Vacuolar invertase genes SbVIN1 and SbVIN2 are differently associated with stem and grain traits in sorghum (Sorghum bicolor)

doi:10.1016/j.cj.2019.06.012

	Vacuolar invertase genes SbVIN1 and SbVIN2 are differently associated with stem and grain traits in sorghum (Sorghum bicolor)
	Chi, Yunhua 1; Wilson, Kimani 1; Liu, Zhiquan 2; Wu, Xiaoyuan ; Shang, Li ; Zhang, Limin ; Jing, Haichun1,2 ; Hao, Huaiqing
	2020
发表期刊	CROP JOURNAL
ISSN	2095-5421
卷号	8 期号:2 页码:299-312
摘要	In higher plants, vacuolar invertases play essential roles in sugar metabolism, organ development, and sink strength. In sorghum (Sorghum bicolor), two vacuolar invertase genes, SbVIN1 (Sobic. 004G004800) and SbVIN2 (Sobic. 006G160700) have been reported, but their enzymatic properties and functional differences are largely unknown. We combined molecular, biochemical and genomic approaches to investigate their roles in sorghum stem and grain traits. SbVIN1 and SbVIN2 showed different expression levels in internodes, leaves, and panicles, indicating that their importance in each organ was different. In an in vitro sucrose hydrolysis assay, proteins of both genes heterologously expressed in Pichia pastoris displayed similar enzyme properties including the same optimum reaction pH (5) and similar Km for sucroe (49 mmol L-1 and 45 mmol L-1 for SbVIN1 and SbVIN2, respectively). The optimum reaction temperatures of SbVIN1 and SbVIN2 were 45 degrees C and 65 degrees C, respectively. SbVIN2 showed higher tolerance to high temperature than SbVIN1. We characterized the sequence variation of these two vacuolar invertase genes in a panel of 216 diverse inbred lines of sweet and grain sorghum and performed gene-based association analysis. SbVIN1 showed significant associations with stem traits including stem length, stem diameter, internode number, stem fresh weight, and Brix, as well as grain traits including hundred-grain weight and grain width. Significantly associated variation sites were mainly in 5' upstream and intron regions. SbVIN2 only associated with grain width and stem water-soluble carbohydrates (WSCs) content. We conclude that the vacuolar invertase genes SbVIN1 and SbVIN2 are differently associated with stem and grain traits in sorghum. (c) 2019 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
关键词	Sorghum (Sorghum bicolor) Vacuolar invertase SbVIN1 SbVIN2 Enzymatic properties Association analysis
学科领域	Agronomy ; Plant Sciences
DOI	10.1016/j.cj.2019.06.012
收录类别	SCI
语种	英语
WOS关键词	SOLUBLE ACID INVERTASE ; SUCROSE METABOLISM ; SWEET SORGHUM ; SUGAR ACCUMULATION ; PLANT INVERTASES ; EXPRESSION ; ROLES ; PURIFICATION ; PATTERNS ; DROUGHT
WOS研究方向	Agriculture ; Plant Sciences
WOS记录号	WOS:000522861600011
出版者	KEAI PUBLISHING LTD
文献子类	Article
出版地	BEIJING
EISSN	2214-5141
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31471570, 31461143023] ; Ministry of Science and Technology of the People's Republic of ChinaMinistry of Science and Technology, China [2015BAD15B03]
作者邮箱	hqhao@ibcas.ac.cn
作品OA属性	gold
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21834
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Engn Lab Grass Based Livestock Husb, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Chi, Yunhua,Wilson, Kimani,Liu, Zhiquan,et al. Vacuolar invertase genes SbVIN1 and SbVIN2 are differently associated with stem and grain traits in sorghum (Sorghum bicolor)[J]. CROP JOURNAL,2020,8(2):299-312.
APA	Chi, Yunhua.,Wilson, Kimani.,Liu, Zhiquan.,Wu, Xiaoyuan.,Shang, Li.,...&Hao, Huaiqing.(2020).Vacuolar invertase genes SbVIN1 and SbVIN2 are differently associated with stem and grain traits in sorghum (Sorghum bicolor).CROP JOURNAL,8(2),299-312.
MLA	Chi, Yunhua,et al."Vacuolar invertase genes SbVIN1 and SbVIN2 are differently associated with stem and grain traits in sorghum (Sorghum bicolor)".CROP JOURNAL 8.2(2020):299-312.

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