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| Nuclear Localized O-Fucosyltransferase SPY Facilitates PRR5 Proteolysis to Fine-Tune the Pace of Arabidopsis Circadian Clock | |
Wang, Yan1; He, Yuqing1; Su, Chen1; Zentella, Rodolfo; Sun, Tai-ping; Wang, Lei1
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| 2020 | |
| Source Publication | MOLECULAR PLANT
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| ISSN | 1674-2052 |
| Volume | 13Issue:3Pages:446-458 |
| Abstract | Post-translational modifications play essential roles in finely modulating eukaryotic circadian clock systems. In plants, the effects of O-glycosylation on the circadian clock and the underlying mechanisms remain largely unknown. The O-fucosyltransferase SPINDLY (SPY) and the O-GlcNAc transferase SECRET AGENT (SEC) are two prominent O-glycosylation enzymes in higher plants, with both overlapped and unique functions in plant growth and development. Unlike the critical role of O-GlcNAc in regulating the animal circadian clock, here we report that nuclear-localized SPY, but not SEC, specifically modulates the pace of the Arabidopsis circadian clock. By identifying the interactome of SPY, we identified PSEUDORESPONSE REGULATOR 5 (PRR5), one of the core circadian clock components, as a new SPY-interacting protein. PRR5 can be O-fucosylated by SPY in planta, while point mutation in the catalytic domain of SPY abolishes the O-fucosylation of PRR5. The protein abundance of PRR5 is strongly increased in spy mutants, while the degradation rate of PRR5 is much reduced, suggesting that PRR5 proteolysis is promoted by SPY-mediated O-fucosylation. Moreover, multiple lines of genetic evidence indicate that PRR5 is a major downstream target of SPY to specifically mediate its modulation of the circadian clock. Collectively, our findings provide novel insights into the specific role of the O-fucosyltransferase activity of SPY in modulating the circadian clock and implicate that O-glycosylation might play an evolutionarily conserved role in modulating the circadian clock system, via O-GlcNAcylation in mammals, but via O-fucosylation in higher plants. |
| Keyword | circadian clock O-fucosyltransferase SPY proteolysis PRR5 |
| Subject Area | Biochemistry & Molecular Biology ; Plant Sciences |
| DOI | 10.1016/j.molp.2019.12.013 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | PSEUDO-RESPONSE REGULATORS ; GIBBERELLIN-RESPONSE ; SECRET-AGENT ; PHOSPHORYLATION ; REPRESSOR ; PLANT ; GLCNACYLATION ; EXPRESSION ; COMPLEX ; PROTEIN |
| WOS Research Area | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS ID | WOS:000518446000010 |
| Publisher | CELL PRESS |
| Subtype | Article |
| Publication Place | CAMBRIDGE |
| EISSN | 1752-9867 |
| Funding Organization | Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDB27030206] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31770287] ; National Key Research and Development Program of China [2016YFD0100600] ; National Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R01 GM100051] |
| Corresponding Author Email | wanglei@ibcas.ac.cn |
| OA | Green Accepted, Bronze |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/21877 |
| Collection | 中科院植物分子生理学重点实验室 |
| Affiliation | 1.Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Duke Univ, Dept Biol, Durham, NC 27708 USA |
| Recommended Citation GB/T 7714 | Wang, Yan,He, Yuqing,Su, Chen,et al. Nuclear Localized O-Fucosyltransferase SPY Facilitates PRR5 Proteolysis to Fine-Tune the Pace of Arabidopsis Circadian Clock[J]. MOLECULAR PLANT,2020,13(3):446-458. |
| APA | Wang, Yan,He, Yuqing,Su, Chen,Zentella, Rodolfo,Sun, Tai-ping,&Wang, Lei.(2020).Nuclear Localized O-Fucosyltransferase SPY Facilitates PRR5 Proteolysis to Fine-Tune the Pace of Arabidopsis Circadian Clock.MOLECULAR PLANT,13(3),446-458. |
| MLA | Wang, Yan,et al."Nuclear Localized O-Fucosyltransferase SPY Facilitates PRR5 Proteolysis to Fine-Tune the Pace of Arabidopsis Circadian Clock".MOLECULAR PLANT 13.3(2020):446-458. |
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| Wang-2020-Nuclear Lo（2485KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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