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The critical function of the plastid rRNA methyltransferase, CMAL, in ribosome biogenesis and plant development
Zou, Meijuan; Mu, Ying; Chai, Xin1; Ouyang, Min; Yu, Long-Jiang; Zhang, Lixin2; Meurer, Joerg3; Chi, Wei1
2020
Source PublicationNUCLEIC ACIDS RESEARCH
ISSN0305-1048
Volume48Issue:6Pages:3195-3210
AbstractMethylation of nucleotides in ribosomal RNAs (rRNAs) is a ubiquitous feature that occurs in all living organisms. The formation of methylated nucleotides is performed by a variety of RNA-methyltransferases. Chloroplasts of plant cells result from an endosymbiotic event and possess their own genome and ribosomes. However, enzymes responsible for rRNA methylation and the function of modified nucleotides in chloroplasts remain to be determined. Here, we identified an rRNA methyltransferase, CMAL (Chloroplast MraW-Like), in the Arabidopsis chloroplast and investigated its function. CMAL is the Arabidopsis ortholog of bacterial MraW/RsmH proteins and accounts to the N-4-methylation of C-1352 in chloroplast 16S rRNA, indicating that CMAL orthologs and this methyl-modification nucleotide is conserved between bacteria and the endosymbiont-derived eukaryotic organelle. The knockout of CMAL in Arabidopsis impairs the chloroplast ribosome accumulation and accordingly reduced the efficiency of mRNA translation. Interestingly, the loss of CMAL leads not only to defects in chloroplast function, but also to abnormal leaf and root development and overall plant morphology. Further investigation showed that CMAL is involved in the plant development probably by modulating auxin derived signaling pathways. This study uncovered the important role of 16S rRNA methylation mediated by CMAL in chloroplast ribosome biogenesis and plant development.
Subject AreaBiochemistry & Molecular Biology
DOI10.1093/nar/gkaa129
Indexed BySCI
Language英语
WOS KeywordCHLOROPLAST RIBOSOME ; ESCHERICHIA-COLI ; AUXIN BIOSYNTHESIS ; MITOCHONDRIAL BIOGENESIS ; ARABIDOPSIS-THALIANA ; GENE-EXPRESSION ; MESSENGER-RNA ; TRANSLATION ; PROTEIN ; IDENTIFICATION
WOS Research AreaBiochemistry & Molecular Biology
WOS IDWOS:000525957500034
PublisherOXFORD UNIV PRESS
SubtypeArticle
Publication PlaceOXFORD
EISSN1362-4962
Funding OrganizationStrategic Priority Research Program of CAS [XDB17030100] ; Ministry of Agriculture of ChinaMinistry of Agriculture, China [2016ZX08009003-005] ; German Science FoundationGerman Research Foundation (DFG) [TRR 175]
Corresponding Author Emailchiweimr@ibcas.ac.cn
OAGreen Published, gold
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ibcas.ac.cn/handle/2S10CLM1/21810
Collection中科院光生物学重点实验室
Affiliation1.Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Henan Univ, Sch Life Sci, Key Lab Plant Stress Biol, State Key Lab Cotton Biol, Kaifeng 475004, Peoples R China
4.Ludwig Maximilians Univ Munchen, Fac Biol, Plant Mol Biol, D-82152 Planegg Martinsried, Germany
Recommended Citation
GB/T 7714
Zou, Meijuan,Mu, Ying,Chai, Xin,et al. The critical function of the plastid rRNA methyltransferase, CMAL, in ribosome biogenesis and plant development[J]. NUCLEIC ACIDS RESEARCH,2020,48(6):3195-3210.
APA Zou, Meijuan.,Mu, Ying.,Chai, Xin.,Ouyang, Min.,Yu, Long-Jiang.,...&Chi, Wei.(2020).The critical function of the plastid rRNA methyltransferase, CMAL, in ribosome biogenesis and plant development.NUCLEIC ACIDS RESEARCH,48(6),3195-3210.
MLA Zou, Meijuan,et al."The critical function of the plastid rRNA methyltransferase, CMAL, in ribosome biogenesis and plant development".NUCLEIC ACIDS RESEARCH 48.6(2020):3195-3210.
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