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PHYTOCHROME-INTERACTING FACTORS Interact with the ABA Receptors PYL8 and PYL9 to Orchestrate ABA Signaling in Darkness
Qi, Lijuan; Liu, Shan; Li, Cong; Fu, Jingying; Jing, Yanjun1; Cheng, Jinkui; Li, Hong; Zhang, Dun; Wang, Xiaoji; Dong, Xiaojing; Han, Run; Li, Bosheng2; Zhang, Yu3,4; Li, Zhen; Terzaghi, William5; Song, Chun-Peng; Lin, Rongcheng1; Gong, Zhizhong; Li, Jigang
Source PublicationMOLECULAR PLANT
AbstractPHYTOCHROME-INTERACTING FACTORS (PIFs) are a group of basic helix-loop-helix transcription factors that can physically interact with photoreceptors, including phytochromes and cryptochromes. It was previously demonstrated that PIFs accumulated in darkness and repressed seedling photomorphogenesis, and that PIFs linked different photosensory and hormonal pathways to control plant growth and development. In this study, we show that PIFs positively regulate the ABA signaling pathway during the seedling stage specifically in darkness. We found that PIFs positively regulate ABI5 transcript and protein levels in darkness in response to exogenous ABA treatment by binding directly to the G-box motifs in the ABI5 promoter. Consistently, PIFs and the G-box motifs in theABI5 promoter determineABI5 expression in darkness, and overexpression of ABI5 could rescue the ABA-insensitive phenotypes of pifq mutants in the dark. Moreover, we discovered that PIFs can physically interact with the ABA receptors PYL8 and PYL9, and that this interaction is not regulated by ABA. Further analyses showed that PYL8 and PYL9 promote PIF4 protein accumulation in the dark and enhance PIF4 binding to the ABI5 promoter, but negatively regulate PIF4-mediated ABI5 activation. Taken together, our data demonstrate that PIFs interact with ABA receptors to orchestrate ABA signaling in darkness by controlling ABI5 expression, providing new insights into the pivotal roles of PIFs as signal integrators in regulating plant growth and development.
KeywordArabidopsis transcription factor PIFs ABA ABI5 darkness
Subject AreaBiochemistry & Molecular Biology ; Plant Sciences
Indexed BySCI
WOS Research AreaBiochemistry & Molecular Biology ; Plant Sciences
WOS IDWOS:000518446000008
Publication PlaceCAMBRIDGE
Funding OrganizationNational Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31970262, 31770321] ; Ministry of Agriculture of ChinaMinistry of Agriculture, China [2019ZX08010003-002-005] ; Beijing Outstanding University Discipline Program ; Recruitment Program of Global Youth Experts of China
Corresponding Author Emailjigangli@cau.edu.cn
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Cited Times:44[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Affiliation1.China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing 100193, Peoples R China
2.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China
3.Southern Univ Sci & Technol, Inst Plant & Food Sci, Dept Biol, Shenzhen 518055, Guangdong, Peoples R China
4.Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA
5.ARS, Plant Gene Express Ctr, USDA, Albany, CA 94710 USA
6.Wilkes Univ, Dept Biol, Wilkes Barre, PA 18766 USA
7.Henan Univ, Collaborat Innovat Ctr Crop Stress Biol, Inst Plant Stress Biol, Kaifeng 475001, Peoples R China
Recommended Citation
GB/T 7714
Qi, Lijuan,Liu, Shan,Li, Cong,et al. PHYTOCHROME-INTERACTING FACTORS Interact with the ABA Receptors PYL8 and PYL9 to Orchestrate ABA Signaling in Darkness[J]. MOLECULAR PLANT,2020,13(3):414-430.
APA Qi, Lijuan.,Liu, Shan.,Li, Cong.,Fu, Jingying.,Jing, Yanjun.,...&Li, Jigang.(2020).PHYTOCHROME-INTERACTING FACTORS Interact with the ABA Receptors PYL8 and PYL9 to Orchestrate ABA Signaling in Darkness.MOLECULAR PLANT,13(3),414-430.
MLA Qi, Lijuan,et al."PHYTOCHROME-INTERACTING FACTORS Interact with the ABA Receptors PYL8 and PYL9 to Orchestrate ABA Signaling in Darkness".MOLECULAR PLANT 13.3(2020):414-430.
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