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| Function of PsbO-Asp158 in photosystem II: effects of mutation of this residue on the binding of PsbO and function of PSII in Thermosynechococcus vulcanus | |
Zhu, Qingjun1; Yang, Yanyan; Xiao, Yanan1; Wang, Wenda1; Kuang, Tingyun; Shen, Jian-Ren ; Han, Guangye
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| 2020 | |
| Source Publication | PHOTOSYNTHESIS RESEARCH
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| ISSN | 0166-8595 |
| Volume | 146Issue:1-3Pages:29-40 |
| Abstract | PsbO-D158 is a highly conserved residue of the PsbO protein in photosystem II (PSII), and participates in one of the hydrogen-bonding networks connecting the manganese cluster with the lumenal surface. In order to examine the role of PsbO-D158, we mutated it to E, N or K in Thermosynechococcus vulcanus and characterized photosynthetic properties of the mutants obtained. The growth rates of these three mutants were similar to that of the wild type, whereas the oxygen-evolving activity of the three mutant cells decreased to 60-64% of the wild type. Fluorescence kinetics showed that the mutations did not affect the electron transfer from Q(A) to Q(B), but slightly affected the donor side of PSII. Moreover, all of the three mutant cells were more sensitive to high light and became slower to recover from photoinhibition. In the isolated thylakoid membranes from the three mutants, the PsbU subunit was lost and the oxygen-evolving activity was reduced to a lower level compared to that in the respective cells. PSII complexes isolated from these mutants showed no oxygen-evolving activity, which was found to be due to large or complete loss of PsbO, PsbV and PsbU during the process of purification. Moreover, PSII cores purified from the three mutants contained Psb27, an assembly co-factor of PSII. These results suggest that PsbO-D158 is required for the proper binding of the three extrinsic proteins to PSII and plays an important role in maintaining the optimal oxygen-evolving activity, and its mutation caused incomplete assembly of the PSII complex. |
| Keyword | PsbO Site-directed mutagenesis Photosystem II Oxygen evolution Thermosynechococcus vulcanus |
| Subject Area | Plant Sciences |
| DOI | 10.1007/s11120-020-00715-0 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | MANGANESE-STABILIZING PROTEIN ; SITE-DIRECTED MUTANTS ; EXTRINSIC PROTEINS ; OXYGEN-EVOLUTION ; DELETION MUTAGENESIS ; WATER OXIDATION ; CLUSTER ; SUBUNIT ; POLYPEPTIDE ; ELONGATUS |
| WOS Research Area | Plant Sciences |
| WOS ID | WOS:000518147700001 |
| Publisher | SPRINGER |
| Special Issue | SI |
| Subtype | Article |
| Publication Place | DORDRECHT |
| EISSN | 1573-5079 |
| Funding Organization | National Key R&D Program of China [2017YFA0503700] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31470339] ; Strategic Priority Research Program of CAS [XDB17000000] ; Key Research Program of Frontier Sciences, CAS [QYZDY-SSW-SMC003] |
| Corresponding Author Email | shen@cc.okayama-u.ac.jp ; hanguangye@ibcas.ac.cn |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/21882 |
| Collection | 中科院光生物学重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Photosynth Res Ctr, Key Lab Photobiol, Inst Bot, 20 Nanxincun, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Yuquan Rd, Beijing 100049, Peoples R China 3.Okayama Univ, Res Inst Interdisciplinary Sci, Grad Sch Nat Sci & Technol, Tsushima Naka 3-1-1, Okayama 7008530, Japan |
| Recommended Citation GB/T 7714 | Zhu, Qingjun,Yang, Yanyan,Xiao, Yanan,et al. Function of PsbO-Asp158 in photosystem II: effects of mutation of this residue on the binding of PsbO and function of PSII in Thermosynechococcus vulcanus[J]. PHOTOSYNTHESIS RESEARCH,2020,146(1-3):29-40. |
| APA | Zhu, Qingjun.,Yang, Yanyan.,Xiao, Yanan.,Wang, Wenda.,Kuang, Tingyun.,...&Han, Guangye.(2020).Function of PsbO-Asp158 in photosystem II: effects of mutation of this residue on the binding of PsbO and function of PSII in Thermosynechococcus vulcanus.PHOTOSYNTHESIS RESEARCH,146(1-3),29-40. |
| MLA | Zhu, Qingjun,et al."Function of PsbO-Asp158 in photosystem II: effects of mutation of this residue on the binding of PsbO and function of PSII in Thermosynechococcus vulcanus".PHOTOSYNTHESIS RESEARCH 146.1-3(2020):29-40. |
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| Zhu-2020-Function of（2226KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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