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Knockout of VvCCD8 gene in grapevine affects shoot branching
Ren, Chong1; Guo, Yuchen1,2; Kong, Junhua1; Lecourieux, Fatma3; Dai, Zhanwu1; Li, Shaohua1; Liang, Zhenchang4
Source PublicationBMC PLANT BIOLOGY
AbstractBackground Shoot branching is an important trait of plants that allows them to adapt to environment changes. Strigolactones (SLs) are newly identified plant hormones that inhibit shoot branching in plants. The SL biosynthesis genes CCD7 (carotenoid cleavage dioxygenase 7) and CCD8 have been found to regulate branching in several herbaceous plants by taking advantage of their loss-of-function mutants. However, the role for CCD7 and CCD8 in shoot branching control in grapevine is still unknown due to the lack of corresponding mutants. Results Here we employed the CRISPR/Cas9 system to edit the VvCCD7 and VvCCD8 genes in the grape hybrid 41B. The 41B embryogenic cells can easily be transformed and used for regeneration of the corresponding transformed plants. Sequencing analysis revealed that gene editing has been used successfully to target both VvCCD genes in 41B embryogenic cells. After regeneration, six 41B plantlets were identified as transgenic plants carrying the CCD8-sgRNA expression cassette. Among these, four plants showed mutation in the target region and were selected as ccd8 mutants. These ccd8 mutants showed increased shoot branching compared to the corresponding wild-type plants. In addition, no off-target mutation was detected in the tested mutants at predicted off-target sites. Conclusions Our results underline the key role of VvCCD8 in the control of grapevine shoot branching.
KeywordCRISPR Cas9 CCD8 Mutant Shoot branching Strigolactone
Subject AreaPlant Sciences
Indexed BySCI
WOS Research AreaPlant Sciences
WOS IDWOS:000513889400003
Publication PlaceLONDON
Funding Organizationmajor science and technology program of NingxiaHui Autonomous region [2016BZ06] ; National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31772266] ; STS project of Chinese Academy of Sciences [KFJ-STS-ZDTP-025] ; Agricultural Breeding Project of Ningxia Hui Autonomous Region [NXNYYZ20150203]
Corresponding Author Emailzl249@ibcas.ac.cn
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Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Affiliation1.Chinese Acad Sci, Beijing Key Lab Grape Sci & Enol, Beijing 100093, Peoples R China
2.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, CAS Key Lab Plant Resources, Beijing 100093, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Univ Bordeaux, EGFV, Bordeaux Sci Agro, INRA,ISVV, F-33140 Bordeaux, France
5.Chinese Acad Sci, Inst Bot, Nanxin Village 20, Beijing 100093, Peoples R China
Recommended Citation
GB/T 7714
Ren, Chong,Guo, Yuchen,Kong, Junhua,et al. Knockout of VvCCD8 gene in grapevine affects shoot branching[J]. BMC PLANT BIOLOGY,2020,20(1).
APA Ren, Chong.,Guo, Yuchen.,Kong, Junhua.,Lecourieux, Fatma.,Dai, Zhanwu.,...&Liang, Zhenchang.(2020).Knockout of VvCCD8 gene in grapevine affects shoot branching.BMC PLANT BIOLOGY,20(1).
MLA Ren, Chong,et al."Knockout of VvCCD8 gene in grapevine affects shoot branching".BMC PLANT BIOLOGY 20.1(2020).
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