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Vacuolar invertase genes SbVIN1 and SbVIN2 are differently associated with stem and grain traits in sorghum (Sorghum bicolor)
Chi, Yunhua1; Wilson, Kimani1; Liu, Zhiquan2; Wu, Xiaoyuan; Shang, Li; Zhang, Limin; Jing, Haichun1,2; Hao, Huaiqing
2020
Source PublicationCROP JOURNAL
ISSN2095-5421
Volume8Issue:2Pages:299-312
AbstractIn higher plants, vacuolar invertases play essential roles in sugar metabolism, organ development, and sink strength. In sorghum (Sorghum bicolor), two vacuolar invertase genes, SbVIN1 (Sobic. 004G004800) and SbVIN2 (Sobic. 006G160700) have been reported, but their enzymatic properties and functional differences are largely unknown. We combined molecular, biochemical and genomic approaches to investigate their roles in sorghum stem and grain traits. SbVIN1 and SbVIN2 showed different expression levels in internodes, leaves, and panicles, indicating that their importance in each organ was different. In an in vitro sucrose hydrolysis assay, proteins of both genes heterologously expressed in Pichia pastoris displayed similar enzyme properties including the same optimum reaction pH (5) and similar Km for sucroe (49 mmol L-1 and 45 mmol L-1 for SbVIN1 and SbVIN2, respectively). The optimum reaction temperatures of SbVIN1 and SbVIN2 were 45 degrees C and 65 degrees C, respectively. SbVIN2 showed higher tolerance to high temperature than SbVIN1. We characterized the sequence variation of these two vacuolar invertase genes in a panel of 216 diverse inbred lines of sweet and grain sorghum and performed gene-based association analysis. SbVIN1 showed significant associations with stem traits including stem length, stem diameter, internode number, stem fresh weight, and Brix, as well as grain traits including hundred-grain weight and grain width. Significantly associated variation sites were mainly in 5' upstream and intron regions. SbVIN2 only associated with grain width and stem water-soluble carbohydrates (WSCs) content. We conclude that the vacuolar invertase genes SbVIN1 and SbVIN2 are differently associated with stem and grain traits in sorghum. (c) 2019 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
KeywordSorghum (Sorghum bicolor) Vacuolar invertase SbVIN1 SbVIN2 Enzymatic properties Association analysis
Subject AreaAgronomy ; Plant Sciences
DOI10.1016/j.cj.2019.06.012
Indexed BySCI
Language英语
WOS KeywordSOLUBLE ACID INVERTASE ; SUCROSE METABOLISM ; SWEET SORGHUM ; SUGAR ACCUMULATION ; PLANT INVERTASES ; EXPRESSION ; ROLES ; PURIFICATION ; PATTERNS ; DROUGHT
WOS Research AreaAgriculture ; Plant Sciences
WOS IDWOS:000522861600011
PublisherKEAI PUBLISHING LTD
SubtypeArticle
Publication PlaceBEIJING
EISSN2214-5141
Funding OrganizationNational Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31471570, 31461143023] ; Ministry of Science and Technology of the People's Republic of ChinaMinistry of Science and Technology, China [2015BAD15B03]
Corresponding Author Emailhqhao@ibcas.ac.cn
OAgold
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Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ibcas.ac.cn/handle/2S10CLM1/21834
Collection中科院北方资源植物重点实验室
Affiliation1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Engn Lab Grass Based Livestock Husb, Beijing 100093, Peoples R China
Recommended Citation
GB/T 7714
Chi, Yunhua,Wilson, Kimani,Liu, Zhiquan,et al. Vacuolar invertase genes SbVIN1 and SbVIN2 are differently associated with stem and grain traits in sorghum (Sorghum bicolor)[J]. CROP JOURNAL,2020,8(2):299-312.
APA Chi, Yunhua.,Wilson, Kimani.,Liu, Zhiquan.,Wu, Xiaoyuan.,Shang, Li.,...&Hao, Huaiqing.(2020).Vacuolar invertase genes SbVIN1 and SbVIN2 are differently associated with stem and grain traits in sorghum (Sorghum bicolor).CROP JOURNAL,8(2),299-312.
MLA Chi, Yunhua,et al."Vacuolar invertase genes SbVIN1 and SbVIN2 are differently associated with stem and grain traits in sorghum (Sorghum bicolor)".CROP JOURNAL 8.2(2020):299-312.
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