Development of a PVY Resistant Flue-Cured Tobacco Line via EMS Mutagenesis of eIF4E

doi:10.3390/agronomy10010036

	Development of a PVY Resistant Flue-Cured Tobacco Line via EMS Mutagenesis of eIF4E
	Zhao, Lu 1,2; Li, Wenzheng 1,2; Wang, Bingwu 1,2; Gao, Yulong 1,2; Sui, Xueyi 1,2; Liu, Yong 1,2; Chen, Xuejun 1,2; Yao, Xuefeng 3,4; Jiao, Fangchan 1,2; Song, Zhongbang 1,2
	2020
发表期刊	AGRONOMY-BASEL
卷号	10 期号:1
摘要	Recessive resistance against potyviruses, such as Potato virus Y (PVY), relies on mutations in the eukaryotic translation initiation factor 4E (eIF4E) or one of its isoforms. The eIF4E1-S mutants of burley tobacco (Nicotiana tabacum L.) exhibit recessive resistance against PVY strains. Here, we developed a TILLING population of flue-cured tobacco (N. tabacum cv. Yunyan87) using ethyl methanesulfonate (EMS) to identify eIF4E1-S mutants. M3 plants homozygous for a nonsense mutation in exon 1 of the eIF4E1-S gene demonstrated resistance against PVYMN. These M3 plants were backcrossed to 'Yunyan87', and BC4F3 plants were screened using derived cleaved amplified polymorphic sequence (dCAPS) markers. BC(4)F(3)( )plants showing agronomic traits comparable to the recurrent parent 'Yunyan87' and resistance against PVYO, PVYN, and PVYNTN strains were identified. These genotypes would provide useful germplasm for future tobacco improvement and would aid in basic research on PVY resistance in flue-cured tobacco.
关键词	eIF4E1-S potyvirus strains Nicotiana tabacum cv. Yunyan87 EMS-induced nonsense mutation TILLING dCAPS markers
学科领域	Agronomy ; Plant Sciences
DOI	10.3390/agronomy10010036
收录类别	SCI
语种	英语
WOS关键词	POTATO-VIRUS-Y ; RECESSIVE RESISTANCE ; ARABIDOPSIS-THALIANA ; POTYVIRUS RESISTANCE ; GLANDULAR TRICHOMES ; INITIATION-FACTORS ; NATURAL VARIATION ; GENE ; SUSCEPTIBILITY ; EIF(ISO)4E
WOS研究方向	Agriculture ; Plant Sciences
WOS记录号	WOS:000513232600036
出版者	MDPI
文献子类	Article
出版地	BASEL
EISSN	2073-4395
资助机构	Yunnan Academy of Tobacco Agricultural Sciences [2012YN02, 2017YN02, 2017YN03]
作者邮箱	zhaolu66@outlook.com ; lwz67@163.com ; bwwang76@hotmail.com ; gyl3000@163.com ; xueyisui2017@hotmail.com ; yong2liu@sina.com ; cxjkm@163.com ; yxfun@163.com ; jfc99002@163.com ; zbsoon@vip.163.com
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/21914
专题	中科院植物分子生理学重点实验室
作者单位	1.Yunnan Acad Tobacco Agr Sci, Tobacco Breeding & Biotechnol Res Ctr, Kunming 650021, Yunnan, Peoples R China 2.Key Lab Tobacco Biotechnol Breeding, Kunming 650021, Yunnan, Peoples R China 3.Natl Tobacco Genet Engn Res Ctr, Kunming 650021, Yunnan, Peoples R China 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Lu,Li, Wenzheng,Wang, Bingwu,et al. Development of a PVY Resistant Flue-Cured Tobacco Line via EMS Mutagenesis of eIF4E[J]. AGRONOMY-BASEL,2020,10(1).
APA	Zhao, Lu.,Li, Wenzheng.,Wang, Bingwu.,Gao, Yulong.,Sui, Xueyi.,...&Song, Zhongbang.(2020).Development of a PVY Resistant Flue-Cured Tobacco Line via EMS Mutagenesis of eIF4E.AGRONOMY-BASEL,10(1).
MLA	Zhao, Lu,et al."Development of a PVY Resistant Flue-Cured Tobacco Line via EMS Mutagenesis of eIF4E".AGRONOMY-BASEL 10.1(2020).

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