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| Vacuolar invertase genes SbVIN1 and SbVIN2 are differently associated with stem and grain traits in sorghum (Sorghum bicolor) | |
Chi, Yunhua1; Wilson, Kimani1; Liu, Zhiquan2; Wu, Xiaoyuan; Shang, Li; Zhang, Limin; Jing, Haichun1,2 ; Hao, Huaiqing
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| 2020 | |
| Source Publication | CROP JOURNAL
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| ISSN | 2095-5421 |
| Volume | 8Issue:2Pages:299-312 |
| Abstract | In higher plants, vacuolar invertases play essential roles in sugar metabolism, organ development, and sink strength. In sorghum (Sorghum bicolor), two vacuolar invertase genes, SbVIN1 (Sobic. 004G004800) and SbVIN2 (Sobic. 006G160700) have been reported, but their enzymatic properties and functional differences are largely unknown. We combined molecular, biochemical and genomic approaches to investigate their roles in sorghum stem and grain traits. SbVIN1 and SbVIN2 showed different expression levels in internodes, leaves, and panicles, indicating that their importance in each organ was different. In an in vitro sucrose hydrolysis assay, proteins of both genes heterologously expressed in Pichia pastoris displayed similar enzyme properties including the same optimum reaction pH (5) and similar Km for sucroe (49 mmol L-1 and 45 mmol L-1 for SbVIN1 and SbVIN2, respectively). The optimum reaction temperatures of SbVIN1 and SbVIN2 were 45 degrees C and 65 degrees C, respectively. SbVIN2 showed higher tolerance to high temperature than SbVIN1. We characterized the sequence variation of these two vacuolar invertase genes in a panel of 216 diverse inbred lines of sweet and grain sorghum and performed gene-based association analysis. SbVIN1 showed significant associations with stem traits including stem length, stem diameter, internode number, stem fresh weight, and Brix, as well as grain traits including hundred-grain weight and grain width. Significantly associated variation sites were mainly in 5' upstream and intron regions. SbVIN2 only associated with grain width and stem water-soluble carbohydrates (WSCs) content. We conclude that the vacuolar invertase genes SbVIN1 and SbVIN2 are differently associated with stem and grain traits in sorghum. (c) 2019 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| Keyword | Sorghum (Sorghum bicolor) Vacuolar invertase SbVIN1 SbVIN2 Enzymatic properties Association analysis |
| Subject Area | Agronomy ; Plant Sciences |
| DOI | 10.1016/j.cj.2019.06.012 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | SOLUBLE ACID INVERTASE ; SUCROSE METABOLISM ; SWEET SORGHUM ; SUGAR ACCUMULATION ; PLANT INVERTASES ; EXPRESSION ; ROLES ; PURIFICATION ; PATTERNS ; DROUGHT |
| WOS Research Area | Agriculture ; Plant Sciences |
| WOS ID | WOS:000522861600011 |
| Publisher | KEAI PUBLISHING LTD |
| Subtype | Article |
| Publication Place | BEIJING |
| EISSN | 2214-5141 |
| Funding Organization | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31471570, 31461143023] ; Ministry of Science and Technology of the People's Republic of ChinaMinistry of Science and Technology, China [2015BAD15B03] |
| Corresponding Author Email | hqhao@ibcas.ac.cn |
| OA | gold |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/21834 |
| Collection | 中科院北方资源植物重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Engn Lab Grass Based Livestock Husb, Beijing 100093, Peoples R China |
| Recommended Citation GB/T 7714 | Chi, Yunhua,Wilson, Kimani,Liu, Zhiquan,et al. Vacuolar invertase genes SbVIN1 and SbVIN2 are differently associated with stem and grain traits in sorghum (Sorghum bicolor)[J]. CROP JOURNAL,2020,8(2):299-312. |
| APA | Chi, Yunhua.,Wilson, Kimani.,Liu, Zhiquan.,Wu, Xiaoyuan.,Shang, Li.,...&Hao, Huaiqing.(2020).Vacuolar invertase genes SbVIN1 and SbVIN2 are differently associated with stem and grain traits in sorghum (Sorghum bicolor).CROP JOURNAL,8(2),299-312. |
| MLA | Chi, Yunhua,et al."Vacuolar invertase genes SbVIN1 and SbVIN2 are differently associated with stem and grain traits in sorghum (Sorghum bicolor)".CROP JOURNAL 8.2(2020):299-312. |
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| 1-s2.0-S221451411930（2408KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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