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| Bioactive compounds induced in Physalis angulata L. by methyl-jasmonate: an investigation of compound accumulation patterns and biosynthesis-related candidate genes | |
| Zhan, Xiaori1; Luo, Xiujun1; He, Jinyu1; Zhang, Chengchao1; Liao, Xinyue1; Xu, Xinyun1; Feng, Shangguo1; Yu, Chunna1; Jiang, Zhifang1; Meng, Yijun1; Shen, Chenjia1; Wang, Huizhong1; Lu, Jiangjie1,2 | |
| 2020 | |
| 发表期刊 | PLANT MOLECULAR BIOLOGY
 |
| ISSN | 0167-4412 |
| 卷号 | 103期号:3页码:341-354 |
| 摘要 | Key message We employed both metabolomic and transcriptomic approaches to explore the accumulation patterns of physalins, flavonoids and chlorogenic acid in Physalis angulata and revealed the genes associated with the biosynthesis of bioactive compounds under methyl-jasmonate (MeJA) treatment. Physalis angulata L. is an annual Solanaceae plant with a number of medicinally active compounds. Despite the potential pharmacological benefits of P. angulata, the scarce genomic information regarding this plant has limited the studies on the mechanisms of bioactive compound biosynthesis. To facilitate the basic understanding of the main chemical constituent biosynthesis pathways, we performed both metabolomic and transcriptomic approaches to reveal the genes associated with the biosynthesis of bioactive compounds under methyl-jasmonate (MeJA) treatment. Untargeted metabolome analysis showed that most physalins, flavonoids and chlorogenic acid were significantly upregulated. Targeted HPLC-MS/MS analysis confirmed variations in the contents of two important representative steroid derivatives (physalins B and G), total flavonoids, neochlorogenic acid, and chlorogenic acid between MeJA-treated plants and controls. Transcript levels of a few steroid biosynthesis-, flavonoid biosynthesis-, and chlorogenic acid biosynthesis-related genes were upregulated, providing a potential explanation for MeJA-induced active ingredient synthesis in P. angulata. Systematic correlation analysis identified a number of novel candidate genes associated with bioactive compound biosynthesis. These results may help to elucidate the regulatory mechanism underlying MeJA-induced active compound accumulation and provide several valuable candidate genes for further functional study. |
| 关键词 | Chlorogenic acid Flavonoids Physalins Physalis Methyl-jasmonate (MeJA) Withanolides |
| 学科领域 | Biochemistry & Molecular Biology ; Plant Sciences |
| DOI | 10.1007/s11103-020-00996-y |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | CELL-CYCLE ARREST ; LANOSTEROL SYNTHASE ; IMMUNOSUPPRESSIVE ACTIVITY ; FLAVONOID ACCUMULATION ; CYCLOARTENOL SYNTHASE ; LIQUID-CHROMATOGRAPHY ; CHEMICAL-CONSTITUENTS ; SQUALENE EPOXIDASE ; CHALCONE SYNTHASE ; VAR. FRANCHETII |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS记录号 | WOS:000522183500001 |
| 出版者 | SPRINGER |
| 文献子类 | Article |
| 出版地 | DORDRECHT |
| EISSN | 1573-5028 |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31470407, 31970346] ; Major Increase or Decrease Program In The Central Finance Level [2060302] |
| 作者邮箱 | whz62@163.com ; lujj@hznu.edu.cn |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/21843 |
| 专题 | 系统与进化植物学国家重点实验室 |
| 作者单位 | 1.Hangzhou Normal Univ, Coll Life & Environm Sci, Hangzhou 310036, Peoples R China 2.Hangzhou Normal Univ, Zhejiang Prov Key Lab Genet Improvement & Qual Co, Hangzhou 310036, Peoples R China 3.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Nanxincun 20, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhan, Xiaori,Luo, Xiujun,He, Jinyu,et al. Bioactive compounds induced in Physalis angulata L. by methyl-jasmonate: an investigation of compound accumulation patterns and biosynthesis-related candidate genes[J]. PLANT MOLECULAR BIOLOGY,2020,103(3):341-354. |
| APA | Zhan, Xiaori.,Luo, Xiujun.,He, Jinyu.,Zhang, Chengchao.,Liao, Xinyue.,...&Lu, Jiangjie.(2020).Bioactive compounds induced in Physalis angulata L. by methyl-jasmonate: an investigation of compound accumulation patterns and biosynthesis-related candidate genes.PLANT MOLECULAR BIOLOGY,103(3),341-354. |
| MLA | Zhan, Xiaori,et al."Bioactive compounds induced in Physalis angulata L. by methyl-jasmonate: an investigation of compound accumulation patterns and biosynthesis-related candidate genes".PLANT MOLECULAR BIOLOGY 103.3(2020):341-354. |
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| Zhan-2020-Bioactive （7260KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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