Excitation dynamics and relaxation in the major antenna of a marine green alga Bryopsis corticulans

doi:10.1016/j.bbabio.2020.148186

IB-CAS > 中科院光生物学重点实验室

	Excitation dynamics and relaxation in the major antenna of a marine green alga Bryopsis corticulans
	Li, Dan-Hong ; Wang, Wenda ; Zhou, Cuicui ; Zhang, Yan ; Wang, Peng ; Shen, Jian-Ren; Kuang, Tingyun 7; Zhang, Jian-Ping
	2020
发表期刊	BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
ISSN	0005-2728
卷号	1861 期号:5-6
摘要	The light-harvesting complexes II (LHCIIs) of spinach and Bryopsis corticulans as a green alga are similar in structure, but differ in carotenoid (Car) and chlorophyll (Chl) compositions. Carbonyl Cars siphonein (Spn) and siphonaxanthin (Spx) bind to B. corticulans LHCII likely in the sites as a pair of lutein (Lut) molecules bind to spinach LHCII in the central domain. To understand the light-harvesting and photoprotective properties of the algal LHCII, we compared its excitation dynamics and relaxation to those of spinach LHCII been well documented. It was found that B. corticulans LHCII exhibited a substantially longer chlorophyll (Chl) fluorescence lifetime (4.9 ns vs 4.1 ns) and a 60% increase of the fluorescence quantum yield. Photoexcitation populated (3)Car* equally between Spn and Spx in B. corticulans LHCII, whereas predominantly at Lut620 in spinach LHCII. These results prove the functional differences of the LHCIIs with different Car pairs and Chl a/b ratios: B. corticulans LHCII shows the enhanced blue-green light absorption, the alleviated quenching of 1 Chl, and the dual sites of quenching (3)Chl, which may facilitate its light-harvesting and photoprotection functions. Moreover, for both types of LHCIIs, the triplet excitation profiles revealed the involvement of extra (3)Car* formation mechanisms besides the conventional Chl-to-Car triplet transfer, which are discussed in relation to the ultrafast processes of (1)Chl* quenching. Our experimental findings will be helpful in deepening the understanding of the light harvesting and photoprotection functions of B. corticulans living in the intertidal zone with dramatically changing light condition.
关键词	Carotenoids Marine algae Light harvesting Photoprotection Photosynthesis
学科领域	Biochemistry & Molecular Biology ; Biophysics
DOI	10.1016/j.bbabio.2020.148186
收录类别	SCI
语种	英语
WOS关键词	LIGHT-HARVESTING-COMPLEX ; ENERGY-TRANSFER ; PHOTOSYSTEM-II ; PERIDININ-CHLOROPHYLL ; TRIPLET FORMATION ; CATION FORMATION ; STRUCTURAL BASIS ; SINGLET FISSION ; EXCITED-STATES ; HIGHER-PLANTS
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS记录号	WOS:000523619700004
出版者	ELSEVIER
文献子类	Article
出版地	AMSTERDAM
EISSN	1879-2650
资助机构	Natural Science Foundation of China (NSFC)National Natural Science Foundation of China (NSFC) [21673288, 31600191] ; National Key Research and Development Program of China [2017YFA0503700] ; CAS Key Research program for Frontier Science [QYZDY-SSW-SMC003]
作者邮箱	lidh01@ruc.edu.cn ; wdwang@ibcas.ac.cn ; zhoucc@ibcas.ac.cn ; yerzhang@ruc.edu.cn ; wpeng_chem@ruc.edu.cn ; shen@cc.okayama-u.ac.jp ; kuangty@ibcas.ac.cn ; jpzhang@ruc.edu.cn
作品OA属性	Bronze
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21829
专题	中科院光生物学重点实验室
作者单位	1.[Li, Dan-Hong 2.Zhang, Yan 3.Wang, Peng 4.Renmin Univ China, Dept Chem, Beijing 100872, Peoples R China 5.[Wang, Wenda 6.Zhou, Cuicui 7.Shen, Jian-Ren 8.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Li, Dan-Hong,Wang, Wenda,Zhou, Cuicui,et al. Excitation dynamics and relaxation in the major antenna of a marine green alga Bryopsis corticulans[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,2020,1861(5-6).
APA	Li, Dan-Hong.,Wang, Wenda.,Zhou, Cuicui.,Zhang, Yan.,Wang, Peng.,...&Zhang, Jian-Ping.(2020).Excitation dynamics and relaxation in the major antenna of a marine green alga Bryopsis corticulans.BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,1861(5-6).
MLA	Li, Dan-Hong,et al."Excitation dynamics and relaxation in the major antenna of a marine green alga Bryopsis corticulans".BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS 1861.5-6(2020).

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