Actin Is Required for Cellular Development and Virulence of Botrytis cinerea via the Mediation of Secretory Proteins

doi:10.1128/mSystems.00732-19

	Actin Is Required for Cellular Development and Virulence of Botrytis cinerea via the Mediation of Secretory Proteins
	Li, Hua 1; Zhang, Zhanquan 2; Qin, Guozheng2 ; He, Chang 1; Li, Boqiang 2; Tian, Shiping1,2
	2020
发表期刊	MSYSTEMS
ISSN	2379-5077
卷号	5 期号:1
摘要	Actin is a vital component of the cytoskeleton of living cells and is involved in several complex processes. However, its functions in plant-pathogenic fungi are largely unknown. In this paper, we found that deletion of the Botrytis cinerea actin gene bcactA reduced growth and sporulation of B. cinerea and lowered virulence. Based on iTRAQ (isobaric tags for relative and absolute quantification)based proteomic analysis, we compared changes of the secretome in Delta bcactA and wild-type strains. A total of 40 proteins exhibited significant differences in abundance in Delta bcactA mutants compared with the wild type. These proteins included 11 down-accumulated cell wall-degrading enzymes (CWDEs). Among them, two CWDEs, cellobiohydrolase (BcCBH) and beta-endoglucanase (BcEG), were found to contribute to the virulence of B. cinerea, indicating that bcactA plays a crucial role in regulating the secretion of extracellular virulence factors. These findings unveil previously unknown functions of BcactA to mediate the virulence of B. cinerea and provide new mechanistic insights into the role of BcactA in the complex pathogenesis of B. cinerea. IMPORTANCE The cytoskeleton is an important network that exists in cells of all domains of life. In eukaryotic cells, actin is a vital component of the cytoskeleton. Here, we report that BcactA, an actin protein in B. cinerea, can affect the growth, sporulation, and virulence of B. cinerea. Furthermore, iTRAQ-based proteomic analysis showed that BcactA affects the abundance of 40 extracellular proteins, including 11 down-accumulated CWDEs. Among them, two CWDEs, cellobiohydrolase (BcCBH) and beta-endoglucanase (BcEG), contributed to the virulence of B. cinerea, indicating that bcactA plays a crucial role in regulating extracellular virulence factors. These findings unveil previously unknown functions of BcactA in mediating growth, sporulation, and virulence of B. cinerea.
关键词	actin Botrytis cinerea pathogenesis secretome
学科领域	Microbiology
DOI	10.1128/mSystems.00732-19
收录类别	SCI
语种	英语
WOS关键词	PROTEOMIC ANALYSIS ; NADPH-OXIDASE ; GROWTH ; CYTOSKELETON ; ISOVARIANTS ; MECHANISMS ; REVEALS ; ENZYMES ; FUNGUS
WOS研究方向	Microbiology
WOS记录号	WOS:000518855000024
出版者	AMER SOC MICROBIOLOGY
文献子类	Article
出版地	WASHINGTON
资助机构	Key Program of National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31930086, 31530057] ; National Key R&D Program of China [2016YFD0400902] ; Youth Innovation Promotion Association CAS [2018107]
作者邮箱	tsp@ibcas.ac.cn
引用统计	被引频次：30[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21868
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Minist Agr China, Key Lab Postharvest Handling Fruits, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Li, Hua,Zhang, Zhanquan,Qin, Guozheng,et al. Actin Is Required for Cellular Development and Virulence of Botrytis cinerea via the Mediation of Secretory Proteins[J]. MSYSTEMS,2020,5(1).
APA	Li, Hua,Zhang, Zhanquan,Qin, Guozheng,He, Chang,Li, Boqiang,&Tian, Shiping.(2020).Actin Is Required for Cellular Development and Virulence of Botrytis cinerea via the Mediation of Secretory Proteins.MSYSTEMS,5(1).
MLA	Li, Hua,et al."Actin Is Required for Cellular Development and Virulence of Botrytis cinerea via the Mediation of Secretory Proteins".MSYSTEMS 5.1(2020).

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