Liquid-Liquid Phase Transition Drives Intra-chloroplast Cargo Sorting

doi:10.1016/j.cell.2020.02.045

IB-CAS > 中科院光生物学重点实验室

	Liquid-Liquid Phase Transition Drives Intra-chloroplast Cargo Sorting
	Ouyang, Min 2; Li, Xiaoyi ; Zhang, Jing 7; Feng, Peiqiang ; Pu, Hua ; Kong, Lingxi 7; Bai, Zechen 7; Rong, Liwei 7; Xu, Xiumei 1; Chi, Wei 7; Wang, Qiang 1; Chen, Fan 5; Lu, Congming 6; Shen, Jianren3,4 ; Zhang, Lixin1
	2020
发表期刊	CELL
ISSN	0092-8674
卷号	180 期号:6 页码:1144-1159
摘要	In eukaryotic cells, organelle biogenesis is pivotal for cellular function and cell survival. Chloroplasts are unique organelles with a complex internal membrane network. The mechanisms of the migration of imported nuclear-encoded chloroplast proteins across the crowded stroma to thylakoid membranes are less understood. Here, we identified two Arabidopsis ankyrin-repeat proteins, STT1 and STT2, that specifically mediate sorting of chloroplast twin arginine translocation (cpTat) pathway proteins to thylakoid membranes. STT1 and STT2 form a unique heterodimer through interaction of their C-terminal ankyrin domains. Binding of cpTat substrate by N-terminal intrinsically disordered regions of STT complex induces liquid-liquid phase separation. The multivalent nature of STT oligomer is critical for phase separation. STT-Hcf106 interactions reverse phase separation and facilitate cargo targeting and translocation across thylakoid membranes. Thus, the formation of phase-separated droplets emerges as a novel mechanism of intra-chloroplast cargo sorting. Our findings highlight a conserved mechanism of phase separation in regulating organelle biogenesis.
学科领域	Biochemistry & Molecular Biology ; Cell Biology
DOI	10.1016/j.cell.2020.02.045
收录类别	SCI
语种	英语
WOS关键词	SIGNAL RECOGNITION PARTICLE ; PROTEIN-TRANSPORT ; PRECURSOR PROTEINS ; PEPTIDE-BINDING ; ANKYRIN REPEAT ; TRANSLOCATION ; SEPARATION ; IMPORT ; PLASTIDS ; FEATURES
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
WOS记录号	WOS:000520925300012
出版者	CELL PRESS
文献子类	Article
出版地	CAMBRIDGE
EISSN	1097-4172
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31471267, 31130059] ; State Key Basic Research and Development Plan of ChinaNational Basic Research Program of China [2015CB150100] ; National Key Research and Development Program of China [2017YFA0503700] ; Outstanding Talents Fund of Henan University, China ; State Key Laboratory of Biocatalysis and Enzyme Engineering of Hubei University, China ; Youth Innovation Promotion Association CAS, China [2013059]
作者邮箱	zhanglixin@henu.edu.cn
作品OA属性	Bronze
引用统计	被引频次：59[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21851
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Henan Univ, Sch Life Sci, State Key Lab Crop Stress Adaptat & Improvement, 85 Minglun St, Kaifeng 475001, Peoples R China 3.Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430062, Peoples R China 4.Okayama Univ, Res Inst Interdisciplinary Sci, Okayama 7008530, Japan 5.Okayama Univ, Grad Sch Nat Sci & Technol, Okayama 7008530, Japan 6.Chinese Acad Sci, State Key Lab Mol Dev Biol, Beijing 100086, Peoples R China 7.Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China 8.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Ouyang, Min,Li, Xiaoyi,Zhang, Jing,et al. Liquid-Liquid Phase Transition Drives Intra-chloroplast Cargo Sorting[J]. CELL,2020,180(6):1144-1159.
APA	Ouyang, Min.,Li, Xiaoyi.,Zhang, Jing.,Feng, Peiqiang.,Pu, Hua.,...&Zhang, Lixin.(2020).Liquid-Liquid Phase Transition Drives Intra-chloroplast Cargo Sorting.CELL,180(6),1144-1159.
MLA	Ouyang, Min,et al."Liquid-Liquid Phase Transition Drives Intra-chloroplast Cargo Sorting".CELL 180.6(2020):1144-1159.

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