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| Knockout of VvCCD8 gene in grapevine affects shoot branching | |
Ren, Chong1; Guo, Yuchen1,2; Kong, Junhua1; Lecourieux, Fatma3; Dai, Zhanwu1 ; Li, Shaohua1; Liang, Zhenchang4
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| 2020 | |
| Source Publication | BMC PLANT BIOLOGY
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| ISSN | 1471-2229 |
| Volume | 20Issue:1 |
| Abstract | Background Shoot branching is an important trait of plants that allows them to adapt to environment changes. Strigolactones (SLs) are newly identified plant hormones that inhibit shoot branching in plants. The SL biosynthesis genes CCD7 (carotenoid cleavage dioxygenase 7) and CCD8 have been found to regulate branching in several herbaceous plants by taking advantage of their loss-of-function mutants. However, the role for CCD7 and CCD8 in shoot branching control in grapevine is still unknown due to the lack of corresponding mutants. Results Here we employed the CRISPR/Cas9 system to edit the VvCCD7 and VvCCD8 genes in the grape hybrid 41B. The 41B embryogenic cells can easily be transformed and used for regeneration of the corresponding transformed plants. Sequencing analysis revealed that gene editing has been used successfully to target both VvCCD genes in 41B embryogenic cells. After regeneration, six 41B plantlets were identified as transgenic plants carrying the CCD8-sgRNA expression cassette. Among these, four plants showed mutation in the target region and were selected as ccd8 mutants. These ccd8 mutants showed increased shoot branching compared to the corresponding wild-type plants. In addition, no off-target mutation was detected in the tested mutants at predicted off-target sites. Conclusions Our results underline the key role of VvCCD8 in the control of grapevine shoot branching. |
| Keyword | CRISPR Cas9 CCD8 Mutant Shoot branching Strigolactone |
| Subject Area | Plant Sciences |
| DOI | 10.1186/s12870-020-2263-3 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | BUD OUTGROWTH ; STRIGOLACTONE BIOSYNTHESIS ; ARABIDOPSIS ; INHIBITION ; CYTOKININ ; EXPRESSION ; RELEASE ; SIGNALS ; ACTS ; PCR |
| WOS Research Area | Plant Sciences |
| WOS ID | WOS:000513889400003 |
| Publisher | BMC |
| Subtype | Article |
| Publication Place | LONDON |
| Funding Organization | major science and technology program of NingxiaHui Autonomous region [2016BZ06] ; National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31772266] ; STS project of Chinese Academy of Sciences [KFJ-STS-ZDTP-025] ; Agricultural Breeding Project of Ningxia Hui Autonomous Region [NXNYYZ20150203] |
| Corresponding Author Email | zl249@ibcas.ac.cn |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/21907 |
| Collection | 中科院北方资源植物重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Beijing Key Lab Grape Sci & Enol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, CAS Key Lab Plant Resources, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Univ Bordeaux, EGFV, Bordeaux Sci Agro, INRA,ISVV, F-33140 Bordeaux, France 5.Chinese Acad Sci, Inst Bot, Nanxin Village 20, Beijing 100093, Peoples R China |
| Recommended Citation GB/T 7714 | Ren, Chong,Guo, Yuchen,Kong, Junhua,et al. Knockout of VvCCD8 gene in grapevine affects shoot branching[J]. BMC PLANT BIOLOGY,2020,20(1). |
| APA | Ren, Chong.,Guo, Yuchen.,Kong, Junhua.,Lecourieux, Fatma.,Dai, Zhanwu.,...&Liang, Zhenchang.(2020).Knockout of VvCCD8 gene in grapevine affects shoot branching.BMC PLANT BIOLOGY,20(1). |
| MLA | Ren, Chong,et al."Knockout of VvCCD8 gene in grapevine affects shoot branching".BMC PLANT BIOLOGY 20.1(2020). |
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| File Name/Size | DocType | Version | Access | License | ||
| Ren-2020-Knockout of（1253KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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