Functional Characterization of Three Novel Genes Encoding Diacylglycerol Acyltransferase (DGAT} from Oil-Rich Tubers of Cyperus esculentus

doi:10.1093/pcp/pcz184

	Functional Characterization of Three Novel Genes Encoding Diacylglycerol Acyltransferase (DGAT} from Oil-Rich Tubers of Cyperus esculentus
	Liu, Dantong 1; Ji, Hongying 1; Yang, Zhenle
	2020
发表期刊	PLANT AND CELL PHYSIOLOGY
ISSN	0032-0781
卷号	61 期号:1 页码:118-129
摘要	Cyperus esculentus is probably the only plant that is known to accumulate large amounts of oil in its tubers. However, the underlying metabolic mechanism and regulatory factors involved in oil synthesis of tubers are still largely unclear. In this study, one gene encoding type I diacylglycerol acyltransferase (DGAT) (CeDGAT1) and two genes encoding type II DGAT (CeDGAT2a and CeDGAT2b) from C esculentus were identified and functionally analyzed. All three DGAT genes were found to be expressed in tuber, root and leaf tissues. CeDGAT1 is highly expressed in roots and leaves, whereas CeDGAT2b is dominantly expressed in tubers. Furthermore, the temporal expression pattern of CeDGAT2b is well coordinated with the oil accumulation in developing tubers. When each CeDGAT was heterologously expressed in triacylglycerol (TAG)-deficient mutant of Saccharomyces cerevisiae, Arabidopsis thaliana wild type or its TAG1 mutant with AtDGAT1 disruption, only CeDGAT2b showed the ability to restore TAG biosynthesis with lipid body formation in yeast mutant, enhance seed oil production of Arabidopsis wild type and rescue multiple seed phenotypes of TAG1 mutant. In addition, CeDGAT2b was shown to have a substrate preference for unsaturated fatty acids toward TAG synthesis. Taken together, our results indicated that CeDGAT2b from C. esculentus is an actively functional protein and is most likely the major contributor to tuber oil biosynthesis containing common fatty acids, in contrast to oil-rich seeds and fruits where DGAT1 plays a more central role than DGAT2 in oil production accumulating normal fatty acids, whereas DGAT2 is a primary regulator for oil synthesis rich in unusual fatty acids.
关键词	Cyperus esculentus Tuber Diacylglycerol acyltransferase Oil Triacylglycerol Fatty acid
学科领域	Plant Sciences ; Cell Biology
DOI	10.1093/pcp/pcz184
收录类别	SCI
语种	英语
WOS关键词	FATTY-ACID PROFILE ; SEED OIL ; TRIACYLGLYCEROL BIOSYNTHESIS ; ARABIDOPSIS ; EXTRACTION ; EXPRESSION ; NUT ; TRANSFORMATION ; TOCOPHEROL ; AVOCADO
WOS研究方向	Plant Sciences ; Cell Biology
WOS记录号	WOS:000522854500011
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1471-9053
资助机构	Natural Science Foundation of Beijing MunicipalityBeijing Natural Science Foundation [5151001] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31371692]
作者邮箱	yangzhl@ibcas.ac.cn
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21836
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, Dantong,Ji, Hongying,Yang, Zhenle. Functional Characterization of Three Novel Genes Encoding Diacylglycerol Acyltransferase (DGAT} from Oil-Rich Tubers of Cyperus esculentus[J]. PLANT AND CELL PHYSIOLOGY,2020,61(1):118-129.
APA	Liu, Dantong,Ji, Hongying,&Yang, Zhenle.(2020).Functional Characterization of Three Novel Genes Encoding Diacylglycerol Acyltransferase (DGAT} from Oil-Rich Tubers of Cyperus esculentus.PLANT AND CELL PHYSIOLOGY,61(1),118-129.
MLA	Liu, Dantong,et al."Functional Characterization of Three Novel Genes Encoding Diacylglycerol Acyltransferase (DGAT} from Oil-Rich Tubers of Cyperus esculentus".PLANT AND CELL PHYSIOLOGY 61.1(2020):118-129.

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