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Amino acid composition, protein content and accurate nitrogen-to-protein conversion factor for sheepgrass (Leymus chinensis) | |
Zhang, Qingfen1; Qi, Dongmei; Dong, Xiaobing; Li, Xiaoxia; Cheng, Liqin; Liu, Hui; Chen, Shuangyan; Rajora, Om P.; Li, Xiu-Qing; Liu, Gongshe | |
2020 | |
发表期刊 | BOTANY |
ISSN | 1916-2790 |
卷号 | 98期号:3页码:137-146 |
摘要 | The protein content of plants is commonly estimated by multiplying the total nitrogen content (Kjeldahl; KN) by a nitrogen-to-protein conversion factor of 6.25. This method is based on the incorrect assumption that all nitrogen in the ammonia/ammonium and organic substances in plants is protein nitrogen, usually resulting in overestimation of protein content. We have examined amino acid composition, amino acid nitrogen, total nitrogen (KN), and actual protein content (AP) determined from amino acid residues in 16 accessions of perennial sheepgrass (Leymus chinensis (Trin.) Tzvelev). We determined a new nitrogen-to-protein conversion factor, k(p), as the ratio of AP to KN, and applied this factor to estimate the total protein content (TP) as KN x k(p). The non-protein nitrogen accounted for 40.5% to 62.4% of the total nitrogen. The average k(p) value was 3.17 overall, 3.20 in the accessions sampled at the jointing stage, and 3.15 in the accessions sampled at the flowering stage. The TP, calculated as KN x 3.17, was about half that of crude protein contents, calculated as KN x 6.25. Our study suggests that the AP-based k(p) of 3.17 can be used to more accurately estimate the total protein content in sheepgrass. |
关键词 | sheepgrass (Leymus chinensis) amino acid composition protein content nitrogen-to-protein conversion factor |
学科领域 | Plant Sciences |
DOI | 10.1139/cjb-2019-0082 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | COMMON ; PROLINE |
WOS研究方向 | Plant Sciences |
WOS记录号 | WOS:000518797200002 |
出版者 | CANADIAN SCIENCE PUBLISHING |
文献子类 | Article |
出版地 | OTTAWA |
EISSN | 1916-2804 |
资助机构 | National Key R&D Program of China [2018YFD1001000, 2016YFC0500700] ; Science and Technology Major Project of Inner Mongolia Autonomous Region of China ; Kulun Banner Science and Technology Poverty Alleviation Project of Chinese Academy of Sciences |
作者邮箱 | sychen@ibcas.ac.cn ; Om.Rajora@unb.ca ; Xiu-Qing.li@Canada.ca ; liugs@ibcas.ac.cn |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/21869 |
专题 | 中科院北方资源植物重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Rajora, Om P.] Univ New Brunswick, Fac Forestry & Environm Management, 28 Dineen Dr, Fredericton, NB E3B 5A3, Canada 4.Agr & Agri Food Canada, Fredericton Res & Dev Ctr, Fredericton, NB E3B 4Z7, Canada |
推荐引用方式 GB/T 7714 | Zhang, Qingfen,Qi, Dongmei,Dong, Xiaobing,et al. Amino acid composition, protein content and accurate nitrogen-to-protein conversion factor for sheepgrass (Leymus chinensis)[J]. BOTANY,2020,98(3):137-146. |
APA | Zhang, Qingfen.,Qi, Dongmei.,Dong, Xiaobing.,Li, Xiaoxia.,Cheng, Liqin.,...&Liu, Gongshe.(2020).Amino acid composition, protein content and accurate nitrogen-to-protein conversion factor for sheepgrass (Leymus chinensis).BOTANY,98(3),137-146. |
MLA | Zhang, Qingfen,et al."Amino acid composition, protein content and accurate nitrogen-to-protein conversion factor for sheepgrass (Leymus chinensis)".BOTANY 98.3(2020):137-146. |
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cjb-2019-0082.pdf(2999KB) | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 |
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