Nuclear Localized O-Fucosyltransferase SPY Facilitates PRR5 Proteolysis to Fine-Tune the Pace of Arabidopsis Circadian Clock

doi:10.1016/j.molp.2019.12.013

	Nuclear Localized O-Fucosyltransferase SPY Facilitates PRR5 Proteolysis to Fine-Tune the Pace of Arabidopsis Circadian Clock
	Wang, Yan 1; He, Yuqing 1; Su, Chen 1; Zentella, Rodolfo ; Sun, Tai-ping ; Wang, Lei1
	2020
发表期刊	MOLECULAR PLANT
ISSN	1674-2052
卷号	13 期号:3 页码:446-458
摘要	Post-translational modifications play essential roles in finely modulating eukaryotic circadian clock systems. In plants, the effects of O-glycosylation on the circadian clock and the underlying mechanisms remain largely unknown. The O-fucosyltransferase SPINDLY (SPY) and the O-GlcNAc transferase SECRET AGENT (SEC) are two prominent O-glycosylation enzymes in higher plants, with both overlapped and unique functions in plant growth and development. Unlike the critical role of O-GlcNAc in regulating the animal circadian clock, here we report that nuclear-localized SPY, but not SEC, specifically modulates the pace of the Arabidopsis circadian clock. By identifying the interactome of SPY, we identified PSEUDORESPONSE REGULATOR 5 (PRR5), one of the core circadian clock components, as a new SPY-interacting protein. PRR5 can be O-fucosylated by SPY in planta, while point mutation in the catalytic domain of SPY abolishes the O-fucosylation of PRR5. The protein abundance of PRR5 is strongly increased in spy mutants, while the degradation rate of PRR5 is much reduced, suggesting that PRR5 proteolysis is promoted by SPY-mediated O-fucosylation. Moreover, multiple lines of genetic evidence indicate that PRR5 is a major downstream target of SPY to specifically mediate its modulation of the circadian clock. Collectively, our findings provide novel insights into the specific role of the O-fucosyltransferase activity of SPY in modulating the circadian clock and implicate that O-glycosylation might play an evolutionarily conserved role in modulating the circadian clock system, via O-GlcNAcylation in mammals, but via O-fucosylation in higher plants.
关键词	circadian clock O-fucosyltransferase SPY proteolysis PRR5
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
DOI	10.1016/j.molp.2019.12.013
收录类别	SCI
语种	英语
WOS关键词	PSEUDO-RESPONSE REGULATORS ; GIBBERELLIN-RESPONSE ; SECRET-AGENT ; PHOSPHORYLATION ; REPRESSOR ; PLANT ; GLCNACYLATION ; EXPRESSION ; COMPLEX ; PROTEIN
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
WOS记录号	WOS:000518446000010
出版者	CELL PRESS
文献子类	Article
出版地	CAMBRIDGE
EISSN	1752-9867
资助机构	Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDB27030206] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31770287] ; National Key Research and Development Program of China [2016YFD0100600] ; National Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R01 GM100051]
作者邮箱	wanglei@ibcas.ac.cn
作品OA属性	Green Accepted, Bronze
引用统计	被引频次：37[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21877
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Duke Univ, Dept Biol, Durham, NC 27708 USA
推荐引用方式 GB/T 7714	Wang, Yan,He, Yuqing,Su, Chen,et al. Nuclear Localized O-Fucosyltransferase SPY Facilitates PRR5 Proteolysis to Fine-Tune the Pace of Arabidopsis Circadian Clock[J]. MOLECULAR PLANT,2020,13(3):446-458.
APA	Wang, Yan,He, Yuqing,Su, Chen,Zentella, Rodolfo,Sun, Tai-ping,&Wang, Lei.(2020).Nuclear Localized O-Fucosyltransferase SPY Facilitates PRR5 Proteolysis to Fine-Tune the Pace of Arabidopsis Circadian Clock.MOLECULAR PLANT,13(3),446-458.
MLA	Wang, Yan,et al."Nuclear Localized O-Fucosyltransferase SPY Facilitates PRR5 Proteolysis to Fine-Tune the Pace of Arabidopsis Circadian Clock".MOLECULAR PLANT 13.3(2020):446-458.

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