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| Excitation dynamics and relaxation in the major antenna of a marine green alga Bryopsis corticulans | |
Li, Dan-Hong; Wang, Wenda; Zhou, Cuicui; Zhang, Yan; Wang, Peng; Shen, Jian-Ren ; Kuang, Tingyun7; Zhang, Jian-Ping
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| 2020 | |
| Source Publication | BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
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| ISSN | 0005-2728 |
| Volume | 1861Issue:5-6 |
| Abstract | The light-harvesting complexes II (LHCIIs) of spinach and Bryopsis corticulans as a green alga are similar in structure, but differ in carotenoid (Car) and chlorophyll (Chl) compositions. Carbonyl Cars siphonein (Spn) and siphonaxanthin (Spx) bind to B. corticulans LHCII likely in the sites as a pair of lutein (Lut) molecules bind to spinach LHCII in the central domain. To understand the light-harvesting and photoprotective properties of the algal LHCII, we compared its excitation dynamics and relaxation to those of spinach LHCII been well documented. It was found that B. corticulans LHCII exhibited a substantially longer chlorophyll (Chl) fluorescence lifetime (4.9 ns vs 4.1 ns) and a 60% increase of the fluorescence quantum yield. Photoexcitation populated (3)Car* equally between Spn and Spx in B. corticulans LHCII, whereas predominantly at Lut620 in spinach LHCII. These results prove the functional differences of the LHCIIs with different Car pairs and Chl a/b ratios: B. corticulans LHCII shows the enhanced blue-green light absorption, the alleviated quenching of 1 Chl*, and the dual sites of quenching (3)Chl*, which may facilitate its light-harvesting and photoprotection functions. Moreover, for both types of LHCIIs, the triplet excitation profiles revealed the involvement of extra (3)Car* formation mechanisms besides the conventional Chl-to-Car triplet transfer, which are discussed in relation to the ultrafast processes of (1)Chl* quenching. Our experimental findings will be helpful in deepening the understanding of the light harvesting and photoprotection functions of B. corticulans living in the intertidal zone with dramatically changing light condition. |
| Keyword | Carotenoids Marine algae Light harvesting Photoprotection Photosynthesis |
| Subject Area | Biochemistry & Molecular Biology ; Biophysics |
| DOI | 10.1016/j.bbabio.2020.148186 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | LIGHT-HARVESTING-COMPLEX ; ENERGY-TRANSFER ; PHOTOSYSTEM-II ; PERIDININ-CHLOROPHYLL ; TRIPLET FORMATION ; CATION FORMATION ; STRUCTURAL BASIS ; SINGLET FISSION ; EXCITED-STATES ; HIGHER-PLANTS |
| WOS Research Area | Biochemistry & Molecular Biology ; Biophysics |
| WOS ID | WOS:000523619700004 |
| Publisher | ELSEVIER |
| Subtype | Article |
| Publication Place | AMSTERDAM |
| EISSN | 1879-2650 |
| Funding Organization | Natural Science Foundation of China (NSFC)National Natural Science Foundation of China (NSFC) [21673288, 31600191] ; National Key Research and Development Program of China [2017YFA0503700] ; CAS Key Research program for Frontier Science [QYZDY-SSW-SMC003] |
| Corresponding Author Email | lidh01@ruc.edu.cn ; wdwang@ibcas.ac.cn ; zhoucc@ibcas.ac.cn ; yerzhang@ruc.edu.cn ; wpeng_chem@ruc.edu.cn ; shen@cc.okayama-u.ac.jp ; kuangty@ibcas.ac.cn ; jpzhang@ruc.edu.cn |
| OA | Bronze |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/21829 |
| Collection | 中科院光生物学重点实验室 |
| Affiliation | 1.[Li, Dan-Hong 2.Zhang, Yan 3.Wang, Peng 4.Renmin Univ China, Dept Chem, Beijing 100872, Peoples R China 5.[Wang, Wenda 6.Zhou, Cuicui 7.Shen, Jian-Ren 8.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China |
| Recommended Citation GB/T 7714 | Li, Dan-Hong,Wang, Wenda,Zhou, Cuicui,et al. Excitation dynamics and relaxation in the major antenna of a marine green alga Bryopsis corticulans[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,2020,1861(5-6). |
| APA | Li, Dan-Hong.,Wang, Wenda.,Zhou, Cuicui.,Zhang, Yan.,Wang, Peng.,...&Zhang, Jian-Ping.(2020).Excitation dynamics and relaxation in the major antenna of a marine green alga Bryopsis corticulans.BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,1861(5-6). |
| MLA | Li, Dan-Hong,et al."Excitation dynamics and relaxation in the major antenna of a marine green alga Bryopsis corticulans".BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS 1861.5-6(2020). |
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| 1-s2.0-S000527282030（2064KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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