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Function of PsbO-Asp158 in photosystem II: effects of mutation of this residue on the binding of PsbO and function of PSII in Thermosynechococcus vulcanus
Zhu, Qingjun1; Yang, Yanyan; Xiao, Yanan1; Wang, Wenda1; Kuang, Tingyun; Shen, Jian-Ren; Han, Guangye
2020
Source PublicationPHOTOSYNTHESIS RESEARCH
ISSN0166-8595
Volume146Issue:1-3Pages:29-40
AbstractPsbO-D158 is a highly conserved residue of the PsbO protein in photosystem II (PSII), and participates in one of the hydrogen-bonding networks connecting the manganese cluster with the lumenal surface. In order to examine the role of PsbO-D158, we mutated it to E, N or K in Thermosynechococcus vulcanus and characterized photosynthetic properties of the mutants obtained. The growth rates of these three mutants were similar to that of the wild type, whereas the oxygen-evolving activity of the three mutant cells decreased to 60-64% of the wild type. Fluorescence kinetics showed that the mutations did not affect the electron transfer from Q(A) to Q(B), but slightly affected the donor side of PSII. Moreover, all of the three mutant cells were more sensitive to high light and became slower to recover from photoinhibition. In the isolated thylakoid membranes from the three mutants, the PsbU subunit was lost and the oxygen-evolving activity was reduced to a lower level compared to that in the respective cells. PSII complexes isolated from these mutants showed no oxygen-evolving activity, which was found to be due to large or complete loss of PsbO, PsbV and PsbU during the process of purification. Moreover, PSII cores purified from the three mutants contained Psb27, an assembly co-factor of PSII. These results suggest that PsbO-D158 is required for the proper binding of the three extrinsic proteins to PSII and plays an important role in maintaining the optimal oxygen-evolving activity, and its mutation caused incomplete assembly of the PSII complex.
KeywordPsbO Site-directed mutagenesis Photosystem II Oxygen evolution Thermosynechococcus vulcanus
Subject AreaPlant Sciences
DOI10.1007/s11120-020-00715-0
Indexed BySCI
Language英语
WOS KeywordMANGANESE-STABILIZING PROTEIN ; SITE-DIRECTED MUTANTS ; EXTRINSIC PROTEINS ; OXYGEN-EVOLUTION ; DELETION MUTAGENESIS ; WATER OXIDATION ; CLUSTER ; SUBUNIT ; POLYPEPTIDE ; ELONGATUS
WOS Research AreaPlant Sciences
WOS IDWOS:000518147700001
PublisherSPRINGER
Special IssueSI
SubtypeArticle
Publication PlaceDORDRECHT
EISSN1573-5079
Funding OrganizationNational Key R&D Program of China [2017YFA0503700] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31470339] ; Strategic Priority Research Program of CAS [XDB17000000] ; Key Research Program of Frontier Sciences, CAS [QYZDY-SSW-SMC003]
Corresponding Author Emailshen@cc.okayama-u.ac.jp ; hanguangye@ibcas.ac.cn
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ibcas.ac.cn/handle/2S10CLM1/21882
Collection中科院光生物学重点实验室
Affiliation1.Chinese Acad Sci, Photosynth Res Ctr, Key Lab Photobiol, Inst Bot, 20 Nanxincun, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Yuquan Rd, Beijing 100049, Peoples R China
3.Okayama Univ, Res Inst Interdisciplinary Sci, Grad Sch Nat Sci & Technol, Tsushima Naka 3-1-1, Okayama 7008530, Japan
Recommended Citation
GB/T 7714
Zhu, Qingjun,Yang, Yanyan,Xiao, Yanan,et al. Function of PsbO-Asp158 in photosystem II: effects of mutation of this residue on the binding of PsbO and function of PSII in Thermosynechococcus vulcanus[J]. PHOTOSYNTHESIS RESEARCH,2020,146(1-3):29-40.
APA Zhu, Qingjun.,Yang, Yanyan.,Xiao, Yanan.,Wang, Wenda.,Kuang, Tingyun.,...&Han, Guangye.(2020).Function of PsbO-Asp158 in photosystem II: effects of mutation of this residue on the binding of PsbO and function of PSII in Thermosynechococcus vulcanus.PHOTOSYNTHESIS RESEARCH,146(1-3),29-40.
MLA Zhu, Qingjun,et al."Function of PsbO-Asp158 in photosystem II: effects of mutation of this residue on the binding of PsbO and function of PSII in Thermosynechococcus vulcanus".PHOTOSYNTHESIS RESEARCH 146.1-3(2020):29-40.
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