Knowledge Management System Of Institute Of Botany,CAS
Nonlinear responses of the V-max and K-m of hydrolytic and polyphenol oxidative enzymes to nitrogen enrichment | |
Liu, Weixing; Tian, Rui1; Peng, Ziyang; Yang, Sen2; Liu, Xiao Xiao1; Yang, Yashu3; Zhang, Wenhao2; Liu, Lingli2 | |
2020 | |
发表期刊 | SOIL BIOLOGY & BIOCHEMISTRY |
ISSN | 0038-0717 |
卷号 | 141 |
摘要 | The kinetics of extracellular enzymes produced by soil microorganisms regulate soil organic matter decomposition and other ecosystem functions. Atmospheric nitrogen (N) deposition creates a soil environment with elevated reactive N, which is expected to affect the kinetic parameters (V-max and K-m) of hydrolytic and poly-phenol oxidative enzymes, as well as soil microbial respiration. We measured the V-max and K-m of seven soil hydrolytic enzymes and polyphenol oxidase (PPO), as well as soil microbial respiration and physiochemical properties, in a temperate steppe after 15 years of multi-level N addition treatments. As the N addition increased, the V-max of most carbon (C) -degrading and N-degrading hydrolytic enzymes decreased, and this was associated with a decline in soil pH. In contrast, the V-max of acid phosphatase (AP) increased with increasing N addition. Higher N addition was associated with lower K-m of most hydrolytic enzymes, expect for AP and beta-xylosidase (BX). There was a quadratic relationship between N addition and the V-max and K-m of PPO, which reached a maximum with the addition of 8 g ;N m(-2) y(-1), and decreased at higher N addition levels. Structural equation modeling indicated that the decline in soil microbial respiration with increasing N deposition was directly mediated by the BG kinetics, due to the fact N-induced acidification negatively impacted the V-max and K-m of BG. Our empirical data on soil enzyme (i.e., V-max, K-m) and its relationship with microbial respiration should be useful for modelling how microbes and substrates interact to regulate soil carbon cycling under N enrichment. |
关键词 | Acidification Extracellular enzyme kinetics Microbial biomass Microbial decomposition Nitrogen fertilization |
学科领域 | Soil Science |
DOI | 10.1016/j.soilbio.2019.107656 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | SOIL CARBON STORAGE ; PHOSPHORUS LIMITATION ; MICROBIAL BIOMASS ; LITTER DECAY ; DEPOSITION ; GRASSLAND ; DECOMPOSITION ; MECHANISMS ; KINETICS ; FERTILIZATION |
WOS研究方向 | Agriculture |
WOS记录号 | WOS:000513986700002 |
出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
文献子类 | Article |
出版地 | OXFORD |
资助机构 | National Key Research and Development Program of China [2016YFC0500701] ; Strategic Priority Research, Chinese Academy of Sciences [XDA23080301] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31770530, 31370488] |
作者邮箱 | Lingli.liu@ibcas.ac.cn |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/21902 |
专题 | 植被与环境变化国家重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China 2.Henan Univ, Int Joint Res Lab Global Change Ecol, Coll Life Sci, Kaifeng 475004, Henan, Peoples R China 3.Univ Chinese Acad Sci, Yuquan Rd, Beijing 100049, Peoples R China 4.Shandong Agr Univ, Coll Plant Protect, Tai An 271018, Shandong, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, Weixing,Tian, Rui,Peng, Ziyang,et al. Nonlinear responses of the V-max and K-m of hydrolytic and polyphenol oxidative enzymes to nitrogen enrichment[J]. SOIL BIOLOGY & BIOCHEMISTRY,2020,141. |
APA | Liu, Weixing.,Tian, Rui.,Peng, Ziyang.,Yang, Sen.,Liu, Xiao Xiao.,...&Liu, Lingli.(2020).Nonlinear responses of the V-max and K-m of hydrolytic and polyphenol oxidative enzymes to nitrogen enrichment.SOIL BIOLOGY & BIOCHEMISTRY,141. |
MLA | Liu, Weixing,et al."Nonlinear responses of the V-max and K-m of hydrolytic and polyphenol oxidative enzymes to nitrogen enrichment".SOIL BIOLOGY & BIOCHEMISTRY 141(2020). |
条目包含的文件 | ||||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
1-s2.0-S003807171930(1152KB) | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论