A role of age-dependent DNA methylation reprogramming in regulating the regeneration capacity of Boea hygrometrica leaves

doi:10.1007/s10142-019-00701-3

	A role of age-dependent DNA methylation reprogramming in regulating the regeneration capacity of Boea hygrometrica leaves
	Sun, Run-Ze ; Zuo, En-Hui ; Qi, Jin-Feng ; Liu, Yang ; Lin, Chih-Ta ; Deng, Xin5
	2020
发表期刊	FUNCTIONAL & INTEGRATIVE GENOMICS
ISSN	1438-793X
卷号	20 期号:1 页码:133-149
摘要	Plants can regenerate new individuals under appropriate culture conditions. Although the molecular basis of shoot regeneration has steadily been unraveled, the role of age-dependent DNA methylation status in the regulation of explant regeneration remains practically unknown. Here, we established an effective auxin/cytokinin-induced shoot regeneration system for the resurrection plant Boea hygrometrica via direct organogenesis and observed that regeneration was postponed with increasing age of donor plants. Global transcriptome analysis revealed significant upregulation of genes required for hormone signaling and phenylpropanoid biosynthesis and downregulation of photosynthetic genes during regeneration. Transcriptional changes in the positive/negative regulators and cell wall-related proteins involved in plant regeneration, such as ELONGATED HYPOCOTYL5 (HY5), LATERAL ORGAN BOUNDARIES DOMAIN, SHOOT-MERISTEMLESS, and WUSCHEL, were associated with the regeneration process. Comparison of DNA methylation profiling between leaves from young seedlings (YL) and mature plants (ML) revealed increased asymmetrical methylation in ML, which was predominantly distributed in promoter regions of genes, such as HY5 and a member of ABA-responsive element (ABRE) binding protein/ABRE binding factor, as well as genes encoding glycine-rich cell wall structural protein, CENTRORADIALIS-like protein, and beta-glucosidase 40-like essential for shoot meristem and cell wall architecture. Their opposite transcription response in ML explants during regeneration compared with those from YL demonstrated the putative involvement of DNA methylation in regeneration. Moreover, a significant lower expression of DNA glycosylase-lyase required for DNA demethylation in ML was coincident with its postponed regeneration compared with those in YL. Taken together, our results suggest a role of promoter demethylation in B. hygrometrica regeneration.
关键词	Shoot regeneration Donor plant age DNA methylome Transcriptome Resurrection plant Boea hygrometrica
学科领域	Genetics & Heredity
DOI	10.1007/s10142-019-00701-3
收录类别	SCI
语种	英语
WOS关键词	PLANT-REGENERATION ; SHOOT REGENERATION ; IN-VITRO ; ROOT DEVELOPMENT ; TRANSCRIPTIONAL ACTIVATION ; SOMATIC EMBRYOGENESIS ; CALLUS INITIATION ; GROWTH-REGULATORS ; ARABIDOPSIS ; GENE
WOS研究方向	Genetics & Heredity
WOS记录号	WOS:000518760700010
出版者	SPRINGER HEIDELBERG
文献子类	Article
出版地	HEIDELBERG
EISSN	1438-7948
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31770293, 31470361]
作者邮箱	deng@ibcas.ac.cn
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21870
专题	中科院北方资源植物重点实验室
作者单位	1.[Sun, Run-Ze 2.Zuo, En-Hui 3.Qi, Jin-Feng 4.Liu, Yang 5.Lin, Chih-Ta 6.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Sun, Run-Ze,Zuo, En-Hui,Qi, Jin-Feng,et al. A role of age-dependent DNA methylation reprogramming in regulating the regeneration capacity of Boea hygrometrica leaves[J]. FUNCTIONAL & INTEGRATIVE GENOMICS,2020,20(1):133-149.
APA	Sun, Run-Ze,Zuo, En-Hui,Qi, Jin-Feng,Liu, Yang,Lin, Chih-Ta,&Deng, Xin.(2020).A role of age-dependent DNA methylation reprogramming in regulating the regeneration capacity of Boea hygrometrica leaves.FUNCTIONAL & INTEGRATIVE GENOMICS,20(1),133-149.
MLA	Sun, Run-Ze,et al."A role of age-dependent DNA methylation reprogramming in regulating the regeneration capacity of Boea hygrometrica leaves".FUNCTIONAL & INTEGRATIVE GENOMICS 20.1(2020):133-149.

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