IB-CAS  > 中科院光生物学重点实验室
Functional analysis of ZmCOP1 and ZmHY5 reveals conserved light signaling mechanism in maize and Arabidopsis
Huai, Junling; Jing, Yanjun; Lin, Rongcheng1,2
2020
发表期刊PHYSIOLOGIA PLANTARUM
ISSN0031-9317
卷号169期号:3页码:369-379
摘要Plants have evolved light signaling mechanisms to optimally adapt developmental patterns to the ambient light environments. CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1) and LONG HYPOCOTYL5 (HY5) are two critical components in the light signaling pathway in Arabidopsis thaliana. COP1 acts as an E3 ubiquitin ligase that targets positive regulators, such as HY5, leading to their degradation in darkness. However, functional analysis of the COP1-HY5 module in maize (Zea mays) has not been reported. Here, we investigated the expression patterns and roles of the COP1 and HY5 orthologs, ZmCOP1 and ZmHY5, in regulating photomorphogenesis. These two genes have high amino acid identities with their Arabidopsis homolog and were both regulated by light. Subcellular localization assay showed that ZmCOP1 was distributed in the cytosol and ZmHY5 localized in the nucleus. Exogenous expression of ZmCOP1 rescued the physiological defects of the cop1-4 mutant, and expression of ZmHY5 complemented the long hypocotyl phenotype of the hy5-215 mutant in Arabidopsis. Yeast two-hybrid and fluorescence resonance energy transfer assays showed that ZmCOP1 interacted with ZmHY5. Our study gains insight into the conserved function and regulatory mechanism of the COP1-HY5 signaling pathway in maize and Arabidopsis.
学科领域Plant Sciences
DOI10.1111/ppl.13099
收录类别SCI
语种英语
WOS关键词TRANSCRIPTION FACTOR HY5 ; GENE-EXPRESSION ; COP1 GENE ; FINGER MOTIF ; BZIP PROTEIN ; PHYTOCHROME ; BIOSYNTHESIS ; ROLES ; TRANSDUCTION ; INTERACTS
WOS研究方向Plant Sciences
WOS记录号WOS:000523269500001
出版者WILEY
Special IssueSI
文献子类Article
出版地HOBOKEN
EISSN1399-3054
资助机构National Key Research and Development Program of China [2016YFD0100405] ; Ministry of Agriculture of ChinaMinistry of Agriculture, China [2016ZX08009-003] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31800235]
作者邮箱rclin@ibcas.ac.cn
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/21831
专题中科院光生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, Beijing 100093, Peoples R China
推荐引用方式
GB/T 7714
Huai, Junling,Jing, Yanjun,Lin, Rongcheng. Functional analysis of ZmCOP1 and ZmHY5 reveals conserved light signaling mechanism in maize and Arabidopsis[J]. PHYSIOLOGIA PLANTARUM,2020,169(3):369-379.
APA Huai, Junling,Jing, Yanjun,&Lin, Rongcheng.(2020).Functional analysis of ZmCOP1 and ZmHY5 reveals conserved light signaling mechanism in maize and Arabidopsis.PHYSIOLOGIA PLANTARUM,169(3),369-379.
MLA Huai, Junling,et al."Functional analysis of ZmCOP1 and ZmHY5 reveals conserved light signaling mechanism in maize and Arabidopsis".PHYSIOLOGIA PLANTARUM 169.3(2020):369-379.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Huai-2020-Functional(15391KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Huai, Junling]的文章
[Jing, Yanjun]的文章
[Lin, Rongcheng]的文章
百度学术
百度学术中相似的文章
[Huai, Junling]的文章
[Jing, Yanjun]的文章
[Lin, Rongcheng]的文章
必应学术
必应学术中相似的文章
[Huai, Junling]的文章
[Jing, Yanjun]的文章
[Lin, Rongcheng]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Huai-2020-Functional analysis of ZmCOP1 and Zm.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。