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The critical function of the plastid rRNA methyltransferase, CMAL, in ribosome biogenesis and plant development | |
Zou, Meijuan; Mu, Ying; Chai, Xin1; Ouyang, Min; Yu, Long-Jiang; Zhang, Lixin2; Meurer, Joerg3; Chi, Wei1 | |
2020 | |
发表期刊 | NUCLEIC ACIDS RESEARCH |
ISSN | 0305-1048 |
卷号 | 48期号:6页码:3195-3210 |
摘要 | Methylation of nucleotides in ribosomal RNAs (rRNAs) is a ubiquitous feature that occurs in all living organisms. The formation of methylated nucleotides is performed by a variety of RNA-methyltransferases. Chloroplasts of plant cells result from an endosymbiotic event and possess their own genome and ribosomes. However, enzymes responsible for rRNA methylation and the function of modified nucleotides in chloroplasts remain to be determined. Here, we identified an rRNA methyltransferase, CMAL (Chloroplast MraW-Like), in the Arabidopsis chloroplast and investigated its function. CMAL is the Arabidopsis ortholog of bacterial MraW/RsmH proteins and accounts to the N-4-methylation of C-1352 in chloroplast 16S rRNA, indicating that CMAL orthologs and this methyl-modification nucleotide is conserved between bacteria and the endosymbiont-derived eukaryotic organelle. The knockout of CMAL in Arabidopsis impairs the chloroplast ribosome accumulation and accordingly reduced the efficiency of mRNA translation. Interestingly, the loss of CMAL leads not only to defects in chloroplast function, but also to abnormal leaf and root development and overall plant morphology. Further investigation showed that CMAL is involved in the plant development probably by modulating auxin derived signaling pathways. This study uncovered the important role of 16S rRNA methylation mediated by CMAL in chloroplast ribosome biogenesis and plant development. |
学科领域 | Biochemistry & Molecular Biology |
DOI | 10.1093/nar/gkaa129 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | CHLOROPLAST RIBOSOME ; ESCHERICHIA-COLI ; AUXIN BIOSYNTHESIS ; MITOCHONDRIAL BIOGENESIS ; ARABIDOPSIS-THALIANA ; GENE-EXPRESSION ; MESSENGER-RNA ; TRANSLATION ; PROTEIN ; IDENTIFICATION |
WOS研究方向 | Biochemistry & Molecular Biology |
WOS记录号 | WOS:000525957500034 |
出版者 | OXFORD UNIV PRESS |
文献子类 | Article |
出版地 | OXFORD |
EISSN | 1362-4962 |
资助机构 | Strategic Priority Research Program of CAS [XDB17030100] ; Ministry of Agriculture of ChinaMinistry of Agriculture, China [2016ZX08009003-005] ; German Science FoundationGerman Research Foundation (DFG) [TRR 175] |
作者邮箱 | chiweimr@ibcas.ac.cn |
作品OA属性 | Green Published, gold |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/21810 |
专题 | 中科院光生物学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Henan Univ, Sch Life Sci, Key Lab Plant Stress Biol, State Key Lab Cotton Biol, Kaifeng 475004, Peoples R China 4.Ludwig Maximilians Univ Munchen, Fac Biol, Plant Mol Biol, D-82152 Planegg Martinsried, Germany |
推荐引用方式 GB/T 7714 | Zou, Meijuan,Mu, Ying,Chai, Xin,et al. The critical function of the plastid rRNA methyltransferase, CMAL, in ribosome biogenesis and plant development[J]. NUCLEIC ACIDS RESEARCH,2020,48(6):3195-3210. |
APA | Zou, Meijuan.,Mu, Ying.,Chai, Xin.,Ouyang, Min.,Yu, Long-Jiang.,...&Chi, Wei.(2020).The critical function of the plastid rRNA methyltransferase, CMAL, in ribosome biogenesis and plant development.NUCLEIC ACIDS RESEARCH,48(6),3195-3210. |
MLA | Zou, Meijuan,et al."The critical function of the plastid rRNA methyltransferase, CMAL, in ribosome biogenesis and plant development".NUCLEIC ACIDS RESEARCH 48.6(2020):3195-3210. |
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