ, Coll Hort & Forestry, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Yang, Yingzhen,Cuenca, Jose,Wang, Nian,et al. A key 'foxy' aroma gene is regulated by homology-induced promoter indels in the iconic juice grape 'Concord'[J]. HORTICULTURE RESEARCH,2020,7(1).
APA	Yang, Yingzhen.,Cuenca, Jose.,Wang, Nian.,Liang, Zhenchang.,Sun, Honghe.,...&Zhong, Gan-Yuan.(2020).A key 'foxy' aroma gene is regulated by homology-induced promoter indels in the iconic juice grape 'Concord'.HORTICULTURE RESEARCH,7(1).
MLA	Yang, Yingzhen,et al."A key 'foxy' aroma gene is regulated by homology-induced promoter indels in the iconic juice grape 'Concord'".HORTICULTURE RESEARCH 7.1(2020).

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Yang-2020-A key 'fox（1115KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文