The Hypoxic Proteome and Metabolome of Barley (Hordeum vulgare L.) with and without Phytoglobin Priming

doi:10.3390/ijms21041546

	The Hypoxic Proteome and Metabolome of Barley (Hordeum vulgare L.) with and without Phytoglobin Priming
	Andrzejczak, Olga A.; Havelund, Jesper F.; Wang, Wei-Qing ; Kovalchuk, Sergey 6; Hagensen, Christina E.; Hasler-Sheetal, Harald ; Jensen, Ole N.; Rogowska-Wrzesinska, Adelina ; Moller, Ian Max 4; Hebelstrup, Kim H.
	2020
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷号	21 期号:4
摘要	Overexpression of phytoglobins (formerly plant hemoglobins) increases the survival rate of plant tissues under hypoxia stress by the following two known mechanisms: (1) scavenging of nitric oxide (NO) in the phytoglobin/NO cycle and (2) mimicking ethylene priming to hypoxia when NO scavenging activates transcription factors that are regulated by levels of NO and O-2 in the N-end rule pathway. To map the cellular and metabolic effects of hypoxia in barley (Hordeum vulgare L., cv. Golden Promise), with or without priming to hypoxia, we studied the proteome and metabolome of wild type (WT) and hemoglobin overexpressing (HO) plants in normoxia and after 24 h hypoxia (WT24, HO24). The WT plants were more susceptible to hypoxia than HO plants. The chlorophyll a + b content was lowered by 50% and biomass by 30% in WT24 compared to WT, while HO plants were unaffected. We observed an increase in ROS production during hypoxia treatment in WT seedlings that was not observed in HO seedlings. We identified and quantified 9694 proteins out of which 1107 changed significantly in abundance. Many proteins, such as ion transporters, Ca2+-signal transduction, and proteins related to protein degradation were downregulated in HO plants during hypoxia, but not in WT plants. Changes in the levels of histones indicates that chromatin restructuring plays a role in the priming of hypoxia. We also identified and quantified 1470 metabolites, of which the abundance of >500 changed significantly. In summary the data confirm known mechanisms of hypoxia priming by ethylene priming and N-end rule activation; however, the data also indicate the existence of other mechanisms for hypoxia priming in plants.
关键词	ethylene anaerobiosis hemoglobin histones N-end rule stress priming
学科领域	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
DOI	10.3390/ijms21041546
收录类别	SCI
语种	英语
WOS关键词	NITRIC-OXIDE ; OXIDATIVE STRESS ; LOW-OXYGEN ; MASS-SPECTROMETRY ; ARABIDOPSIS ; PLANTS ; ROOTS ; NO ; BIOSYNTHESIS ; RESPONSES
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS记录号	WOS:000522524400373
出版者	MDPI
文献子类	Article
出版地	BASEL
EISSN	1422-0067
资助机构	Novo Nordisk FoundationNovo Nordisk FoundationNovocure Limited [NNF18OC0052451]
作者邮箱	olga.andrzejczak@mbg.au.dk ; jhav@bmb.sdu.dk ; wwq0814@ibcas.ac.cn ; xerx222@gmail.com ; christinaeh@bmb.sdu.dk ; hasler@sdu.dk ; jenseno@bmb.sdu.dk ; adelinar@bmb.sdu.dk ; ian.max.moller@mbg.au.dk ; kim.hebelstrup@mbg.au.dk
作品OA属性	Green Published, gold
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21837
专题	中科院植物分子生理学重点实验室
作者单位	1.[Andrzejczak, Olga A.; Hebelstrup, Kim H.] Aarhus Univ, Dept Agroecol, Sect Crop Genet & Biotechnol, Forsogsvej 1, DK-4200 Slagelse, Denmark 2.Univ Southern Denmark, Dept Biochem & Mol Biol, Campusvej 55, DK-5230 Odense M, Denmark 3.Univ Southern Denmark, VILLUM Ctr Bioanalyt Sci, Campusvej 55, DK-5230 Odense M, Denmark 4.Univ Southern Denmark, Dept Biol, Nordcee, Campusvej 55, DK-5230 Odense M, Denmark 5.Aarhus Univ, Dept Mol Biol & Genet, Forsogsvej 1, DK-4200 Slagelse, Denmark 6.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 7.RAS, Lab Bioinformat Methods Combinatorial Chem & Biol, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia
推荐引用方式 GB/T 7714	Andrzejczak, Olga A.,Havelund, Jesper F.,Wang, Wei-Qing,et al. The Hypoxic Proteome and Metabolome of Barley (Hordeum vulgare L.) with and without Phytoglobin Priming[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2020,21(4).
APA	Andrzejczak, Olga A..,Havelund, Jesper F..,Wang, Wei-Qing.,Kovalchuk, Sergey.,Hagensen, Christina E..,...&Hebelstrup, Kim H..(2020).The Hypoxic Proteome and Metabolome of Barley (Hordeum vulgare L.) with and without Phytoglobin Priming.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,21(4).
MLA	Andrzejczak, Olga A.,et al."The Hypoxic Proteome and Metabolome of Barley (Hordeum vulgare L.) with and without Phytoglobin Priming".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 21.4(2020).

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