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| The transcription factor VaNAC17 from grapevine (Vitis amurensis) enhances drought tolerance by modulating jasmonic acid biosynthesis in transgenic Arabidopsis | |
Su, Lingye1,2,3; Fang, Linchuan1; Zhu, Zhenfei4; Zhang, Langlang1; Sun, Xiaoming1; Wang, Yi2,4; Wang, Qingfeng1; Li, Shaohua2 ; Xin, Haiping1
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| 2020 | |
| 发表期刊 | PLANT CELL REPORTS
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| ISSN | 0721-7714 |
| 卷号 | 39期号:5页码:621-634 |
| 摘要 | Key message Expression of VaNAC17 improved drought tolerance in transgenic Arabidopsis by upregulating stress-responsive genes, modulating JA biosynthesis, and enhancing ROS scavenging. Water deficit severely affects the growth and development of plants such as grapevine (Vitis spp.). Members of the NAC (NAM, ATAF1/2, and CUC2) transcription factor (TF) family participate in drought-stress-induced signal transduction in plants, but little is known about the roles of NAC genes in drought tolerance in grapevine. Here, we explored the role of VaNAC17 in Vitis amurensis, a cold-hardy, drought-tolerant species of grapevine. VaNAC17 was strongly induced in grapevine by drought, exogenous abscisic acid (ABA), and methyl jasmonate (MeJA). A transient expression assay in yeast indicated that VaNAC17 functions as a transcriptional activator. Notably, heterologous expression of VaNAC17 in Arabidopsis thaliana enhanced drought tolerance. VaNAC17-expressing Arabidopsis plants showed decreased reactive oxygen species (ROS) accumulation compared to wild-type plants under drought conditions. RNA-seq analysis indicated that VaNAC17 expression increased the transcription of downstream stress-responsive genes after 5 days of drought treatment, especially genes involved in jasmonic acid (JA) biosynthesis (such as LOX3, AOC1 and OPR3) and signaling (such as MYC2, JAZ1, VSP1 and CORI3) pathways. Endogenous JA levels increased in VaNAC17-OE plants under drought stress. Taken together, these results indicate that VaNAC17 plays a positive role in drought tolerance by modulating endogenous JA biosynthesis and ROS scavenging. |
| 关键词 | Drought stress Jasmonic acid VaNAC17 Vitis amurensis |
| 学科领域 | Plant Sciences |
| DOI | 10.1007/s00299-020-02519-x |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | STRESS-RESPONSE ; ABSCISIC-ACID ; GENE FAMILY ; OSMOTIC-STRESS ; PLANT STRESS ; NAC ; EXPRESSION ; GROWTH ; DEFENSE ; REGULATOR |
| WOS研究方向 | Plant Sciences |
| WOS记录号 | WOS:000516940700001 |
| 出版者 | SPRINGER |
| 文献子类 | Article |
| 出版地 | NEW YORK |
| EISSN | 1432-203X |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31672132] ; Youth Innovation Promotion Association of Chinese Academy of Sciences [2015281] ; Grape Breeding Project of Ningxia [NXNYYZ201502] |
| 作者邮箱 | sulingye@163.com ; fanglincuan@126.com ; zhuzhenfei16@mails.ucas.ac.cn ; zll88927@126.com ; aspire2018@163.com ; wangyi19881107@163.com ; qfwang@wbgcas.cn ; shhli@ibcas.ac.cn ; xinhaiping215@hotmail.com |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/21890 |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Sino Africa Joint Res Ctr, Wuhan Bot Garden, Wuhan, Peoples R China 2.Chinese Acad Sci, Ctr Econ Bot, Core Bot Gardens, Wuhan, Peoples R China 3.Chinese Acad Sci, Beijing Key Lab Grape Sci & Enol, Lab Plant Resources, Inst Bot, Beijing, Peoples R China 4.Guangdong Acad Forestry, Guangdong Prov Key Lab Silviculture Protect & Uti, Guangzhou, Peoples R China 5.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Su, Lingye,Fang, Linchuan,Zhu, Zhenfei,et al. The transcription factor VaNAC17 from grapevine (Vitis amurensis) enhances drought tolerance by modulating jasmonic acid biosynthesis in transgenic Arabidopsis[J]. PLANT CELL REPORTS,2020,39(5):621-634. |
| APA | Su, Lingye.,Fang, Linchuan.,Zhu, Zhenfei.,Zhang, Langlang.,Sun, Xiaoming.,...&Xin, Haiping.(2020).The transcription factor VaNAC17 from grapevine (Vitis amurensis) enhances drought tolerance by modulating jasmonic acid biosynthesis in transgenic Arabidopsis.PLANT CELL REPORTS,39(5),621-634. |
| MLA | Su, Lingye,et al."The transcription factor VaNAC17 from grapevine (Vitis amurensis) enhances drought tolerance by modulating jasmonic acid biosynthesis in transgenic Arabidopsis".PLANT CELL REPORTS 39.5(2020):621-634. |
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