Function of PsbO-Asp158 in photosystem II: effects of mutation of this residue on the binding of PsbO and function of PSII in Thermosynechococcus vulcanus

doi:10.1007/s11120-020-00715-0

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	Function of PsbO-Asp158 in photosystem II: effects of mutation of this residue on the binding of PsbO and function of PSII in Thermosynechococcus vulcanus
	Zhu, Qingjun 1; Yang, Yanyan ; Xiao, Yanan 1; Wang, Wenda 1; Kuang, Tingyun ; Shen, Jian-Ren; Han, Guangye
	2020
发表期刊	PHOTOSYNTHESIS RESEARCH
ISSN	0166-8595
卷号	146 期号:1-3 页码:29-40
摘要	PsbO-D158 is a highly conserved residue of the PsbO protein in photosystem II (PSII), and participates in one of the hydrogen-bonding networks connecting the manganese cluster with the lumenal surface. In order to examine the role of PsbO-D158, we mutated it to E, N or K in Thermosynechococcus vulcanus and characterized photosynthetic properties of the mutants obtained. The growth rates of these three mutants were similar to that of the wild type, whereas the oxygen-evolving activity of the three mutant cells decreased to 60-64% of the wild type. Fluorescence kinetics showed that the mutations did not affect the electron transfer from Q(A) to Q(B), but slightly affected the donor side of PSII. Moreover, all of the three mutant cells were more sensitive to high light and became slower to recover from photoinhibition. In the isolated thylakoid membranes from the three mutants, the PsbU subunit was lost and the oxygen-evolving activity was reduced to a lower level compared to that in the respective cells. PSII complexes isolated from these mutants showed no oxygen-evolving activity, which was found to be due to large or complete loss of PsbO, PsbV and PsbU during the process of purification. Moreover, PSII cores purified from the three mutants contained Psb27, an assembly co-factor of PSII. These results suggest that PsbO-D158 is required for the proper binding of the three extrinsic proteins to PSII and plays an important role in maintaining the optimal oxygen-evolving activity, and its mutation caused incomplete assembly of the PSII complex.
关键词	PsbO Site-directed mutagenesis Photosystem II Oxygen evolution Thermosynechococcus vulcanus
学科领域	Plant Sciences
DOI	10.1007/s11120-020-00715-0
收录类别	SCI
语种	英语
WOS关键词	MANGANESE-STABILIZING PROTEIN ; SITE-DIRECTED MUTANTS ; EXTRINSIC PROTEINS ; OXYGEN-EVOLUTION ; DELETION MUTAGENESIS ; WATER OXIDATION ; CLUSTER ; SUBUNIT ; POLYPEPTIDE ; ELONGATUS
WOS研究方向	Plant Sciences
WOS记录号	WOS:000518147700001
出版者	SPRINGER
Special Issue	SI
文献子类	Article
出版地	DORDRECHT
EISSN	1573-5079
资助机构	National Key R&D Program of China [2017YFA0503700] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31470339] ; Strategic Priority Research Program of CAS [XDB17000000] ; Key Research Program of Frontier Sciences, CAS [QYZDY-SSW-SMC003]
作者邮箱	shen@cc.okayama-u.ac.jp ; hanguangye@ibcas.ac.cn
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21882
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Photosynth Res Ctr, Key Lab Photobiol, Inst Bot, 20 Nanxincun, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Yuquan Rd, Beijing 100049, Peoples R China 3.Okayama Univ, Res Inst Interdisciplinary Sci, Grad Sch Nat Sci & Technol, Tsushima Naka 3-1-1, Okayama 7008530, Japan
推荐引用方式 GB/T 7714	Zhu, Qingjun,Yang, Yanyan,Xiao, Yanan,et al. Function of PsbO-Asp158 in photosystem II: effects of mutation of this residue on the binding of PsbO and function of PSII in Thermosynechococcus vulcanus[J]. PHOTOSYNTHESIS RESEARCH,2020,146(1-3):29-40.
APA	Zhu, Qingjun.,Yang, Yanyan.,Xiao, Yanan.,Wang, Wenda.,Kuang, Tingyun.,...&Han, Guangye.(2020).Function of PsbO-Asp158 in photosystem II: effects of mutation of this residue on the binding of PsbO and function of PSII in Thermosynechococcus vulcanus.PHOTOSYNTHESIS RESEARCH,146(1-3),29-40.
MLA	Zhu, Qingjun,et al."Function of PsbO-Asp158 in photosystem II: effects of mutation of this residue on the binding of PsbO and function of PSII in Thermosynechococcus vulcanus".PHOTOSYNTHESIS RESEARCH 146.1-3(2020):29-40.

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